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[(immunopeptidome,)-360.7(and)-354(its)-352.7(role)-353.5(in)-351.8(developing)-360.2(universal)-352.2(anti-viral)]TJ 0 -1.3921 TD [(and)-511.4(anti)-9.6(-ca)-8.7(ncer)-511.8(immu)-8.6(noth)-9.4(erapi)-12.4(es)-505(t)-6.7(hat)-506.4(coul)-8.5(d)-506.8(ser)-8.7(ve)-505.5(as)-509.2(safe)-507.7(an)-11(d)]TJ 0 -1.3858 TD [(ef)58.2(fective)-300.8(alternatives)-303.4(to)-300.4(current)-302.2(treatment)-309.5(options.)]TJ /F1 1 Tf 13.5 0 0 13.5 49.6063 190.2047 Tm 0.85098 0.19216 0.2 scn [(HLA-E)-302.8(expression)-303.8(patterns)-302.3(and)]TJ 0 -1.1507 TD [(stability)-302.9(in)-301.9(human)-305.8(tissues)-301.6(and)]TJ 0 -1.1465 TD [(cell)-302(types)]TJ /F6 1 Tf 9 0 0 9 63.6094 134.1921 Tm 0.15294 0.15294 0.15294 scn [(Non-classical)-404.2(HLA-E)-394.2(has)-400.3(signi)]TJ /F7 1 Tf 12.6362 0 TD ()Tj /F6 1 Tf 0.5291 0 TD [(cantly)-399.1(lower)-400.9(RNA)-397.8(expression)]TJ -14.7213 -1.3858 TD [(levels)-227.1(than)-223.6(classical)-226.4(HLA-A,)-227.5(-B,)-224.5(and)-228(-C.)-220.8(Speci)]TJ /F7 1 Tf 17.9402 0 TD ()Tj /F6 1 Tf 0.5291 0 TD [(cally,)-226.1(a)-224.4(study)-229.7(showed)]TJ -18.4693 -1.3921 TD [(that)-217(HLA-E)-224.1(transcripts)-219.5(exhibit)-223.6(the)-218.7(highest)-218.3(median)-226.6(expression)-221.6(in)-219.5(the)]TJ 0 -1.3858 TD [(blood)-394.5(\(275)-386.9(RPKM\),)-397(spleen)-390.6(\(187)-393.2(RPKM\),)-397(lung)-391.6(\(142)-393.2(RPKM\),)-390.7(and)]TJ 0 -1.3921 TD [(adi)-11.3(pos)-8.1(e)-503.7(t)-6.7(issu)-10.1(e)-510(\(14)-9.2(1)-503.7(R)0(P)-8.2(KM\))-512.3(wh)-7.7(erea)-11.1(s)-505.3(c)0(l)-8(assi)-11.2(ca)-10(l)-505.9(HLA-)-11.3(I)-504.8(mole)-12.5(cu)-8.9(les)]TJ 0 -1.3858 TD [(show)-504.4(the)-502.2(highe)-8.7(st)-499.3(median)-510(expression)-511.4(levels)-504.2(in)-502.9(the)-508.5(whole)-504.7(blood)]TJ 28.3654 76.3528 TD [(\(1210)-430.7(RPKM\),)-434.8(spleen)-441(\(850)-431(RPKM\),)-434.8(bone)-436.7(marrow)-436.5(\(615)-431(RPKM\),)]TJ 0 -1.3858 TD 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[(was)-299.9(g)-2.9(en)-8.1(erate)-6.7(d)-299.7(aut)-7.1(o)-1.4(mati)-5.8(cal)-3.9(l)-3.9(y)-296.5(u)-8.1(s)]TJ 13.6794 0 TD [(ing)-302.7(t)-7.2(he)-298.4(H)-3.2(u)-0.1(ma)-8.3(n)-299.8(P)1.8(rot)-7.2(e)1.3(in)-307.9(Atl)-4(a)-0.2(s)-300(\()]TJ 0.85098 0.19216 0.2 scn 12.8128 0 TD -0.0224 Tc [(https:)-6.8(/)6.2(/)-1.9(www.proteinatlas.org/)]TJ 0.49804 0.49804 0.49412 scn 12.7318 0 TD 0.2778 Tc (\)\()Tj 0.85098 0.19216 0.2 scn 0.818 0 TD -0.0194 Tc (20)Tj 0.49804 0.49804 0.49412 scn 1.142 0 TD -0.0218 Tc (\).)Tj /F1 1 Tf -60.3142 88.5316 TD 0 Tc [(Weitzen)-302.7(et)-300.8(al.)]TJ 57.0665 0 TD (10.3389/fimmu.2024.1442783)Tj -57.0665 -109.3302 TD [(Frontiers)-304.6(in)]TJ 0.85098 0.19216 0.2 scn 5.5074 0 TD (Immunology)Tj 58.8564 0 TD (frontiersin.org)Tj 0.15294 0.15294 0.15294 scn -29.5051 0 TD (03)Tj ET endstream endobj 44 0 obj<>stream Hӻk_sQQT&j#Zy h-x Z+v `NV7(V"&AD4s|]̻nvbL&;|3gY?6)#CJ%K)^ K)6yGHd!ٳg^ !C˗0)'Ns6h,zzzuH)1/L~ᦳ)R2L_JqarΜ9ۆP߿3gNclٲ,˪RJe(Ccbq̽{SJ&(ʕ+CM1LB__ٔRyPc 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[(n)4.9(i)1(ty)-531.7(in)-530.6(the)-529.6(e)-0.4(ndoplas)4.2(m)-1.3(ic)]TJ -15.9685 -1.3858 TD 0 Tc [(reticulum)-417.1(\(ER\))-418.3(system)-414.7(to)-413.7(be)-413.1(presented)-418.1(on)-414.1(the)-414(cell)-419.2(surface)-417.2(for)-413.5(T)]TJ 0 -1.3921 TD [(cell)-211.3(immunosurveillance)-222(\()]TJ 0.85098 0.19216 0.2 scn 10.2425 0 TD (59)Tj 0.15294 0.15294 0.15294 scn 0.9575 0 TD (,)Tj 0.85098 0.19216 0.2 scn 0.4346 0 TD (60)Tj 0.15294 0.15294 0.15294 scn 0.9575 0 TD [(\).)-207.5(TAP)-215.7(\(Transporter)-219(associated)-210.7(with)]TJ -12.5921 -1.3858 TD [(Antigen)-391.8(Processing\))-394.6(is)-390.2(a)-394.5(key)-388.2(player)-395.9(in)-389.5(antigen)-389.9(processing,)-397.9(which)]TJ 0 -1.3921 TD [(transports)-313.6(peptide)-315.3(fragments)-317.3(from)-313.3(the)-313.2(cytosol)-309.2(into)-311.9(the)-313.2(ER)-310.6(system.)]TJ 0 -1.3858 TD [(Upon)-431.2(TAP)-436.2(impairment,)-437.5(new)-432.1(peptides)-436.3(have)-432(been)-432.5(reported)-434.2(to)-432.6(be)]TJ 0 -1.3921 TD [(presented)-216.6(by)-213.6(HLA-E.)-214(Speci)]TJ /F7 1 Tf 10.6583 0 TD ()Tj /F6 1 Tf 0.5291 0 TD [(cally,)-213.5(in)-213.2(2015,)-215.6(Kraemer)-216.3(et)-214.3(al.)-210(used)-214.6(TAP-)]TJ -11.1874 -1.3858 TD (de)Tj /F7 1 Tf 0.9386 0 TD ()Tj /F6 1 Tf 0.5291 0 TD [(cient)-313.8(T2)-311.7(cell)-305.8(lines)-317(transduced)-311.8(with)-308.7(soluble)-311.8(HLA-E*01:01.)-318(They)]TJ -1.4677 -1.3921 TD [(found)-317.7(36)-314.4(HLA-E*01:01-bound)-322.2(peptides)-316.6(with)-315(27)-314.4(being)-319.5(longer)-318.2(than)]TJ 0 -1.3858 TD [(10)-333.3(amino)-341.3(acids)-333.7(\(11-16)-341.3(mers\))-334(and)-341.4(originating)-337.1(from)-338.5(various)-334.8(source)]TJ 0 -1.3921 TD [(proteins,)-370.1(including)-366.2(cell)-362.5(cycle)-362.1(regulatory)-365.6(proteins,)-370.1(matrix)-368(proteins,)]TJ 0 -1.3858 TD [(DNA)-371(repair)-379.1(proteins,)-376.4(and)-372.9(stress-induced)-376.6(proteins)-373.9(\()]TJ 0.85098 0.19216 0.2 scn 21.0835 0 TD (47)Tj 0.15294 0.15294 0.15294 scn 0.9575 0 TD [(\).)-371.3(However,)]TJ -22.0409 -1.3921 TD 0.0128 Tc [(those)-509.8(p)-2.6(eptides)-511.4(n)-0.5(eed)-512.9(f)-4.9(u)3.7(rthe)-5.8(r)-511.4(e)0.5(xperime)-5.8(n)-0.5(tal)-512(v)-1.6(alidation.)-512.5(In)-510.8(2)-5.8(013,)]TJ 0 -1.3858 TD 0 Tc [(another)-379.8(study)-380.9(demonstrated)-389.4(that)-374.4(the)-382.5(repertoire)-386.6(of)-374.4(HLA-E-bound)]TJ 0 -1.3921 TD [(peptides)-430(retained)-435.3(the)-426.6(binding)-428.6(motif)-430.3(of)-424.7(HLA-A*02:01)-429.9(upon)-426.1(TAP)]TJ 0 -1.3858 TD [(inhibition)-359.9(\()]TJ 0.85098 0.19216 0.2 scn 4.6614 0 TD (55)Tj 0.15294 0.15294 0.15294 scn 0.9575 0 TD [(\).)-358.7(Speci)]TJ /F7 1 Tf 2.9984 0 TD ()Tj /F6 1 Tf 0.5354 0 TD [(cally,)-358.4(the)-357.3(authors)-358.3(used)-359.5(TAP-de)]TJ /F7 1 Tf 12.7055 0 TD ()Tj /F6 1 Tf 0.5291 0 TD [(cient)-357.9(K562)]TJ -22.3874 -1.3921 TD 0.0153 Tc [(cells)-515.2(made)-513.6(by)-515.7(intr)-5(oducing)-515.7(t)2.3(he)-513.6(herpes)-515.2(virus)-515.2(e)3(vasion)-514.6(molecule)]TJ 0 -1.3858 TD 0 Tc [(UL49.5,)-300(which)-306.2(blocks)-303(TAP)-297.6(function)-307.7(and)-303.6(_targets)-298.7(it)-301(for)-306.4(proteasomal)]TJ 0 -1.3921 TD [(degradatio)-12.7(n.)-500.8(Despite)-507.9(reduced)-513(peptide)-504.2(transp)-10.6(ort,)-505.8(surface)-505.4(HLA-E)]TJ 0 -1.3858 TD 0.044 Tc [(levels)-543.2(rema)-4(ined)-544.7(unch)-4.5(anged,)-544.3(i)-4.7(n)-0.8(di)-4.7(cati)-4.7(ng)-543.7(stab)-4(iliz)-6.1(atio)-4(n)-542.6(b)-4(y)-543.7(a)2.3(n)]TJ 0 -1.3921 TD 0 Tc [(alternative,)-329.7(TAP-independent)-330.1(peptide)-327.9(repertoire)-329.9(\()]TJ 0.85098 0.19216 0.2 scn 20.1512 0 TD (55)Tj 0.15294 0.15294 0.15294 scn 0.9575 0 TD [(\).)