Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Jun 1;181(6):2141-51.
doi: 10.1084/jem.181.6.2141.

Suppression of erythro-megakaryocytopoiesis and the induction of reversible thrombocytopenia in mice transgenic for the thymidine kinase gene _targeted by the platelet glycoprotein alpha IIb promoter

D Tronik-Le Roux  1 , V Roullot, A Schweitzer, R Berthier, G Marguerie
Affiliations

Suppression of erythro-megakaryocytopoiesis and the induction of reversible thrombocytopenia in mice transgenic for the thymidine kinase gene _targeted by the platelet glycoprotein alpha IIb promoter

D Tronik-Le Roux et al. J Exp Med. .

Erratum in

  • J Exp Med 1995 Oct 1;182(4):1177

Abstract

The mechanisms that regulate the commitment of a totipotent stem cell to the megakaryocytic lineage are largely unknown. Using a molecular approach to the study of megakaryocytopoiesis and platelet production, mice in which thrombocytopoiesis could be controlled were produced by _targeting the expression of the herpes simplex virus thymidine kinase toxigene to megakaryocytes using the regulatory region of the gene encoding the alpha subunit of the platelet integrin alpha IIb beta 3. The programmed eradication of the megakaryocytic lineage was induced by treating transgenic mice bearing the hybrid construct (alpha IIbtk) with the antiherpetic drug ganciclovir (GCV). After 10 d of treatment, the platelet number was reduced by > 94.6%. After discontinuing GCV, the bone marrow was repopulated with megakaryocytes and the platelet count was restored within 7 d. Prolonged GCV treatment induced erythropenia in the transgenic mice. Assays of myeloid progenitor cells in vitro demonstrated that the transgene was expressed in early erythro-megakaryocytic progenitor cells. The reversibility and facility of this system provides a powerful model to determine both the critical events in megakaryocytic and erythroid lineage development and for evaluating the precise role that platelets play in the pathogenesis of a number of vascular occlusive disorders.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nature. 1990 Mar 29;344(6265):444-7 - PubMed
    1. Nature. 1990 Mar 29;344(6265):447-9 - PubMed
    1. Biochemistry. 1990 Feb 6;29(5):1232-44 - PubMed
    1. Leuk Res. 1990;14(6):495-9 - PubMed
    1. Blood. 1990 Oct 1;76(7):1330-5 - PubMed

Publication types

MeSH terms

  NODES
twitter 2