Veterinární medicína, 2017 (vol. 62), issue 4
Comparative use of dimethyl sulphoxide (DMSO) in different animal speciesReview
G.M. Madruga, L.Z. Crivellenti, S. Borin-Crivellenti, C.A. Cintra, L.G. Gomes, P.R. Spiller
Vet Med - Czech, 2017, 62(4):179-185 | DOI: 10.17221/176/2015-VETMED 
Dimethyl sulphoxide has a variety of biological effects that have made it the subject of numerous pharmacological studies. The first dimethyl sulphoxide therapeutic indication approved by the United States Food and Drug Administration (FDA) in 1978 was for intravesical instillation in cases of interstitial cystitis. Since then, due to its distinctive properties, it has been the subject of studies in several areas. This review describes indications, adverse effects and contraindications, as takes a critical approach to the main articles addressing the clinical use of dimethyl sulphoxide.
Factors influencing reproductive efficiency and pregnancy in Pura Raza Española maresOriginal Paper
A. Akourki, A. Echegaray, M.V. Falceto, O. Mitjana
Vet Med - Czech, 2017, 62(4):186-191 | DOI: 10.17221/205/2015-VETMED
The aim of this study was to analyse ovarian activity and pregnancy in 140 Pura Raza Espanola mares over a period of two consecutive years. The data were analysed using SPSS software to evaluate the effects of age, category of mares and season on pre-ovulatory follicle size, ovulation rate, interval from foaling to pregnancy, gestation length, incidence of twin pregnancies and pregnancy losses. The results showed that follicular diameter was significantly (P < 0.05) smaller in fillies (3-year-old) and maiden mares than in older and multiparous mares. Within the group of animals treated with gonadotropin hormone hCG, 87.23% ovulated 48 h...
Fitness improvement of show jumping horses with deep water treadmill trainingOriginal Paper
A. Vincze, C. Szabo, S. Veres, D. Uto, A.T. Hevesi
Vet Med - Czech, 2017, 62(4):192-199 | DOI: 10.17221/135/2016-VETMED
Athletes, including equine athletes need high intensity training in order to achieve high performance. However, continuous high intensity training often results in injuries to the locomotive system. The buoyancy of water reduces the force born on joints; therefore, training in water has been used for rehabilitation purposes both in humans and horses. The few studies dealing with water treadmill training of horses suggest that the fitness of horses can be improved using this training method, but none tested the subsequent performance of horses after water training. Therefore, the aim of this study was to test the effect of water training of varying...
Distribution of antiseptic resistance genes in Staphylococcus spp. from bovine mastitisOriginal Paper
Y. Ergun, Z. Cantekin, K. Gurturk, H. Solmaz, I.H. Ekin, D. Ozturk
Vet Med - Czech, 2017, 62(4):200-203 | DOI: 10.17221/265/2015-VETMED
The purpose of this study was the determination of antiseptic resistance genes (qacA/B and qacC) from staphylococcal mastitis in cattle in various regions of Turkey. In total, 283 isolates (Burdur: 36, Hatay: 47 and Van: 200) were studied, and the antiseptic resistance genes were detected using simplex PCR. The distribution of the qacA/B and qacC genes, mediating resistance against quaternary ammonium compounds, was found to vary among the different isolates. The qacA/B genes were found in three of the Burdur isolates, six of the Hatay isolates and seven of the Van isolates. The...
Determination of sex-related differences based on 3D reconstruction of the chinchilla (Chinchilla lanigera) vertebral column from MDCT scansOriginal Paper
S. Ozkadif, E. Eken, M.O. Dayan, K. Besoluk
Vet Med - Czech, 2017, 62(4):204-210 | DOI: 10.17221/19/2015-VETMED
This study was undertaken to obtain and analyse, on the basis of sex, three-dimensional (3D) reconstructions obtained by a 3D computer program from two-dimensional (2D) vertebral column sections taken by multidetector computed tomography (MDCT) images, in the chinchilla. A total of 16 adult chinchillas (Chinchilla lanigera) of both sexes were used. The MDCT images were taken under general anaesthesia, and were then transferred to a personal computer on which 3D reconstructions were carried out using a 3D modelling program (Mimics 13.1). The volume, surface area and vertebral body length of each vertebra (except caudal region) forming the vertebral...