-327.2(MS)-322.6(analysis)]TJ -21.1087 -1.3858 TD [(of)-386.9(HLA-E-binding)-386.9(peptides)-392.2(from)-388.9(K562)-385.2(cells)-382.7(revealed)-389.1(552)-389.8(unique)]TJ 0 -1.3921 TD [(peptide)-220.8(sequences)-219.6(in)-213.2(TAP-de)]TJ /F7 1 Tf 11.5276 0 TD ()Tj /F6 1 Tf 0.5291 0 TD [(cient)-219.3(cells)-212.6(\()]TJ 0.85098 0.19216 0.2 scn 4.4094 0 TD (55)Tj 0.15294 0.15294 0.15294 scn 0.9575 0 TD 0.2113 Tc (\)\()Tj 0.85098 0.19216 0.2 scn 0.9008 0 TD 0 Tc [(Supplementary)-218.5(Table)]TJ 10.0409 73.7071 TD 0.0422 Tc (S1)Tj 0.15294 0.15294 0.15294 scn 1.0331 0 TD 0.0459 Tc [(\),)-548.7(with)-550.7(only)-548.1(eight)-540.3(o)-2.1(ver)-5.8(l)6.1(apping)-541.8(with)-544.4(the)-546(T)-1.5(AP-pro)]TJ /F7 1 Tf 23.0551 0 TD 0 Tc ()Tj /F6 1 Tf 9 0 0 9 526.8471 757.1337 Tm 0.0501 Tc [(ci)7.7(en)5.3(t)]TJ -24.6614 -1.3921 TD 0 Tc [(counterpart,)-309.4(indicating)-307.3(a)-306.3(distinct)-310.6(peptide)-309(repertoire)-311(in)-307.7(the)-306.9(absence)]TJ 0 -1.3858 TD [(of)-449.9(TAP)-455.1(function)-458.9(\()]TJ 0.85098 0.19216 0.2 scn 7.7354 0 TD (55)Tj 0.15294 0.15294 0.15294 scn 0.9575 0 TD [(\).)-453.2(Interestingly,)-465.1(the)-451.8(majority)-459.5(of)-456.2(the)-451.8(HLA-E)]TJ -8.6929 -1.3921 TD [(peptide)-271.2(ligands)-275.5(were)-275(9-mers,)-281.2(and)-272.1(few)-272.7(peptides)-278.8(were)-275(longer)-274.1(than)-274(11)]TJ 0 -1.3858 TD [(amino)-221.6(acids.)-222.8(The)-221.7(peptide)-220.8(sources)-224.5(were)-224.6(diverse,)-228.1(and)-221.7(many)-221.3(did)-218.1(not)]TJ /F7 1 Tf 25.9528 0 TD ()Tj /F6 1 Tf 0.5291 0 TD (t)Tj -26.4819 -1.3921 TD 0.0351 Tc [(p)7.1(r)2.2(ev)8.1(io)6(usly)-527.4(kn)2.9(own)-526.3(p)0.8(ro)6(ce)3.9(ssi)5.3(ng)-527.4(m)3(e)3.9(ch)5.5(an)2.9(i)5.3(s)2.2(m)3(s)2.3(,)-534.3(s)2.2(ug)8.1(gest)3.2(i)5.3(n)2.9(g)-533.7(o)6(t)3.2(h)-0.8(e)3.9(r)]TJ 0 -1.3858 TD 0.0198 Tc [(p)4.4(a)-3(thw)3.9(a)-3(ys,)-518.1(s)5.8(uch)-520.1(a)3.3(s)-523.3(a)3.3(uto)3.3(p)-1.9(hag)5.4(y)-0.9(,)-518.1(m)]TJ 14.3433 0 TD [(ight)-515.9(contr)5.9(i)-3.6(bute)-515.3(to)-519.5(the)-521.6(T)4(AP-)]TJ -14.3433 -1.3921 TD 0 Tc [(independent)-430.6(peptide)-428.7(repertoire)-430.7(\()]TJ 0.85098 0.19216 0.2 scn 13.4992 0 TD (55)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD [(\).)-421.7(Voogd)-430.5(et)-422.2(al.)-430.4(also)-426.9(described)]TJ -14.463 -1.3858 TD [(potential)-434.2(TAP-independent)-449.8(routes)-437.9(facilitating)-441.1(peptide)-441.3(processing)]TJ 0 -1.3921 TD 0.0135 Tc [(an)-6.1(d)-512.2(p)-8.2(res)-6.8(e)1.2(nt)-5.8(ation)-516.4(o)-3(nt)-5.8(o)-513.3(H)-1.3(LA)-5(-E)-520.6(molec)-5.2(u)-1.9(les)-517(\()]TJ 0.85098 0.19216 0.2 scn 18.5764 0 TD 0.0186 Tc (15)Tj 0.15294 0.15294 0.15294 scn 0.989 0 TD 0.0153 Tc [(\).)-516.3(An)-514.6(alternative)]TJ -19.5654 -1.3858 TD 0.0169 Tc [(pathway)-514.1(i)-0.3(nvolves)-513.6(f)-0.8(u)-4.8(r)2.9(in,)-514.7(a)-516.2(p)-4.8(r)2.9(otease)-518.3(in)-519.3(the)-518.3(t)-2.4(rans-)5.5(G)-5.8(olgi,)-521(w)1(hich)]TJ 0 -1.3921 TD 0.0261 Tc [(exhibits)-529.6(endoprote)-5.1(o)3.3(lytic)-528(a)-3(ctivity)-530.1(b)3.3(y)-530.1(c)1.2(leaving)-530.1(p)-1.9(ro-proteins)-523.3(a)-3(t)]TJ 0 -1.3858 TD 0 Tc [(designated)-358.1(C-terminal)-357.6(cleavage)-359.2(sites)-360.1(\()]TJ 0.85098 0.19216 0.2 scn 15.3953 0 TD (15)Tj 0.15294 0.15294 0.15294 scn 0.9575 0 TD [(\).)-358.7(Therefore,)-361.3(despite)-357.9(TAP)]TJ -16.3528 -1.3921 TD [(impairment,)-261.1(HLA-E)-261.9(can)-256.4(present)-264.4(diverse)-263.4(arrays)-259.4(of)-261(peptides)-266.2(through)]TJ 0 -1.3858 TD [(alternative)-365(pathways.)-370.9(The)-372.8(lengths)-369.5(and)-366.6(identities)-371.6(of)-368.1(these)-364.7(peptides)]TJ 0 -1.3921 TD [(vary)-374.3(across)-383.3(dif)63.1(ferent)-384.9(studies.)-380.9(In)-379.5(fact,)-383.2(a)-375.6(comprehensive)-385.9(analysis)-378.7(of)]TJ 0 -1.3858 TD [(HLA-E-bound)-312.7(peptides)-322.9(from)-313.3(multiple)-317.2(independent)-317.2(MS-based)-320.5(and)]TJ 0 -1.3921 TD [(non-MS)-301.5(studies)-309.1(reveals)-306(that)-305.2(these)-301.7(peptides)-310.3(are)-301.1(generally)-309.2(unique)-307(to)]TJ 0 -1.3858 TD [(each)-448.8(study,)-458.9(with)-447.3(minimal)-457.9(overlap)-450.2(\()]TJ 0.85098 0.19216 0.2 scn 14.8787 0 TD [(Figure)-454.1(2)]TJ 0.15294 0.15294 0.15294 scn 3.9244 0 TD (and)Tj 0.85098 0.19216 0.2 scn 1.9528 0 TD (Supplementary)Tj -20.7559 -1.3858 TD [(Table)-365.7(S1)]TJ 0.15294 0.15294 0.15294 scn 3.5276 0 TD [(\).)-365(Four)-370(peptides)-373.3(were)-363.2(found)-368.1(to)-363.3(be)-369(common)-368.6(between)-366.6(MS)]TJ -3.5276 -1.3921 TD [(and)-272.1(non-MS)-270(studies)-277.6(\()]TJ 0.85098 0.19216 0.2 scn 8.6992 0 TD [(Figure)-271.4(2A)]TJ 0.15294 0.15294 0.15294 scn 3.9748 0 TD [(\).)-276.8(All)-268.1(peptides)-278.8(were)-275(9-mers)-272.4(in)-269.9(non-)]TJ -12.674 -1.3858 TD [(MS)-196.6(studies)-208.3(whereas)-202.9(peptide)-208.2(lengths)-199.4(were)-205.7(more)-200.9(variable)-204(in)-200.6(MS-based)]TJ 0 -1.3921 TD [(studies)-510.7(\()]TJ 0.85098 0.19216 0.2 scn 3.5969 0 TD [(Fig)-9.2(ure)-501.6(2B)]TJ 0.15294 0.15294 0.15294 scn 4.1134 0 TD [(\).)-503.6(Predic)-13.2(ted)-506.9(binding)-510.5(af)]TJ /F7 1 Tf 9.6189 0 TD ()Tj /F6 1 Tf 0.5291 0 TD [(nity)-505.1(to)-501.9(HLA-)-11.3(E)-496.3(usi)-8.7(ng)]TJ -17.8583 -1.3858 TD [(NetMHCpan)-331.4(4.1)-335.8(indicates)-332.8(a)-331.5(~2-fold)-334.8(increase)-338.7(in)-332.9(the)-332.1(proportion)-334.5(of)]TJ 0 -1.3921 TD 0.0141 Tc [(non)-5.5(-)2.7(binder)-6.2(s)-510.1(\()-2(NB\))-518.5(i)3.2(n)-515.8(M)-1.7(S-based)-517.9(st)-5.2(udie)-4.5(s)-510.1(c)-4.5(o)-2.4(mpared)-517.9(to)-519(non-MS)]TJ 0 -1.3858 TD 0 Tc [(studies)-328(\()]TJ 0.85098 0.19216 0.2 scn 3.4142 0 TD [(Figure)-328.1(2C)]TJ 0.15294 0.15294 0.15294 scn 4.0063 0 TD [(\).)-320.9(These)-329.8(predictions)-330.7(should)-326(be)-324.9(approached)-334.2(with)]TJ -7.4205 -1.3921 TD [(caution)-488.5(since)-484.9(prediction)-493.1(algorithms)-490.5(for)-489.1(HLA-E)-488.7(ligands)-489.7(are)-483.8(still)]TJ 0 -1.3858 TD [(relatively)-269.3(sub-optimal)-270.7(in)-269.9(comparison)-274.9(with)-270.9(classical)-270.5(HLA-ABC,)-273.6(and)]TJ 0 -1.3921 TD [(more)-301.7(data)-307.1(are)-307.4(required)-315(to)-300.4(improve)-310.9(their)-306.5(performances.)-312.6(Additional)]TJ 0 -1.3858 TD 0.0136 Tc [(compar)-6.7(isons)-516.9(b)-2.9(etwee)-5(n)-516.3(seve)-5(ral)-511.2(s)]TJ 13.3354 0 TD 0.017 Tc [(tud)7.9(i)-0.2(e)4.7(s)-513.5(s)3(h)0(ow)-509.2(lim)3.8(i)6.1(te)4.7(d)-515(o)6.8(ver)3(l)2.4(ap)-508.7(in)]TJ -13.3354 -1.3921 TD 0 Tc [(co)-10(m)-13.2(m)-6.9(on)-521.2(H)-8.5(L)-10.7(A-)-17.3(E)-515.2(p)-9.1(ep)-15.2(t)-6.7(i)-10.9(d)-9.1(e)0(s)-523.9(\()]TJ 0.85098 0.19216 0.2 scn 11.8173 0 TD 0.0088 Tc [(Figure)8.8(s)-515.1(2)8.8(D)]TJ /Cs6 cs 0 0 0 scn 4.6929 0 TD 0 Tc (,)Tj /Cs6 cs 0.85098 0.19216 0.2 scn 0.737 0 TD (E)Tj 0.15294 0.15294 0.15294 scn 0.5669 0 TD [(\))-9.8(.)-519(Th)-14.1(u)-9.1(s)-7.7(,)-519(o)0(u)-13.1(r)-517.9(an)-17.3(al)-12.2(y)-8.1(s)-7.7(i)-10.9(s)]TJ -17.8142 -1.3858 TD [(unde)-12.4(rs)-9(core)-11.1(s)-505.3(t)-6.7(he)-508.1(nece)-12.6(ss)-9(ity)-510.7(fo)-9(r)-505.3(f)0(u)-7.9(r)0(t)-8(her)-509.5(inv)-13.4(est)-7.8(iga)-10.3(tio)-8.9(ns)-512.3(to)-508.2(enh)-11.2(anc)-10.7(e)]TJ 0 -1.3921 TD 0.0315 Tc [(our)-536.8(u)3.5(nderst)5.9(anding)-531(of)-534.2(t)5.9(h)-4.4(e)-528.9(H)-2.2(LA-E)-534.1(immunopeptidome)-535.2(u)3.5(nder)]TJ 0 -1.3858 TD 0 Tc [(various)-385.2(conditions,)-390.6(including)-391.4(TAP)-392.1(perturbations,)-388.8(viral)-391.6(infections,)]TJ 0 -1.3921 TD [(and)-297.3(oncogenic)-304.7(processes.)]TJ 1.5496 -1.3858 TD [(In)-379.5(this)-369.8(regard,)-378.9(tumor)-374.4(cells)-376.4(were)-375.8(shown)-372.8(to)-375.9(downregulate)-375.3(TAP)]TJ -1.5496 -1.3921 TD [(subunits)-320.3(to)-319.3(avert)-315.9(T-cell)-320.4(clearance)-325.9(\()]TJ 0.85098 0.19216 0.2 scn 14.085 0 TD (61)Tj 0.15294 0.15294 0.15294 scn 0.9575 0 TD [(\).)-320.9(In)-316.6(cancer,)-322.7(HLA-E)-318.6(peptides)]TJ -15.0425 -1.3858 TD [(may)-283.5(take)-289.8(advantage)-283.7(of)-286.2(non-classical)-293.6(TAP-independent)-292.3(pathways)-286.6(if)]TJ 0 -1.3921 TD [(the)-313.2(classical)-314.6(ER-based,)-317.3(TAP-dependent)-324.8(route)-310.6(is)-314.6(unavailable.)-321.6(It)-309.9(has)]TJ 0 -1.3858 TD [(been)-514.4(foun)-12.6(d)-506.8(tha)-8.7(t)-504.3(m)0(u)-9.7(rine)-510.3(TA)-9.2(P)]TJ 6.7499 0 0 5.9992 418.9605 273.6 Tm (-/-)Tj 9 0 0 9 430.5259 269.6315 Tm [(T-c)-8.1(el)-8(l)-499.6(l)-8.3(ymph)-9.7(oma)-512.4(cel)-7.8(ls)-513.6(pre)-10.2(sent)]TJ -13.9591 -1.3921 TD [(immunogenic)-334.8(alternative)-333.5(peptides)-335.5(on)-325.9(the)-332.1(mouse)-329.7(HLA-E)-337.5(ortholog,)]TJ 0 -1.3858 TD [(Qa1,)-312.8(and)-316.2(CD8)]TJ 6.7499 0 0 5.9992 357.6755 248.5984 Tm (+)Tj 9 0 0 9 364.3653 244.6299 Tm [(T-cell)-314.1(clone)-321.1(production)-319.2(was)-311(restricted)-320.1(\()]TJ 0.85098 0.19216 0.2 scn 16 0 TD (15)Tj 0.15294 0.15294 0.15294 scn 0.9575 0 TD [(\).)-314.6(These)]TJ -23.5654 -1.3921 TD [(alternative)-396.5(peptides)-404.8(are)-395.6(unique)-401.5(and)-398.1(have)-400.5(little)-393.2(overlap)-399.9(with)-396.9(their)]TJ 0 -1.3858 TD [(TA)-15.5(P-)-16.3(p)-9.1(r)-7.7(o)]TJ /F7 1 Tf 3.6598 0 TD ()Tj /F6 1 Tf 0.5354 0 TD 0.0115 Tc [(ci)11.5(en)4.5(t)-505.4(c)11.5(oun)4.5(t)4.8(erpart)4.8(s)-506.4(\()]TJ 0.85098 0.19216 0.2 scn 8.4976 0 TD 0 Tc (55)Tj 0.15294 0.15294 0.15294 scn 0.9701 0 TD [(\))-9.8(.)-519(On)-17.6(e)-516.3(a)-10.2(l)-8.3(t)-6.7(er)-13.7(n)-13.3(a)0(t)-16.9(i)0(v)-12.7(e)-516.3(p)-9.1(e)-12.3(p)-9.1(t)-6.7(id)-13.7(e)-516.3(w)-9.6(a)-10.2(s)]TJ -13.663 -1.3921 TD [(discovered)-458.9(from)-464.5(MS-based)-465.4(immunopeptidomic)-463.6(procedures:)-465.4(a)-463.8(9)0(-)]TJ 0 -1.3858 TD [(mer)-228.5(epitope)-228.2(named)-228.3(M-SL9)-231(\(SLQGRTLIL\))-229.2(associated)-235.9(with)-226.8(in)]TJ /F7 1 Tf 23.9181 0 TD ()Tj /F6 1 Tf 0.5354 0 TD (uenza)Tj -24.4535 -1.3921 TD 0.0114 Tc [(A)-504.8(v)-3(i)11.4(r)-7.1(us)-512.8(\(I)4.2(AV\))-508.6(P)0.2(ue)11.4(r)-8.6(t)4.7(o)-509.1(R)1.8(ico/8)11.4(/)-6.5(193)11.4(4)-517.2(\()1.6(PR8\))-508.6(wit)4.7(h)0.7(in)-512.2(I)4.2(A)-0.8(V-inf)11.4(e)-6(ct)4.7(ed)]TJ 0 -1.3858 TD 0.101 Tc [(C5)-5.8(7BL)-4.2(/)0.9(6)-610.6(m)-0.4(i)-4.4(c)0.5(e)-604.3(\()]TJ 0.85098 0.19216 0.2 scn 8.2205 0 TD 0.1041 Tc [(Table)-601.2(1)]TJ 0.15294 0.15294 0.15294 scn 3.9244 0 TD 0.1053 Tc [(\).)-602.7(Th)6.4(is)-601.6(epi)6.2(t)-2.2(o)6.9(p)-4.6(e)-600(e)4.8(lici)6.2(ts)-601.6(a)-597.9(c)-1.5(o-)]TJ -12.1449 -1.3921 TD 0.0272 Tc [(im)-4.9(mu)-7.1(nodom)-4.9(i)-2.6(nan)-5(t)1.6(,)-529.6(u)-7.1(nc)-4.1(onve)-4(n)-5(t)1.6(ional)-535.4(c)2.3(y)-6.1(t)1.6(olyt)-4.7(ic)-526.9(T)-7.6(-)3.2(c)-4(e)-4(l)0(l)-529.1(r)0.6(e)-4(s)-5.7(pons)-5.6(e,)]TJ 0 -1.3858 TD 0.0105 Tc [(uniquely)-514.2(prese)10.5(n)-8.9(te)10.5(d)-521.2(b)0.3(y)-507.9(Q)-0.1(a-1)]TJ /F8 1 Tf 12.0756 0 TD 0 Tc ()Tj /F6 1 Tf 0.5165 0 TD 0.0111 Tc 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694.375 re f 1 1 1 scn 532.233 760.309 10.261 4.989 re f 532.233 760.308 10.261 14.967 re f 532.233 70.923 10.261 4.989 re f 532.233 60.888 10.261 15.024 re f 532.233 60.888 0.73706 714.387 re f BT /F5 1 Tf 0 6.9999 -6.9999 0 54.7653 70.6394 Tm 0.49804 0.49804 0.49412 scn 0 Tc 0 Tw [(TABLE)-306.1(1)-802.1(A)-296.4(representative)-305.7(list)-305.5(of)-295.5(known)-304.8(HLA-E-associated)-304.6(epitopes)-306(originating)-304.2(from)-300.4(various)-298.9(protein)-304.5(sources)]TJ /F12 1 Tf 51.0893 0.2835 TD ()Tj /F5 1 Tf 0.4374 -0.2835 TD (.)Tj /F1 1 Tf 0 9 -9 0 76.8756 75.7418 Tm 1 1 1 scn (Peptide)Tj 0 -1.115 TD (Sequence)Tj 8.6488 0.5606 TD [(Length)-5119.9(Material)]TJ 23.5402 0.5543 TD (Immunopeptidome)Tj 0 -1.115 TD [(Enrichment)-300.8(Method)]TJ 10.2866 0.5606 TD [(MS)-302.1(Method)]TJ 8.1197 0.5543 TD [(HLA-E)-302.8(peptide)-303.5(recogni-)]TJ 0 -1.115 TD [(tion)-303.9(by)-299.1(T-cell)-302.9(assay)]TJ 13.1339 0.5606 TD [(Origin)-7993.6(Ref)]TJ /F6 1 Tf 0 6.9999 -6.9999 0 223.0865 75.7418 Tm 0.15294 0.15294 0.15294 scn [(VMAPRTLVL)-5302.3(9)]TJ 21.811 16.4493 TD [(Dif)57(ferent)-302.3(cancer)-301.5(cell)-304.1(lines)-9362(N/A)]TJ /F13 1 Tf 21.325 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 0.6722 TD (NK)Tj /F14 1 Tf 1.4092 0 TD ()Tj /F6 1 Tf 0.575 0 TD [(CTL)-296.7(or)-302.3(HLA-A2-CTL-based)-308.9(T)]TJ -1.9843 -1.3526 TD [(cell)-304.1(assays)]TJ 16.8867 -4.7461 TD [(Human)-299.3(cytomegalovirus)]TJ 0 -1.3526 TD [(strains,)-300.6(UL40)-300.9(protein)]TJ 13.9305 6.779 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (28)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -75.3624 -3.0534 TD [(Murine)-302.3(TAP2-de)]TJ /F7 1 Tf 6.9248 0 TD ()Tj /F6 1 Tf 0.5345 0 TD [(cient)-300.4(RMA-S/HLA-E)-304.1(cell)]TJ -7.4593 -1.3607 TD [(line)-298.6(&)-304.4(Human)]TJ /F7 1 Tf 6.1472 0 TD ()Tj /F6 1 Tf 0.5345 0 TD [(broblast)-298.1(cell)-304.1(line)-306.7(HEK-293T)]TJ 12.8938 0.6803 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 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[(transporter)-304.6(multidrug)]TJ 0 -1.3526 TD [(resistance-associated)-311(protein)]TJ 13.9305 1.3526 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (33)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -75.3624 -6.4388 TD [(LCL)-298.7(721.221)-301.6(derivative)-304.5(cells)-304.5(transfected)-304.3(with)]TJ 0 -1.3607 TD [(HLA-Cw0401,)-299.8(-Cw1502,)-309.7(-G1,)-298.2(-Gs,)-299.8(and)-300(LCL)]TJ 19.5756 2.7213 TD [(Immunoprecipitation)-309.5(by)]TJ 0 -1.3607 TD [(anti-HLA)-299.1(class-I-pan)-303.3(W6/32)]TJ T* [(mAb)-301.2(crosslinked)-302.7(to)-299.5(protein)]TJ 0 -1.3526 TD [(A-Sepharose)-304.8(beads)]TJ 13.2259 2.7132 TD [(Finnigan)-305.4(TSQ)-301(7000)]TJ 0 -1.3607 TD [(LC-MS/MS)-306.8(system)]TJ 10.4398 0.6803 TD [(HLA-E)-305.2(peptide)-301.9(binding)-305.5(assay)]TJ 16.8867 0.6803 TD [(HLA-E)-297.1(and)-308.1(AEH)-299.3(proteins,)]TJ 0 -1.3607 TD [(expressed)-307(in)-300.5(721.221)-301.6(cells)]TJ 13.9305 0.6803 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (34)Tj 0.15294 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[(-Recombinant)-300.5(protein)-304.3(facility)]TJ 0 -1.3607 TD [(of)-302.4(SFR26)-299.1(\(synthetic)-303(peptides\))]TJ 13.9305 2.041 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (35)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 -13.2259 TD [(VMAPRTLIL)-5666.3(9)]TJ 21.811 8.8118 TD [(UL40-transfected)-307.9(cell)-304.1(line)-298.6(721.221)]TJ 19.5756 -5.5884 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 0 TD (NK)Tj /F14 1 Tf 1.4092 0 TD ()Tj /F6 1 Tf 0.575 0 TD [(CTL-based)-297.5(T)-301.2(cell)-304.1(assay)]TJ 14.9024 1.3526 TD [(Human)-299.3(cytomegalovirus)]TJ 0 -1.3526 TD [(strains,)-300.6(glycoprotein)]TJ 0 -1.3606 TD [(UL40)-300.9(protein)]TJ 13.9305 6.949 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (36)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -75.3624 -2.3649 TD [(HCMV-derived)-303.1(PBMCs)]TJ 74.0585 0 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (30)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -75.3624 -2.373 TD [(CMV-derived)-309.8(PBMCs)]TJ 74.0585 0 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (31)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -75.3624 -2.373 TD [(The)-301.8(murine)-302.4(TAP2-de)]TJ /F7 1 Tf 8.7147 0 TD ()Tj /F6 1 Tf 0.5345 0 TD [(cient)-300.4(T)-301.2(cell)]TJ -9.2492 -1.3607 TD [(lymphoma)-304.7(and)-300(RMA-S,)-305.3(cell)-304.1(line)]TJ 0 -1.3526 TD [(cotransfected)-303.5(with)-303.4(human)]TJ /F11 1 Tf 10.8042 0 TD (b)Tj /F6 1 Tf 0.5426 0 TD [(2m)-304.5(and)]TJ -11.3469 -1.3607 TD (HLA-E*01033)Tj 74.0585 2.041 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (37)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -75.3624 -5.7666 TD [(LCL)-298.7(721.221)-301.6(derivative)-304.5(cells)-304.5(transfected)-304.3(with)]TJ 0 -1.3607 TD [(HLA-Cw0401,)-299.8(-Cw1502,)-309.7(-G1,)-298.2(-Gs,)-299.8(and)-300(LCL)]TJ 19.5756 2.7213 TD [(Immunoprecipitation)-309.5(by)]TJ 0 -1.3607 TD [(anti-HLA)-299.1(class-I-pan)-303.3(W6/32)]TJ T* [(mAb)-301.2(crosslinked)-302.7(to)-299.5(protein)]TJ 0 -1.3526 TD [(A-Sepharose)-304.8(beads)]TJ 13.2259 2.7132 TD [(Finnigan)-305.4(TSQ)-301(7000)]TJ 0 -1.3607 TD [(LC-MS/MS)-306.8(system)]TJ 10.4398 0.6803 TD [(HLA-E)-305.2(peptide)-301.9(binding)-305.5(assay)]TJ 16.8867 0.6803 TD [(HLA-E)-297.1(and)-308.1(AEH)-299.3(proteins,)]TJ 0 -1.3607 TD [(expressed)-307(in)-300.5(721.221)-301.6(cells)]TJ 13.9305 0.6803 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (34)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 -6.2687 TD [(VMAPRTLLL)-5468.3(9)]TJ 21.811 1.8628 TD [(Murine)-302.3(TAP2-de)]TJ /F7 1 Tf 6.9248 0 TD ()Tj /F6 1 Tf 0.5345 0 TD [(cient)-300.4(RMA-S/HLA-E)-304.1(cell)]TJ -7.4593 -1.3607 TD [(line)-298.6(&)-304.4(Human)]TJ /F7 1 Tf 6.1472 0 TD ()Tj /F6 1 Tf 0.5345 0 TD [(broblast)-298.1(cell)-304.1(line)-306.7(HEK-293T)]TJ 12.8938 -0.5021 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 0 TD (NK)Tj /F14 1 Tf 1.4092 0 TD ()Tj /F6 1 Tf 0.575 0 TD [(CTL-based)-297.5(T)-301.2(cell)-304.1(assay)]TJ 14.9024 0.6803 TD [(Human)-299.3(cytomegalovirus)]TJ 0 -1.3607 TD [(strains,)-300.6(UL40)-300.9(protein)]TJ 13.9305 1.8628 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (29)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -75.3624 -3.0453 TD [(CMV-derived)-309.8(PBMCs)]TJ 74.0585 0 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (31)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj /F15 1 Tf -3.4745 -2.3002 TD (\(Continued\))Tj /F1 1 Tf 6.9999 0 0 6.9999 49.6063 800.9573 Tm 0.49804 0.49804 0.49412 scn [(Weitzen)-302.7(et)-300.8(al.)]TJ 57.0665 0 TD (10.3389/fimmu.2024.1442783)Tj -57.0665 -109.3302 TD [(Frontiers)-304.6(in)]TJ 0.85098 0.19216 0.2 scn 5.5074 0 TD (Immunology)Tj 58.8564 0 TD (frontiersin.org)Tj 0.15294 0.15294 0.15294 scn -29.5051 0 TD (05)Tj ET endstream endobj 103 0 obj<>stream Hbd`ad`ddpsvL) 1JHN51M62If!#[կ?~W&K9(3=DA#YSB!R+X4,5/=QO1'GX(8,5E2 ($} |;滐o? 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f 513.014 760.308 10.205 14.967 re f 513.014 70.923 10.205 4.989 re f 513.014 60.888 10.205 15.024 re f 513.014 60.888 0.737 714.387 re f BT /F5 1 Tf 0 6.9999 -6.9999 0 54.7653 70.6961 Tm 0.49804 0.49804 0.49412 scn 0 Tc 0 Tw [(TABLE)-298(1)-802.1(Continued)]TJ /F1 1 Tf 0 9 -9 0 76.7055 75.7418 Tm 1 1 1 scn (Peptide)Tj 0 -1.1087 TD (Sequence)Tj 8.6488 0.5543 TD [(Length)-5119.9(Material)]TJ 23.5402 0.5543 TD (Immunopeptidome)Tj 0 -1.1087 TD [(Enrichment)-300.8(Method)]TJ 10.2866 0.5543 TD [(MS)-302.1(Method)]TJ 8.1197 0.5543 TD [(HLA-E)-302.8(peptide)-303.5(recogni-)]TJ 0 -1.1087 TD [(tion)-303.9(by)-299.1(T-cell)-302.9(assay)]TJ 13.1339 0.5543 TD [(Origin)-7993.6(Ref)]TJ /F6 1 Tf 0 6.9999 -6.9999 0 103.011 228.4157 Tm 0.15294 0.15294 0.15294 scn [(Murine)-302.3(TAP-2-de)]TJ /F7 1 Tf 7.2811 0 TD ()Tj /F6 1 Tf 0.5264 0 TD [(cient)-308.5(RMA-S)-296.5(cells)]TJ 66.2509 0 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (38)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -75.3624 -3.7256 TD [(LCL)-298.7(721.221)-301.6(derivative)-304.5(cells)-304.5(transfected)-304.3(with)]TJ 0 -1.3607 TD [(HLA-Cw0401,)-299.8(-Cw1502,)-309.7(-G1,)-298.2(-Gs,)-299.8(and)-300(LCL)]TJ 19.5756 2.7213 TD [(Immunoprecipitation)-309.5(by)]TJ 0 -1.3607 TD [(anti-HLA)-299.1(class-I-pan)-303.3(W6/32)]TJ T* [(mAb)-301.2(crosslinked)-302.7(to)-299.5(protein)]TJ 0 -1.3526 TD [(A-Sepharose)-304.8(beads)]TJ 13.2259 2.7132 TD [(Finnigan)-305.4(TSQ)-301(7000)]TJ 0 -1.3607 TD [(LC-MS/MS)-306.8(system)]TJ 10.4398 0.6803 TD [(HLA-E)-305.2(peptide)-301.9(binding)-305.5(assay)]TJ 16.8867 0.6803 TD [(HLA-E)-297.1(and)-308.1(AEH)-299.3(proteins,)]TJ 0 -1.3607 TD [(expressed)-307(in)-300.5(721.221)-301.6(cells)]TJ 13.9305 0.6803 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (34)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -75.3624 -4.4059 TD [(-PBMCs)-305.5(derived)-301.9(from)-300.8(blood)-305.6(sample)-303(of)-294.3(a)]TJ 0 -1.3526 TD [(CMV-seropositive)-305.5(kidney-transplant)-306.8(patient)]TJ 0 -1.3607 TD [(-HLA-E-transfected)-306.5(\(721.221-E\))-303.3(and)-308.1(un-)]TJ T* [(transfected)-304.3(\(721.221\))-304.1(B-EBV)-306.8(cell)-296(lines)]TJ 19.5756 2.041 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 1.3526 TD [(-Antibody)-308.3(blocking)-295.5(and)-308.1(TCR-)]TJ /F11 1 Tf 12.1811 0 TD (ab)Tj /F6 1 Tf 1.1663 0 TD (/)Tj -13.3474 -1.3526 TD [(CD3/CD8)-307.4(downregulation)]TJ 0 -1.3607 TD (-)Tj 0 5.2498 -4.6661 0 185.3291 533.5936 Tm (51)Tj 0 6.9999 -6.9999 0 188.4472 538.6393 Tm [(Cr)-300.2(Release)-302.4(Assay)]TJ 15.8095 3.3935 TD [(-Human)-301.7(cytomegalovirus)]TJ 0 -1.3526 TD [(strains,)-300.6(UL40)-300.9(protein)]TJ 0 -1.3607 TD [(-Recombinant)-300.5(protein)-304.3(facility)]TJ T* [(of)-302.4(SFR26)-299.1(\(synthetic)-303(peptides\))]TJ 13.9305 2.041 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (35)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 -4.4059 TD [(SQAPLPCVL)-5687.3(9)]TJ 21.811 -1.1906 TD [(Murine)-302.3(TAP-2-de)]TJ /F7 1 Tf 7.2811 0 TD ()Tj /F6 1 Tf 0.5264 0 TD [(cient)-308.5(RMA-S)-296.5(cells)-4524.2(N/A)]TJ /F13 1 Tf 13.5175 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 0 TD (NK)Tj /F14 1 Tf 1.4092 0 TD ()Tj /F6 1 Tf 0.575 0 TD [(CTL-based)-297.5(T)-301.2(cell)-304.1(assay)]TJ 14.9024 0.6803 TD [(Epstein)-299(Barr)-309.2(Virus,)-298.4(Lytic)]TJ 0 -1.3607 TD [(switch)-300.9(protein)-304.3(BZLF1)]TJ 13.9305 0.6803 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (38)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 -1.1825 TD [(SQAQLPCLV)-5510.3(9)]TJ 0 -6.2768 TD [(VMAPRTLFL)-5479.3(9)]TJ 21.811 3.2234 TD [(Murine)-302.3(TAP-2-de)]TJ /F7 1 Tf 7.2811 0 TD ()Tj /F6 1 Tf 0.5264 0 TD [(cient)-308.5(RMA-S)-296.5(cells)-4524.2(N/A)]TJ /F13 1 Tf 13.5175 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 0 TD (NK)Tj /F14 1 Tf 1.4092 0 TD ()Tj /F6 1 Tf 0.575 0 TD [(CTL-based)-297.5(T)-301.2(cell)-304.1(assay)]TJ 14.9024 0.6803 TD [(Epstein)-299(Barr)-309.2(Virus,)-298.4(Lytic)]TJ 0 -1.3607 TD [(switch)-300.9(protein)-304.3(BZLF1)]TJ 13.9305 0.6803 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (38)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -75.3624 -4.4059 TD [(LCL)-298.7(721.221)-301.6(derivative)-304.5(cells)-304.5(transfected)-304.3(with)]TJ 0 -1.3607 TD [(HLA-Cw0401,)-299.8(-Cw1502,)-309.7(-G1,)-298.2(-Gs,)-299.8(and)-300(LCL)]TJ 19.5756 2.7213 TD [(Immunoprecipitation)-309.5(by)]TJ 0 -1.3607 TD [(anti-HLA)-299.1(class-I-pan)-303.3(W6/32)]TJ T* [(mAb)-301.2(crosslinked)-302.7(to)-299.5(protein)]TJ 0 -1.3526 TD [(A-Sepharose)-304.8(beads)]TJ 13.2259 2.7132 TD [(Finnigan)-305.4(TSQ)-301(7000)]TJ 0 -1.3607 TD [(LC-MS/MS)-306.8(system)]TJ 10.4398 0.6803 TD [(HLA-E)-305.2(peptide)-301.9(binding)-305.5(assay)]TJ 16.8867 0.6803 TD [(HLA-E)-297.1(and)-308.1(AEH)-299.3(proteins,)]TJ 0 -1.3607 TD [(expressed)-307(in)-300.5(721.221)-301.6(cells)]TJ 13.9305 0.6803 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (34)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 -4.4059 TD [(VTAPRTLLL)-5739.3(9)]TJ 21.811 -1.6927 TD [(LCL)-298.7(721.221)-301.6(derivative)-304.5(cells)-304.5(transfected)-304.3(with)]TJ 0 -1.3607 TD [(HLA-Cw0401,)-299.8(-Cw1502,)-309.7(-G1,)-298.2(-Gs,)-299.8(and)-300(LCL)]TJ 19.5756 2.7213 TD [(Immunoprecipitation)-309.5(by)]TJ 0 -1.3607 TD [(anti-HLA)-299.1(class-I-pan)-303.3(W6/32)]TJ T* [(mAb)-301.2(crosslinked)-302.7(to)-299.5(protein)]TJ 0 -1.3526 TD [(A-Sepharose)-304.8(beads)]TJ 13.2259 2.7132 TD [(Finnigan)-305.4(TSQ)-301(7000)]TJ 0 -1.3607 TD [(LC-MS/MS)-306.8(system)]TJ 10.4398 0.6803 TD [(HLA-E)-305.2(peptide)-301.9(binding)-305.5(assay)]TJ 16.8867 0.6803 TD [(HLA-E)-297.1(and)-308.1(AEH)-299.3(proteins,)]TJ 0 -1.3607 TD [(expressed)-307(in)-300.5(721.221)-301.6(cells)]TJ 13.9305 0.6803 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (34)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 0 TD [(VTAPRTVLL)-5573.3(9)]TJ 0 -2.373 TD [(VMAPRTVLL)-5302.3(9)]TJ 0 -2.3649 TD (YLLPRRGPRL)Tj 0 -1.3607 TD (\(LLPRRGPRL*\))Tj 11.1201 0.6803 TD [(10)-9732.9(Patients)-306.8(with)-295.3(chronic)-308.9(hepatitis)-301.3(C)-6333.6(N/A)]TJ /F13 1 Tf 32.0159 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 0 TD (IFN-)Tj /F11 1 Tf 1.9519 0 TD (g)Tj /F6 1 Tf 0.7046 0 TD [(ELISPOT)-305.5(CD8)]TJ 0 5.2498 -4.6661 0 365.6125 591.5338 Tm (+)Tj 0 6.9999 -6.9999 0 368.7306 596.6361 Tm [(T)-301.2(cell)-304.1(assay)]TJ 7.5241 0.6803 TD [(Hepatitis)-307.2(C)-299.7(Virus,)-298.4(Peptide)]TJ 0 -1.3607 TD [(HCV)-300.6(core)]TJ 13.9305 0.6803 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (39)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 -4.4059 TD [(PEIVIYDYM)-5769.2(9)]TJ 21.811 1.3526 TD [(-Cryopreserved)-309.5(human)-301.5(PBMCs)]TJ 0 -1.3526 TD [(-B-lymphoblastoid)-304.7(cell)-304.1(line)-298.6(721.221)-301.6(and)]TJ 0 -1.3606 TD [(murine)-302.4(RMA-S)-304.6(cells)]TJ 19.5756 1.3606 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 0.6722 TD [(Intracellular)-303.6(cytokine)-311.3(staining)]TJ 0 -1.3526 TD [(\(ICS\))-303(assay)]TJ 16.8867 1.3526 TD (Human)Tj 0 -1.3526 TD (Immunode)Tj /F7 1 Tf 4.4545 0 TD ()Tj /F6 1 Tf 0.5345 0 TD [(ciency)-303.5(Virus)]TJ 8.9414 0.6803 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (40)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 -4.4059 TD [(AISPRTLNA)-5885.2(9)]TJ 21.811 0.6722 TD [(-PBMCs)-305.5(derived)-301.9(from)-300.8(HIV-infected)-306.8(patients)]TJ 0 -1.3526 TD [(-HLA-E)-299.5(transfected)-312.4(K-562)-296.8(cells)]TJ 19.5756 0.6803 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 0 TD (NK)Tj /F14 1 Tf 1.4092 0 TD ()Tj /F6 1 Tf 0.575 0 TD [(CTL-based)-297.5(T)-301.2(cell)-304.1(assay)]TJ 14.9024 0.6722 TD [(Human)-299.3(Immunode)]TJ /F7 1 Tf 7.8319 0 TD ()Tj /F6 1 Tf 0.5264 0 TD (ciency)Tj -8.3583 -1.3526 TD [(Virus)-305.8(Gag)-300.3(polyprotein)]TJ 13.9305 0.6803 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (41)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 -3.7337 TD [(VMAPRALLL)-5395.3(9)-10211.9(PBMCs)-303.1(derived)-301.9(from)-300.8(donors)-7910(N/A)]TJ /F13 1 Tf 43.136 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 0.6803 TD (TAP-2)Tj /F14 1 Tf 0 5.2498 -4.6661 0 448.6676 549.9778 Tm ()Tj /F6 1 Tf 0.5723 0 TD (/)Tj /F14 1 Tf 0.324 0 TD ()Tj /F6 1 Tf 0 6.9999 -6.9999 0 451.7857 559.7858 Tm [(murine)-310.5(T)-293.1(cell)-304.1(lymphoma)]TJ -4.0982 -1.3606 TD [(RMA-S)-304.6(cell)-304.1(line)]TJ 16.8867 1.3606 TD [(Human)-299.3(Leukocyte)-309.4(Antigen-)]TJ 0 -1.3606 TD [(Cw7)-301.9(Leader)-304.6(Sequence)]TJ 13.9305 0.6803 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (42)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 -3.0453 TD [(PELAKSAPAPK)-4480.3(11)]TJ 21.811 -0.6803 TD (HLA)Tj /F14 1 Tf 0 5.2498 -4.6661 0 479.5085 242.2488 Tm ()Tj /F6 1 Tf 0 6.9999 -6.9999 0 482.6266 245.2535 Tm (/TPN)Tj 0 5.2498 -4.6661 0 479.5085 260.9574 Tm (+/-)Tj 0 6.9999 -6.9999 0 482.6266 269.6315 Tm [(LCL)-298.7(721.221)-301.6(and)-300(HLA)]TJ /F14 1 Tf 0 5.2498 -4.6661 0 479.5085 333.9779 Tm ()Tj /F6 1 Tf 0 6.9999 -6.9999 0 482.6266 336.9826 Tm (/)Tj -15.5098 -1.3606 TD (TPN)Tj 0 5.2498 -4.6661 0 489.033 241.8519 Tm (+/-)Tj 0 6.9999 -6.9999 0 492.1511 250.4693 Tm [(T2)-308.2(cell)-296(lines)-307.2(transduced)-305.7(with)]TJ -3.1506 -1.3526 TD (sHLA-E*01:03)Tj 19.5756 