doi: 10.1099/00222615-41-4-231.
Detection of Streptococcus mutans by PCR amplification of spaP gene
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- PMID: 7932616
- DOI: 10.1099/00222615-41-4-231
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Detection of Streptococcus mutans by PCR amplification of spaP gene
J Med Microbiol.
1994 Oct.
Abstract
Synthetic oligonucleotide primers were used in the polymerase chain reaction (PCR) to amplify a sequence of the spaP gene, which encodes the surface protein antigen I/II of Streptococcus mutans. A DNA fragment of c. 192 bp was amplified from lysed S. mutans cells or isolated DNA. With S. mutans cells, the lower limit of detection was 4-40 cfu. With these primers, 13 reference and 50 clinical strains of S. mutans were identified. Amplification of the 192-bp product was not demonstrated when 41 strains of other streptococcal and non-streptococcal species were tested. The spaP gene PCR has potential for the rapid diagnosis of S. mutans infections.
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