Multiplex real-time reverse transcription polymerase chain reaction for differential detection of H5, N1, and N8 genes of highly pathogenic avian influenza virusesOriginal Paper
Y.R. Park, E.M. Kim, Y.J. Lee, S.G. Yeo, C.K. Park
Vet Med - Czech, 2017, 62(4):211-220 | DOI: 10.17221/179/2016-VETMED
Rapid and differential diagnosis of highly pathogenic avian influenza virus (HPAIV) subtype H5 is essential for the effective prevention and control of outbreaks caused by this pathogen. In this study, we describe a one-step multiplex real-time reverse transcription polymerase chain reaction (mRRT-PCR), using H5-, N1-, and N8-specific primers and probes, for differential detection of two HPAIVs (H5N1 and H5N8) and other H5-subtype AIVs. Using the mRRT-PCR assay, we were able to detect H5N1, H5N8, and other H5-subtype AIVs in a one-tube reaction, with high specificity; furthermore, using an in silico PCR program, we confirmed that this assay...
The Cys83Gly amino acid substitution in feather keratin is associated with pigeon performance in long-distance racesOriginal Paper
W.S. Proskura, A. Lukaszewicz, E. Dzierzba, D. Cichon, D. Zaborski, W. Grzesiak, A. Dybus
Vet Med - Czech, 2017, 62(4):221-225 | DOI: 10.17221/271/2015-VETMED
The aim of this study was to investigate the association of the g.710T>G polymorphism in the keratin gene, which results in a cysteine to glycine amino acid change at position 83 (Cys83Gly) in feather keratin, with homing pigeon racing performance. A total of 123 homing pigeons were investigated. The data set used in this study consisted of scores from 17 short races (less than 400 km) and 11 long races (greater than 500 km) that took place in the 2011 and 2012 racing seasons (2589 race records in total). The genotyping of the g.710T>G polymorphism was performed using the artificially created restriction site-PCR assay. The T allele...
Bifid phallus with complete duplication and a separate scrotum in a German shepherd dog: a case reportCase Report
M. Karabagli, B. Karan, U. Ugurlu, Z. Mutlu, F. Yildirim, I. Firat, A. Baran, A. Ucur, A. Bayrak, S. Ozturk
Vet Med - Czech, 2017, 62(4):226-230 | DOI: 10.17221/16/2017-VETMED
Diphallia or penile duplication is a rare congenital abnormality thought to result from duplication of the cloacal membrane in the early developmental stages in the uterus. The present case concerned a 4-year old intact male German shepherd dog. The patient presented with a complaint of paraphimosis. Clinical, laboratory, radiological and ultrasonographic examinations were carried out. Semen samples collected prior to surgery were examined. Following amputation, the penis with paraphimosis was sent for histopathological investigation. Karyotype analysis of the patient was also performed. As well as the first report of this abnormality in Turkey, this...
Fish death caused by gas bubble disease: a case reportCase Report
J. Machova, R. Faina, T. Randak, O. Valentova, C. Steinbach, H. Kocour Kroupova, Z. Svobodova
Vet Med - Czech, 2017, 62(4):231-237 | DOI: 10.17221/153/2016-VETMED
This work summarises the findings of an investigation on a local trout farm (Czech Republic), which was carried out in connection with the repeated deaths of salmonids (brook trout, Salvelinus fontinalis and rainbow trout, Oncorhynchus mykiss). These fishes were reared in newly installed tanks that were supplied with water from the same source as the original outdoor nature pond where the fishes had been reared without problems. The skin of dead fish was pale and covered with a thin layer of mucus. The gills had lighter colour, and microscopically, gas bubbles were visible both on the surface of gills and inside the gill filaments....