3.3935 TD [(Large-scale)-303.2(soluble)-302(HLA)]TJ 0 -1.3606 TD [(technology)-301.3(to)-299.5(isolate)-305.8(HLA-E)]TJ T* [(bound)-302.3(peptides,)-303.1(developed)]TJ 0 -1.3526 TD [(by)-297.4(\()]TJ 0.85098 0.19216 0.2 scn 1.5955 0 TD (43)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj 10.6666 4.0739 TD [(nano-LC)-303.4(Ultra)-299.2(2D)]TJ 0 -1.3606 TD [(HPLC)-301.5(system)-305(coupled)]TJ T* [(to)-299.5(Orbitrap)]TJ 0 -1.3526 TD [(mass)-307(spectrometer)]TJ 10.4398 2.0329 TD (NK)Tj /F14 1 Tf 1.4092 0 TD ()Tj /F6 1 Tf 0.575 0 TD [(CTL-based)-297.5(T)-301.2(cell)-304.1(assay)]TJ 14.9024 1.3606 TD [(Human,)-300(60)-305.5(kDa)-296.9(heat)-306.4(shock)]TJ 0 -1.3606 TD (protein,)Tj 0 -1.3526 TD [(mitochondrial)-300.5(precursor)]TJ 13.9305 1.3526 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (23)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 -1.1825 TD [(VGGTSDVEVNEK)-3273.3(12)]TJ /F15 1 Tf 93.6989 -3.1506 TD (\(Continued\))Tj /F1 1 Tf 6.9999 0 0 6.9999 49.6063 800.9573 Tm 0.49804 0.49804 0.49412 scn [(Weitzen)-302.7(et)-300.8(al.)]TJ 57.0665 0 TD (10.3389/fimmu.2024.1442783)Tj -57.0665 -109.3302 TD [(Frontiers)-304.6(in)]TJ 0.85098 0.19216 0.2 scn 5.5074 0 TD (Immunology)Tj 58.8564 0 TD (frontiersin.org)Tj 0.15294 0.15294 0.15294 scn -29.5213 0 TD (06)Tj ET 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531.779 70.923 10.261 4.989 re f 531.779 60.888 10.261 15.024 re f 531.779 60.888 0.737 714.387 re f BT /F5 1 Tf 0 6.9999 -6.9999 0 54.7653 70.6961 Tm 0.49804 0.49804 0.49412 scn 0 Tc 0 Tw [(TABLE)-298(1)-802.1(Continued)]TJ /F1 1 Tf 0 9 -9 0 76.7055 75.7418 Tm 1 1 1 scn (Peptide)Tj 0 -1.1087 TD (Sequence)Tj 8.6488 0.5543 TD [(Length)-5119.9(Material)]TJ 23.5402 0.5543 TD (Immunopeptidome)Tj 0 -1.1087 TD [(Enrichment)-300.8(Method)]TJ 10.2866 0.5543 TD [(MS)-302.1(Method)]TJ 8.1197 0.5543 TD [(HLA-E)-302.8(peptide)-303.5(recogni-)]TJ 0 -1.1087 TD [(tion)-303.9(by)-299.1(T-cell)-302.9(assay)]TJ 13.1339 0.5543 TD [(Origin)-7993.6(Ref)]TJ /F6 1 Tf 0 6.9999 -6.9999 0 112.4787 75.7418 Tm 0.15294 0.15294 0.15294 scn [(QMRPVSRVL)-5313.3(9)]TJ 21.811 1.3526 TD [(-K562)-304.9(and)-300(721.221)-301.6(cell)-304.1(lines)]TJ 0 -1.3526 TD [(-Two)-301(human)-301.5(CD94/NKG2A+)-303.7(\(but)-303.1(killer)-303.6(Ig-)]TJ 0 -1.3607 TD [(like)-302(receptor)-306.2([KIR])]TJ /F14 1 Tf 7.6213 0 TD ()Tj /F6 1 Tf 0.575 0 TD [(\))-296.8(cytotoxic)-306.2(NK)-303.9(cell)-296(lines)]TJ 11.3793 1.3607 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 0 TD (NK)Tj /F14 1 Tf 1.4092 0 TD ()Tj /F6 1 Tf 0.575 0 TD [(CTL-based)-297.5(T)-301.2(cell)-304.1(assay)]TJ 14.9024 1.3526 TD [(Human,)-300(60)-305.5(kDa)-296.9(heat)-306.4(shock)]TJ 0 -1.3526 TD (protein,)Tj 0 -1.3607 TD [(mitochondrial)-300.5(precursor)]TJ 13.9305 1.3607 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (44)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 -6.4469 TD [(SQQPYLQLQ)-5447.3(9)]TJ 21.811 2.7132 TD [(-Immature)-307.1(dendritic)-304.9(cells)-296.4(\(iDCs\))-309.9(treated)-296.4(by)]TJ 0 -1.3526 TD [(a)-300(peptic-tryptic)-308.1(digest)-304.2(of)-302.4(gliadin)-303.1(and)-300(single-)]TJ 0 -1.3607 TD (puri)Tj /F7 1 Tf 1.6684 0 TD ()Tj /F6 1 Tf 0.5264 0 TD (ed)Tj /F11 1 Tf 1.2392 0 TD (a)Tj /F6 1 Tf 0.6236 0 TD (-,)Tj /F11 1 Tf 0.8747 0 TD (b)Tj /F6 1 Tf 0.5426 0 TD (-,)Tj /F11 1 Tf 0.8747 0 TD (g)Tj /F6 1 Tf 0.405 0 TD [(-,)-302.7(or)]TJ /F11 1 Tf 2.041 0 TD (w)Tj /F6 1 Tf 0.6722 0 TD [(-gliadin)-305.5(fractions)]TJ -9.4679 -1.3526 TD [(-PBMCs)-305.5(and)-300(NK)-303.9(cells)-296.4(isolated)-304.1(from)-300.8(celiac)]TJ 0 -1.3607 TD [(patients)-308.3(or)-294.2(healthy)-306.6(donors)]TJ 19.5756 2.7132 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 1.3607 TD (-IFN-)Tj /F11 1 Tf 2.3002 0 TD (g)Tj /F6 1 Tf 0.413 0 TD [(-and)-302.4(TNF-)]TJ /F11 1 Tf 4.3816 0 TD (a)Tj /F6 1 Tf 0.9233 0 TD [(T)-301.2(cell)-304.1(ELISPOT)]TJ -8.0181 -1.3607 TD (assay)Tj 0 -1.3526 TD [(-Gliadin-DC-NK)-307.5(assay)]TJ 16.8867 2.0329 TD [(Human,)-300(Alpha/beta-gliadin)-309.6(A-)]TJ 0 -1.3526 TD [(I)-298.7(precursor)]TJ 13.9305 0.6722 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (45)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 -5.0863 TD [(ALALVRMLI)-5624.3(9)]TJ 21.811 -3.5555 TD [(Human)-307.4(B)-299.8(cell)-296(line;)-307.4(721.221)-301.6(cells)]TJ 19.5756 3.3935 TD [(Immunoprecipitation)-309.5(by)]TJ 0 -1.3526 TD [(anti-HLA)-299.1(class-I-pan)-303.3(W6/32)]TJ 0 -1.3607 TD [(mAb)-301.2(crosslinked)-302.7(to)-299.5(mouse)]TJ T* [(IgG-Sepharose)-302.7(beads)-306.2(to)]TJ 0 -1.3526 TD [(isolate)-297.7(HLA-E)-305.2(peptides)]TJ 0 -1.3607 TD [(from)-300.8(lysate)]TJ 13.2259 5.4345 TD [(RP-LC)-304(\(C18\))-299.9(coupled)]TJ 0 -1.3607 TD [(to)-299.5(Kratos)-307.1(Axima-CFR)]TJ T* (MALDI)Tj 0 -1.3526 TD [(mass)-307(spectrometer)]TJ 10.4398 2.0329 TD (NK)Tj /F14 1 Tf 1.4092 0 TD ()Tj /F6 1 Tf 0.575 0 TD [(CTL-based)-297.5(T)-301.2(cell)-304.1(assay)]TJ 14.9024 1.3607 TD [(Human,)-300(ATP-binding)-307.7(cassette)]TJ 0 -1.3607 TD [(transporter)-304.6(multidrug)]TJ 0 -1.3526 TD [(resistance-associated)-311(protein)]TJ 13.9305 1.3526 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (33)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 1.1906 TD [(GMKFDRGYI)-5332.3(9)]TJ 0 -2.373 TD [(AMAPRTLLL)-5478.3(9)]TJ T* [(RRYQKSTEL)-5687.3(9)]TJ T* [(AAVLEYL)-6800.2(7)]TJ 21.811 -5.7585 TD [(Synthesized)-305.3(peptides)-302.4(were)-305.7(loaded)-305.1(onto)]TJ 0 -1.3526 TD [(recombinant)-305.2(B-LCL)-301.3(721.221)-301.6(and)-300(TAP)]TJ 0 -1.3607 TD (de)Tj /F7 1 Tf 0.9395 0 TD ()Tj /F6 1 Tf 0.5264 0 TD [(cient)-308.5(T2)-300.1(expressing)-307.9(HLA-E)]TJ /F16 1 Tf 11.0067 0 TD ()Tj /F6 1 Tf 0.5183 0 TD (01:01)Tj 6.5846 4.0739 TD [(Large-scale)-303.2(af)]TJ /F7 1 Tf 5.475 0 TD ()Tj /F6 1 Tf 0.5345 0 TD (nity)Tj -6.0096 -1.3607 TD (puri)Tj /F7 1 Tf 1.6603 0 TD ()Tj /F6 1 Tf 0.5345 0 TD [(cation)-305.9(of)-294.3(sHLA-E)]TJ -2.1949 -1.3526 TD [(molecules)-300.1(to)-299.5(isolate)-305.8(and)]TJ 0 -1.3607 TD [(identify)-305.1(sHLA-E-bound)]TJ 0 -1.3526 TD [(peptides)-302.4(\()]TJ 0.85098 0.19216 0.2 scn 3.9605 0 TD (46)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj 8.3016 4.7461 TD [(nano-LC)-303.4(Ultra)-299.2(2D)]TJ 0 -1.3526 TD [(HPLC)-301.5(coupled)-308.3(to)-299.5(an)]TJ 0 -1.3606 TD [(Orbitrap)-304.5(ion)-302.7(trap)]TJ T* [(mass)-307(spectrometer)]TJ 10.4398 2.041 TD (NK)Tj /F14 1 Tf 1.4092 0 TD ()Tj /F6 1 Tf 0.575 0 TD [(CTL-based)-297.5(T)-301.2(cell)-304.1(assay)]TJ 14.9024 0.6803 TD [(Human,)-300(Histone)-307.3(H2A)-301.3(type)]TJ 0 -1.3606 TD (2-B)Tj 13.9305 1.3606 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (47)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj 0 6.9999 -6.9999 0 314.1353 746.8156 Tm (**)Tj -95.8694 5.4183 TD [(VMAPRTLFL)-5479.3(9)]TJ 0 -2.3649 