Mutations in the nuclear export signal of human ran-binding protein RanBP1 block the Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1
- PMID: 9111043
- DOI: 10.1074/jbc.272.17.11356
Mutations in the nuclear export signal of human ran-binding protein RanBP1 block the Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1
Abstract
We identified a region in the human Ran GTPase-binding protein RanBP1 that shares similarities to the nuclear export signal of the inhibitor of the cAMP-dependent protein kinase. Mutational analysis confirmed that this region is responsible for the cytoplasmic accumulation of RanBP1 and can functionally replace the nuclear export signal of Rev of human immunodeficiency virus type 1. We showed that RanBP1 interferes with Rev-mediated expression of human immunodeficiency virus type 1, whereas the RanBP1 with inactivated nuclear export signal abrogates Rev function. Expression of a Rev-independent molecular clone, which is regulated via the constitutive transport element (CTE) of the simian retrovirus type 1, is not affected. These findings indicate that Rev and RanBP1 compete for the same nuclear export pathway, whereas Rev- and the CTE-mediated pathways are distinct. The inhibition of Rev function is independent of the ability of RanBP1 to associate with Ran and therefore, it is not likely a result of interference with Ran function. These data suggest that RanBP1 interacts with Rev at the putative nuclear receptor and, hence, shares a step in posttranscriptional pathway with Rev.
Similar articles
-
The simian retrovirus-1 constitutive transport element, unlike the HIV-1 RRE, uses factors required for cellular mRNA export.Curr Biol. 1997 Sep 1;7(9):619-28. doi: 10.1016/s0960-9822(06)00288-0. Curr Biol. 1997. PMID: 9285715
-
Interactions between HIV Rev and nuclear import and export factors: the Rev nuclear localisation signal mediates specific binding to human importin-beta.J Mol Biol. 1997 Dec 19;274(5):693-707. doi: 10.1006/jmbi.1997.1420. J Mol Biol. 1997. PMID: 9405152
-
Inhibition of human immunodeficiency virus Rev and human T-cell leukemia virus Rex function, but not Mason-Pfizer monkey virus constitutive transport element activity, by a mutant human nucleoporin _targeted to Crm1.J Virol. 1998 Nov;72(11):8627-35. doi: 10.1128/JVI.72.11.8627-8635.1998. J Virol. 1998. PMID: 9765402 Free PMC article.
-
Nucleocytoplasmic RNA transport in retroviral replication.Results Probl Cell Differ. 2001;34:197-217. doi: 10.1007/978-3-540-40025-7_12. Results Probl Cell Differ. 2001. PMID: 11288676 Review.
-
The HIV-1 Rev protein.Annu Rev Microbiol. 1998;52:491-532. doi: 10.1146/annurev.micro.52.1.491. Annu Rev Microbiol. 1998. PMID: 9891806 Review.
Cited by
-
Persistent infection of rhesus macaques by the rev-independent Nef(-) simian immunodeficiency virus SIVmac239: replication kinetics and genomic stability.J Virol. 1999 Jul;73(7):6159-65. doi: 10.1128/JVI.73.7.6159-6165.1999. J Virol. 1999. PMID: 10364376 Free PMC article.
-
Inhibition of hepatitis B virus replication by MyD88 involves accelerated degradation of pregenomic RNA and nuclear retention of pre-S/S RNAs.J Virol. 2010 Jul;84(13):6387-99. doi: 10.1128/JVI.00236-10. Epub 2010 Apr 21. J Virol. 2010. PMID: 20410269 Free PMC article.
-
Facilitated nucleocytoplasmic shuttling of the Ran binding protein RanBP1.Mol Cell Biol. 2000 May;20(10):3510-21. doi: 10.1128/MCB.20.10.3510-3521.2000. Mol Cell Biol. 2000. PMID: 10779340 Free PMC article.
-
Yeast Ran-binding protein 1 (Yrb1) shuttles between the nucleus and cytoplasm and is exported from the nucleus via a CRM1 (XPO1)-dependent pathway.Mol Cell Biol. 2000 Jun;20(12):4295-308. doi: 10.1128/MCB.20.12.4295-4308.2000. Mol Cell Biol. 2000. PMID: 10825193 Free PMC article.
-
TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus.EMBO J. 1999 Apr 1;18(7):1953-65. doi: 10.1093/emboj/18.7.1953. EMBO J. 1999. PMID: 10202158 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous
