A Microfluidic Platform for Detection and Quantification of Two Biomarkers for Rheumatoid Arthritis

Early detection of rheumatoid factors (RF) and cyclic citrullinated peptide antibodies (anti-CCP) has been proven to be effective in diagnosing, and even preventing progression to more advanced-stages, of rheumatoid arthritis (RA), thereby decreasing irreversible damage to joints. Herein a novel, dual-_target detection approach was integrated in a 45 mm x 62 mm microfluidic chip that featured a symmetrical design in which magnetic beads surface-coated with fragment-crystallizable regions of immunoglobulin G and biotinylated CCP enabled detection of these two RA biomarkers within 55 min with only 5 mL of serum. This device could therefore be useful for rapid clinical diagnosis of this all-too-common ailment.

Note:
Funding Declaration: The authors thank the Ministry of Science and Technology (MOST) of Taiwan for funding this work (MOST 109B0247J3 to GBL, MOST 109-2224-E-007-002 to GBL, MOST 109- 3114-Y-001-001 to GBL, 109Q22803E1 to GBL, & 110Q2804E1 to GBL). We are also grateful for the funding from the CGMH-NTHU Joint Research Program (CGMH-NTHU-111- CORPG3M0071 to CCH, CMRPG8K0501 to FCK, & CMRPG8K0502 to FCK).

Conflict of Interest: The authors declare that there are no known competitive financial, work-related, or personal conflicts of interest related to this article.

Ethics: Twenty serum (waste) samples from KCGMH and four clinical samples (diluted 100-fold in EliA sample diluent from TFS) from Taoyuan Linkou Chang Gung Memorial Hospital (Taiwan; IRB no. 202101254B0)

Keywords: anti-cyclic citrullinated peptide, enzyme-linked immunosorbent assay (ELISA), microfluidics, rheumatoid arthritis, rheumatoid factor

Suggested Citation: Suggested Citation

Lee, Gwo-Bin and Wu, Tsung-Hsien and Tsai, Yi-Cheng and Kuo, Feng-Chih and Lee, Mel S. and Hu, Chih-Chien, A Microfluidic Platform for Detection and Quantification of Two Biomarkers for Rheumatoid Arthritis. Available at SSRN: https://ssrn.com/abstract=4328231 or http://dx.doi.org/10.2139/ssrn.4328231