TD [(SKGKIYPVGYY)-4437.3(11)]TJ 0 -2.373 TD [(DVHDGKVVSTHEQ)-2294.3(13)]TJ T* [(PKKTESHHKAKGK)-2669.3(13)]TJ T* [(LGHPDTLNQGEFKEL)-1658.4(15)]TJ T* [(LVDSGAQVSVVHPNL)-1410.4(15)]TJ T* [(SLQGRTLIL)-6051.2(9)]TJ 21.811 -9.9862 TD [(B6-CIITA-Ed)-307.9(cells)-296.4(infected)-304.5(either)-302.8(as)-300.5(mock)]TJ 0 -1.3607 TD [(or)-302.3(PR8)]TJ 19.5756 4.0739 TD [(Immunoprecipitation)-309.5(by)]TJ 0 -1.3607 TD [(anti-MHC-II)-300.7(antibody)-304.9(\(clone)]TJ 0 -1.3526 TD [(M5/114.5\))-299.7(cross-linked)-305.1(to)]TJ 0 -1.3607 TD [(Protein)-302.8(G)-296.3(Sepharose)-309(beads)]TJ T* [(to)-299.5(isolate)-297.7(peptide-MHC-II)]TJ 0 -1.3526 TD [(complexes,)-301.4(described)-306.1(in)-300.5(\()]TJ 0.85098 0.19216 0.2 scn 10.213 0 TD (48)Tj 0.15294 0.15294 0.15294 scn 0.9557 0 TD (\))Tj 2.0572 7.5322 TD [(UltiMate)-306.7(3000)]TJ 0 -1.3526 TD [(RSLCnano)-304.3(System)]TJ 0 -1.3606 TD [(\(PepMap)-310(C18)]TJ 0 -1.3526 TD [(column\))-303.2(coupled)-308.3(to)]TJ 0 -1.3607 TD [(an)-301.7(Orbitrap)-304.5(Fusion)]TJ 0 -1.4659 TD [(Lumos)-302.1(Tribrid)]TJ /F17 1 Tf 0 7.9999 -7.9999 0 468.7369 499.4645 Tm ()Tj /F6 1 Tf 0 6.9999 -6.9999 0 479.5085 458.022 Tm [(mass)-307(spectrometer)]TJ 10.4398 4.8271 TD (IFN-)Tj /F11 1 Tf 1.9519 0 TD (g)Tj /F6 1 Tf 0.7046 0 TD [(enzyme-linked)-307(immunosorbent)]TJ -2.6565 -1.3607 TD [(spot)-306.4(\(ELISpot\))-301.6(assay)]TJ 16.8867 0.6803 TD (In)Tj /F7 1 Tf 0.8747 0 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[(with)-303.4(the)-298(rhesus)-308.2(cytomegalovirus)-304.7(68-1)-298.6(strain)]TJ 0 -1.3526 TD [([RhCMV68-1-HIVGag)-309.4(vector)-297.8(SIV)-308.1(vaccine)]TJ 0 -1.3607 TD [(where)-301.2(rhesus)-308.2(macaques)-299.7(stimulate)-305.7(Mamu-E-)]TJ T* [(restricted)-309.5(responses)-297.9(to)-307.6(RL9HIV)-295.5(epitope].)]TJ 19.5756 4.7542 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 4.7461 TD [(Flow)-304.6(cytometric)-305.8(ICS)-300.5(assay)-296.9(used)-302(to)]TJ 0 -1.3526 TD [(measure)-307.6(CD8)]TJ 0 5.2498 -4.6661 0 109.3606 569.5936 Tm (+)Tj 0 6.9999 -6.9999 0 112.4787 574.6959 Tm [(T)-301.2(cell)-304(responses)]TJ -6.2282 -1.3607 TD [(\(intracellular)-309(expression)-306.4(of)-302.4(IFN)]TJ /F11 1 Tf 12.659 0 TD (g)Tj /F6 1 Tf 0.7127 0 TD (and)Tj -13.3717 -1.3526 TD (TNF)Tj /F11 1 Tf 1.879 0 TD (a)Tj /F6 1 Tf 0.6236 0 TD [(\))-296.8(i)0(n)-300.5(mononuclear)-307.7(cell)]TJ -2.5026 -1.3607 TD [(preparations)-304.4(from)-308.9(blood)]TJ 0 -1.3526 TD [(-HLA-E)-307.6(peptide)-301.9(binding)-305.5(af)]TJ /F7 1 Tf 10.9257 0 TD ()Tj /F6 1 Tf 0.5265 0 TD [(nity)-301.8(assay)]TJ -11.4522 -1.3607 TD [(by)-305.5(micro-scale)-303(refold-sandwich)]TJ T* [(ELISA)-302.4(method)-304.5(\()]TJ 0.85098 0.19216 0.2 scn 6.6089 0 TD (50)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (,)Tj 0.85098 0.19216 0.2 scn 0.5183 0 TD (51)Tj 0.15294 0.15294 0.15294 scn 0.9557 0 TD (\))Tj 7.84 6.1148 TD [(Mycobacterium)-306.9(tuberculosis)]TJ 0 -1.3607 TD [(44,)-298.1(Enoyl-[acyl-carrier-)]TJ T* [(protein])-301.5(reductase)-310.9([NADH])]TJ 13.9305 1.3607 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (52)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 -7.1191 TD (SMADRAENL)Tj 11.1201 -10.6747 TD (9)Tj 10.6909 2.7132 TD [(-PBMCs)-305.5(derived)-301.9(from)-300.8(healthy)-306.6(adults)-296(who)]TJ 0 -1.3526 TD [(produced)-306.1(or)-302.3(lacked)-298.7(IFN)]TJ /F11 1 Tf 9.6704 0 TD (g)Tj /F6 1 Tf 0.7046 0 TD [(in)-300.5(response)-305.5(to)]TJ -10.375 -1.3607 TD [(Mtb-derived)-302(PPD)]TJ T* [(-PBMCs)-305.5(from)-300.8(infants)-301.3(vaccinated)-308.3(with)-295.3(M.)]TJ 0 -1.3526 TD [(bovis)-299.2(Bacillus)-305.5(Calmette)-304.8(Guerin)-301.1(\(BCG\))]TJ 19.5756 2.7132 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 4.7461 TD (-IFN)Tj /F11 1 Tf 1.9519 0 TD (g)Tj /F6 1 Tf 0.7046 0 TD [(ELISA)-302.3(T)-301.2(cell)-304.1(assay)]TJ -2.6565 -1.3526 TD [(-Single)-306.4(HLA-E)-297.1(expressing)-307.9(cell)-304.1(lines)]TJ 0 -1.3607 TD [(\(K562)-307.8(cells\))-301.7(were)-297.6(used)-302(to)-299.5(assess)-307.1(T)-301.2(cell)]TJ 0 -1.3526 TD [(responses)-306(to)-299.5(T)-301.2(cell)-304.1(lines,)-299.8(HLA-E)]TJ 0 -1.3607 TD [(transfectants)-309.1(loaded)-305.2(with)-295.3(peptides)]TJ 0 -1.3526 TD [(\(labeled)-302.1(with)-303.4(1)-298.5(Ci)]TJ 0 5.2498 -4.6661 0 271.8991 587.2251 Tm (51)Tj 0 6.9999 -6.9999 0 275.0172 592.2141 Tm [(Cr\),)-306.1(and)]TJ -8.7309 -1.3607 TD [(cytotoxic)-306.2(CD8)]TJ 0 5.2498 -4.6661 0 281.4236 571.6345 Tm (+)Tj 0 6.9999 -6.9999 0 284.5417 576.7369 Tm [(T)-301.2(cell)-304.1(clones)]TJ -6.5198 -1.3607 TD [(from)-300.8(PBMCs)]TJ 16.8867 15.4288 TD [(Mtb)-297.3(HRv1286)]TJ 13.9305 -9.9943 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (53)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -1.304 -1.3607 TD (***)Tj -95.8694 8.9819 TD (WMCDRAVDL)Tj 81.9389 0 TD [(Mtb)-297.3(HRv2954c)]TJ -81.9389 -2.373 TD (SMAGRAGQL)Tj 81.9389 0 TD [(Mtb)-297.3(HRv3282)]TJ -81.9389 -2.373 TD (EMVLRADQL)Tj 81.9389 0 TD [(Mtb)-297.3(HRv0191)]TJ -81.9389 -2.3731 TD (DMLGRAGGL)Tj 81.9389 0 TD [(Mtb)-297.3(HRv3015)]TJ -81.9389 -2.3649 TD (EMKTDAATL)Tj 81.9389 0 TD [(Mtb)-297.3(HRv3874)]TJ -81.9389 -2.373 TD (EMGRAPLDL)Tj 81.9389 0 TD [(Mtb)-297.3(HRv2627c)]TJ -81.9389 -2.373 TD (EMLTSRGLL)Tj 81.9389 0 TD [(Mtb)-297.3(HRv1997)]TJ -81.9389 -2.373 TD (GMGMVGTAL)Tj 81.9389 0 TD [(Mtb)-297.3(HRv1737c)]TJ -81.9389 -2.373 TD (PMADIAAAL)Tj 81.9389 0 TD [(Mtb)-297.3(HRv1253)]TJ -81.9389 -2.3649 TD (GMQFDRGYL)Tj 11.1201 -7.1191 TD (9)Tj 10.6909 4.7542 TD [(-EBV-transformed)-306.3(lymphoblastoid)-307.9(B)-299.8(cell)]TJ 0 -1.3607 TD [(lines)-299.1(\(B-LCL\),)-304.4(blasts,)-302.6(and)-308.1(macrophages)-301.1(from)]TJ T* [(PBMC)-302.6(derived)-301.9(from)-300.8(donors)-304.9(who)-300.6(took)]TJ 0 -1.3526 TD [(Ty21a)-303(vaccine)]TJ 0 -1.3607 TD [(-The)-304.2(HLA)-297.9(class)-307.2(I-de)]TJ /F7 1 Tf 8.253 0 TD ()Tj /F6 1 Tf 0.5345 0 TD [(cient)-300.4(B)-299.8(cell)-304.1(line)]TJ -8.7876 -1.3526 TD [(721.221)-301.6(and)-300(its)-305.1(transfectants)-309.1(\(721.221.AEH/)]TJ 0 -1.3607 TD [(E6\))-300.7(transfected)-304.3(with)-303.4(HLA-E,)-305.9(expressing)]TJ 0 -1.3526 TD (HLA-E*01:01)Tj 19.5756 4.7461 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 11.4765 0 TD (N/A)Tj /F13 1 Tf 1.7494 0 TD ()Tj /F6 1 Tf 8.6904 0.6803 TD [(The)-301.8(human)-301.5(granzyme)-309.7(B)-299.8(IFN-)]TJ /F11 1 Tf 12.0434 0 TD (g)Tj /F6 1 Tf -12.0434 -1.3607 TD [(ELISPOT)-305.5(assay)]TJ 16.8867 1.3607 TD [(Salmonella)-304.2(enterica)-306.4(serovar)]TJ 0 -1.3607 TD [(typhi,)-302.8(Chaperonin)-304.2(GroEL)]TJ 13.9305 0.6803 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (54)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 4.7461 TD (AMLQDIATL)Tj 0 -2.3731 TD (KMLRGVNVL)Tj T* (VEGEALATL)Tj T* (AAVEELKAL)Tj T* (AVAKAGKPL)Tj 0 -2.365 