Title:Evaluation of the Antioxidant and Antimicrobial Properties of Hydrosol Extract and Essential oil Derived from the Aerial Parts of Salvia argentea
Volume: 23
Issue: 2
Author(s): Ilyas Chikhi, Rachida Kerzabi, Safaa Nor el Houda Ziane, Fatima Benyoucef, Mohammed El Amine DIB*Alain Muselli
Affiliation:
- Laboratoire des Substances
Naturelles et Bioactives (LASNABIO), Université Abou bekr Belkaid, Tlemcen, 13000 Algérie
Keywords:
Hydrosol extract, Essential oil, antimicrobial activities, antioxidant activities, agar dicc diffusion method, gas chromatography.
Abstract:
Background: The increasing use of synthetic antimicrobials and antioxidants is a concern
for human health.
Aims: The main objective of this study was to examine the chemical composition of the Essential
oil extracted from Salvia argentea and its hydrosol extract while evaluating their antioxidant and
antimicrobial effects in vitro.
Materials and Methods: The Essential oil was obtained by hydrodistillation, while the hydrosol
extract was obtained by the liquid-liquid extraction method. The volatile compounds of the Essential
oil and hydrosol extract were analysed by gas chromatography (GC/FID) and gas chromatography
coupled with mass spectrometry (GC/MS). Antioxidant activities were evaluated using
two methods: free radical scavenging activity (DPPH) and iron reduction antioxidant power
(FRAP), with BHT used as a positive control. The antimicrobial activity of the Essential oil and
hydrosol extract was evaluated using the agar disc diffusion method.
Results: According to the GC/FID and GC/MS analysis, the Essential oil of S. argentea consisted
mainly of germacrene D-4-ol (26.3%), epi-α-muurolol (19.2%), and epi-α-cadinol (10.2%). In
contrast, the hydrosol extract consisted of oxygenated compounds, including (E)-phytol (23.5%),
14-hydroxy-δ-cadine (17.5%), caryophyllene oxide (11.5%), piperitone (13.5%), and piperitone
oxide (11.5%). The Essential oil and hydrosol extract showed positive antioxidant activity with
both tests. It should be noted that the hydrosol extract showed the best performance, with an IC50
of 8.3 g/L with the DPPH and an IC50 of 12.3 g/L with the FRAP test compared to the BHT. The
Essential oil revealed antimicrobial activity with strong inhibition against Escherichia coli,
Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis compared to Gentamicin.
Conclusion: The results showed that Essential oil and hydrosol extract have promising antimicrobial
and antioxidant properties. These findings provide encouraging prospects for their potential
use in the pharmaceutical or cosmetic sectors.
