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. 1984 Mar 30;223(4643):1412-4.
doi: 10.1126/science.6367046.

Biological activity of recombinant human interleukin-2 produced in Escherichia coli

S A Rosenberg, E A Grimm, M McGrogan, M Doyle, E Kawasaki, K Koths, D F Mark

Biological activity of recombinant human interleukin-2 produced in Escherichia coli

S A Rosenberg et al. Science. .

Abstract

The gene for interleukin-2 was isolated from the Jurkat cell line and from normal peripheral blood lymphocytes and, when inserted in Escherichia coli, was expressed at high concentrations. This interleukin-2 was purified to apparent homogeneity and tested for biological activity in a variety of assays in vitro and in vivo. The recombinant lymphokine supports the growth of murine and human interleukin-2 dependent cell lines, enhances the generation of murine and human cytolytic cells in vitro, and generates lymphokine activated killer cells from murine and human lymphocytes. It has a serum half-life of 2 to 3 minutes in the mouse and significantly enhances the generation of cytolytic cells in vivo after alloimmunization. No functional differences between native and the recombinant interleukin-2 molecules have been detected.

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