Net K + secretion in the thick ascending limb of mice on a low-Na, high-K diet

Wang, Bangchen; Wen, Duo; Li, Huaqing; Wang-France, Jun; Sansom, Steven C.

doi:10.1016/j.kint.2017.04.009

Cited by 18 publications

(28 citation statements)

References 56 publications

(75 reference statements)

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“…Furosemide was given in vivo to determine whether the TAL was inhibited in mice on a LNaHK diet. Furosemide was found to inhibit the TAL in mice on a LNaHK diet, with the osmolality decreasing from 2,555 Ϯ 522 to 717 Ϯ 15 mosmol/L and the Na ϩ output increasing from 9.0 Ϯ 1.4 to 98.8 Ϯ 20.4 (160). This magnitude of change of Na ϩ and osmolality was the same as found for furosemide-treated mice on a regular diet (160).…”

Section: Nkcc2 and Ncc Pathwayssupporting

confidence: 63%

Maintaining K⁺balance on the low-Na⁺, high-K⁺diet

Cornelius

Wang

Wang-France

et al. 2016

American Journal of Physiology-Renal Physiology

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A low-Na, high-K diet (LNaHK) is considered a healthier alternative to the "Western" high-Na diet. Because the mechanism for K secretion involves Na reabsorptive exchange for secreted K in the distal nephron, it is not understood how K is eliminated with such low Na intake. Animals on a LNaHK diet produce an alkaline load, high urinary flows, and markedly elevated plasma ANG II and aldosterone levels to maintain their K balance. Recent studies have revealed a potential mechanism involving the actions of alkalosis, urinary flow, elevated ANG II, and aldosterone on two types of K channels, renal outer medullary K and large-conductance K channels, located in principal and intercalated cells. Here, we review these recent advances.

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Section: Nkcc2 and Ncc Pathwayssupporting

confidence: 63%

Maintaining K⁺balance on the low-Na⁺, high-K⁺diet

Cornelius

Wang

Wang-France

et al. 2016

American Journal of Physiology-Renal Physiology

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“…This response was not present in mice maintained on high K + alone. The data suggest that a high K + diet induces a net K + movement in the secretory direction and that this kaliuresis induced by furosemide only occurs when NKCC2 activity is sufficiently stimulated . Importantly, the response to furosemide observed on low Na + /high K + diet was completely absent in ROMK‐deficient mice, clearly delineating that ROMK is critical for K + secretion in the segment.…”

Section: Axial and Cellular Heterogeneity Of K+ Transport In Talmentioning

confidence: 77%

“…In contrast, K + appeared to be secreted from the TAL in rats maintained on a high K + diet. Wang et al recently found that the kaliuretic response to furosemide differed markedly when examining animals kept on a control diet or a low Na + /high K + diet . When mice were maintained on a control diet, blockade of NKCC2 by furosemide caused K + wasting and an increased K + delivery to the distal puncture site, likely reflecting inhibition of K + reabsorption in the TAL.…”

Section: Axial and Cellular Heterogeneity Of K+ Transport In Talmentioning

confidence: 86%

“…The molecular identity of these channels remains to be fully resolved. Early studies by Lu et al have found both channels to be absent in ROMK ‐ deficient mice, while in a recent study only the 70 pS channel was absent . Again these analyses may be complicated by the heterogeneous cellular makeup of the TAL, and hence, the difficulty to determine the identity of the cells studied by patch‐clamp.…”

Section: Axial and Cellular Heterogeneity Of K+ Transport In Talmentioning

confidence: 89%

“…Compared to a normal K + diet, a high dietary K + intake is associated with an increased number of patches containing 70 pS channels and a decreased number of 30 pS channels . Functional analysis in TAL cells from animals kept on a low Na + /high K + diet found augmented open probability of 70 pS channel, while no change in open probability of the 30 pS channel was observed …”

Section: Axial and Cellular Heterogeneity Of K+ Transport In Talmentioning

confidence: 99%

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Axial and cellular heterogeneity in electrolyte transport pathways along the thick ascending limb

Dimke

Schnermann

2018

Acta Physiologica

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The thick ascending limb (TAL) extends from the border of the inner medulla to the renal cortex, thus ascending through regions with wide differences in tissue solute and electrolyte concentrations. Structural and functional differences between TAL cells in the medulla (mTAL) and the cortex (cTAL) would therefore be useful to adapt TAL transport function to a changing external fluid composition. While mechanisms common to all TAL cells play a central role in the reclamation of about 25% of the NaCl filtered by the kidney, morphological features, Na / K -ATPase activity, NKCC2 splicing and phosphorylation do vary between segments and cells. The TAL contributes to K homeostasis and TAL cells with high or low basolateral K conductances have been identified which may be involved in K reabsorption and secretion respectively. Although transport rates for HCO3- do not differ between mTAL and cTAL, divergent axial and cellular expression of H transport proteins in TAL have been documented. The reabsorption of the divalent cations Ca and Mg is highest in cTAL and paralleled by differences in divalent cation permeability and the expression of select claudins. Morphologically, two cell types with different cell surface phenotypes have been described that still need to be linked to specific functional characteristics. The unique external environment and its change along the longitudinal axis require an axial functional heterogeneity for the TAL to optimally participate in conserving electrolyte homeostasis. Despite substantial progress in understanding TAL function, there are still considerable knowledge gaps that are just beginning to become bridged.

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