TD (KLQERVAKL)Tj 0 -5.0863 TD [(552)-296.2(HLA-E)-305.2(peptides)]TJ 0 -1.3607 TD [(\(424)-301.5(9-mer\))]TJ 11.1201 0.6803 TD (8-13)Tj 10.6909 3.3935 TD [(-HLA-I)-301.7(negative)-308.1(K562)-294.4(cells)-304.5(transfected)-304.3(with)]TJ 0 -1.3607 TD (HLA-E*01:03)Tj 0 -1.3526 TD [(-K562.HLA-E.B8)-302.7(and)-308.1(K562.HLA-E.UL49.5)]TJ 0 -1.3607 TD (cells)Tj 0 -1.3526 TD (-Qa-1)Tj 0 5.2498 -4.6661 0 503.2062 244.9701 Tm (b)Tj 0 6.9999 -6.9999 0 506.2676 249.6756 Tm [(mouse)-303.7(homolog)-304.3(of)-302.4(HLA-E)]TJ -3.0372 -1.3607 TD [(\(expressing)-305.1(H2-D)]TJ 0 5.2498 -4.6661 0 512.6739 278.4189 Tm (b)Tj 0 6.9999 -6.9999 0 515.792 281.0267 Tm (/K)Tj 0 5.2498 -4.6661 0 512.6739 288 Tm (b)Tj 0 6.9999 -6.9999 0 515.792 290.6078 Tm (\))Tj 10.6909 6.7871 TD (Af)Tj /F7 1 Tf 0.98 0 TD ()Tj /F6 1 Tf 0.5264 0 TD [(nity)-301.8(chromatography)-303(by)]TJ -1.5064 -1.3607 TD [(antibody)-296.8(W6/32)-302.1(cross-linked)]TJ 0 -1.3526 TD [(to)-299.5(the)-298(Sepharose)-309(beads)]TJ 0 -1.3607 TD [(followed)-297.4(by)-305.5(10)-297.4(kD)]TJ /F7 1 Tf 7.7428 0 TD ()Tj /F6 1 Tf 0.5264 0 TD (ltration)Tj -8.2692 -1.3526 TD [(and)-300(RP-HPLC)]TJ 0 -1.3607 TD [(C18)-297.4(fractionation)]TJ 13.2259 6.7871 TD [(Thermo)-302.4(LTQ-FT)]TJ 0 -1.3607 TD [(Ultra)-307.3(mass)]TJ 0 -1.3526 TD [(spectrometer)-307.4(operated)]TJ 0 -1.3607 TD [(in)-300.5(data-dependent)]TJ 0 -1.3526 TD (acquisition)Tj 0 -1.3607 TD [(\(DDA\))-303.4(mode)]TJ 10.4398 3.3935 TD 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-1.3858 TD [(note,)-341.1(this)-338.3(group)-346.6(employed)-341(an)-344.8(anti-MHC-II)-340.9(antibody)-343.4(that)-342.9(actually)]TJ 0 -1.3921 TD [(cross-reacted)-266.2(against)-268.7(Qa-1,)-267.5(leading)-267.6(to)-268.9(the)-262.8(unexpected)-271.3(isolation)-271(and)]TJ 0 -1.3858 TD [(detection)-207.3(of)-210.6(the)-206.1(cryptic)-212(epitope.)-205.5(With)-213(this)-206(notable)-209.9(cross-reactivity)-213.8(in)]TJ 0 -1.3921 TD [(mind,)-300.9(it)-294.7(has)-299.5(been)-300.2(found)-305.1(that)-298.9(antibodies)-303.1(3D12)-297(and)-303.6(4D12)-297(recognize)]TJ 0 -1.3858 TD [(HLA-E)-495(without)-504.8(cross-reacting)-503.8(with)-504(classical)-497.3(HLA-Ia)-507.4(molecules,)]TJ 0 -1.3921 TD 0.0237 Tc [(serving)-519.9(a)-5.4(s)-519.4(p)-4.3(otential)-520(antibodi)-6.1(e)5.1(s)-525.7(f)-0.3(or)-525.7(future)-524.1(HLA-E-speciali)-6.1(z)5.1(ed)]TJ 0 -1.3858 TD 0.0414 Tc [(i)5.3(m)-3.3(munopeptidomic)-544.2(procedures)-539.5(\()]TJ 0.85098 0.19216 0.2 scn 14.9102 0 TD 0.0375 Tc (62)Tj 0.15294 0.15294 0.15294 scn 1.0394 0 TD 0.0418 Tc [(\).)-540.2(However,)-540.2(it)-538.1(is)-539.1(worth)]TJ -15.9496 -1.3921 TD 0 Tc [(mentioning)-458.7(that)-462.6(these)-459.2(two)-461.1(antibodies)-460.6(have)-463.5(dif)63.1(ferent)-466.8(recognition)]TJ 0 -1.3858 TD [(patterns)-300.4(that)-298.9(could)-307.4(impact)-301.8(immunopeptidome)-307.8(recovery.)]TJ /F1 1 Tf 13.5 0 0 13.5 304.8944 564.8881 Tm 0.85098 0.19216 0.2 scn [(Utilizing)-300.7(MHC-E-restricted)-307.2(CD8)]TJ 10.1248 0 0 8.9989 502.4125 570.8975 Tm (+)Tj 13.5 0 0 13.5 513.2975 564.8881 Tm (T)Tj -15.4373 -1.1465 TD [(cells)-300.1(in)-301.9(vaccines)-304.3(and)-301.4(immunotherapies)]TJ 0 -1.1507 TD [(against)-301(pathogens;)-306.7(viruses)-303.2(and)-301.4(cancer)]TJ /F6 1 Tf 9 0 0 9 318.8409 508.8755 Tm 0.15294 0.15294 0.15294 scn [(MHC-E-restricted)-408.9(CD8)]TJ 6.7499 0 0 5.9992 404.9574 512.9007 Tm (+)Tj 9 0 0 9 412.4408 508.8755 Tm [(T)-406.5(cells)-401.6(in)-402.1(T-cell-based)-406.6(vaccines)-408.5(and)]TJ -11.9496 -1.3858 TD 0.0347 Tc [(i)4.9(m)-3.7(mune)-538.3(therapies)-533.6(o)-0.7(f)61.1(f)-1.9(er)-533.6(distin)]TJ 13.537 0 TD 0.0348 Tc [(ct)-538.8(advantages)-533.5(over)-533.5(classically)]TJ -13.537 -1.3921 TD 0.0281 Tc [(res)-4.7(t)-3.8(ri)-8(ct)-3.8(ed)-535.4(CD8)]TJ 6.7499 0 0 5.9992 363.5149 487.8991 Tm 0 Tc (+)Tj 9 0 0 9 372.4723 483.8739 Tm 0.0294 Tc [(T)-528.3(cel)-4.1(ls)-3.5(.)-533.7(H)2(LA-E)-536.2(mo)-6(lecu)-4.9(les)-532.6(a)0.3(r)-3.5(e)-531(u)-4.9(b)0.3(iquitou)-4.9(s)-3.5(ly)]TJ -7.5087 -1.3858 TD 0 Tc [(expressed)-492.1(and)-498.8(have)-488.7(only)-493.5(two)-492.6(major)-490.5(allomorphs,)-501.2(which)-488.9(possess)]TJ 0 -1.3921 TD [(identical)-409(peptide-binding)-421.4(grooves.)-413.3(This)-410.5(characteristic)-422(enables)-414.6(the)]TJ 0 -1.3858 TD 0.0187 Tc [(desi)-4.8(gn)-517.5(o)-4.1(f)-515.5(va)-4.1(cci)-4.8(nes)-518.1(a)-4.1(nd)-519.6(the)-6.2(r)-1.6(ap)-3(ie)-6.2(s)-518.1(f)1(o)-4.1(r)-518.1(th)-4.6(e)-516.5(g)-2(ene)-6.2(r)-1.6(al)-518.7(p)-3(o)2.2(p)-3(u)-3(l)-2.2(ati)-4.8(o)2.2(n,)]TJ 0 -1.3921 TD 0 Tc [(eliminating)-426.7(the)-432.9(need)-431.4(for)-426.1(personalized)-438.5(approaches)-433.5(often)-425.6(required)]TJ 0 -1.3858 TD [(with)-264.6(MHC-Ia-restricted)-270.2(T)-267.9(cells)-263(\()]TJ 0.85098 0.19216 0.2 scn 12.9953 0 TD (49)Tj 0.15294 0.15294 0.15294 scn 0.9575 0 TD [(\).)-270.5(For)-266.4(instance,)-265.6(in)-269.9(the)-262.8(context)-267.6(of)]TJ -13.9528 -1.3921 TD 0.0309 Tc [(pathogen)-530.5(infections,)-532.2(a)-527.4(recent)-523.8(s)-2(tudy)-531.6(described)-532.6(a)-527.4(TCR-based)]TJ 0 -1.3858 TD 0 Tc (bispeci)Tj /F7 1 Tf 2.7339 0 TD ()Tj /F6 1 Tf 0.5354 0 TD [(c)-409.2(molecule)-407.4(that)-405.9(potently)-408.6(and)-410.7(selectively)-409.7(binds)-410.3(HLA-E)-413.1(in)]TJ -3.2693 -1.3921 TD [(complex)-246.3(with)-245.7(a)-249.6(peptide)-246(encoded)-255.2(by)-245.1(the)]TJ /F15 1 Tf 15.811 0 TD (inh)Tj /F6 1 Tf 1.3165 0 TD [(A)-245.3(gene)-247.7(of)-248.4(Mtb)-246.8(\()]TJ 0.85098 0.19216 0.2 scn 6.3244 0 TD (63)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD [(\).)-245.3(The)]TJ -24.4158 -1.3858 TD [(authors)-245(demonstrated)-257.1(the)-250.2(elimination)-252.5(of)-248.4(Mtb-infected)-252.1(cells)-250.5(and)-253.2(the)]TJ 0 -1.3921 TD [(reduction)-394(of)-399.5(intracellular)-402.4(Mtb)-391.7(growth.)-395.6(Moreover,)-401.8(the)-395.1(researchers)]TJ 0 -1.3858 TD [(revealed)-515.1(the)-508.5(biophy)-8.9(sica)-9.6(l)-499.6(and)-511.4(structura)-14.1(l)-505.9(bases)-508(unde)-12.4(rpinn)-10.2(ing)-504.8(the)]TJ T* [(potency)-506.8(and)-505.1(speci)]TJ /F7 1 Tf 7.6472 0 TD ()Tj /F6 1 Tf 0.5291 0 TD [(city)-504.2(of)-506.6(their)-508.1(TCR-based)-507.6(bispec)-12.2(i)]TJ /F7 1 Tf 13.348 0 TD ()Tj /F6 1 Tf 0.5354 0 TD [(c)-497.4(molecul)-10.8(e,)]TJ -22.0598 -1.3921 TD [(thereby)-490.1(providing)-490.6(a)-489(proof)-495.8(of)-487.7(principle)-490.8(for)-495.4(an)-489.7(innovative)-488.4(TCR-)]TJ 0 -1.3858 TD 0.0593 Tc [(b)5(a)-1.3(sed)-560.9(t)2.2(hera)5(peutic)-557.8(stra)5(tegy)-553.6(ca)5(pab)5(l)0.6(e)-557.8(o)-1.3(f)-556.8(s)1.3(urmounting)-553.6(HLA)]TJ 0 -1.3921 TD 0 Tc [(polymorphism)-439.7(hurdles)-449.2(and)-442.1(thus)-437.3(promising)-443.8(broader)-445.4(applicability)]TJ 0 -1.3858 TD [(across)-301.4(patient)-302.3(populations.)]TJ 1.5496 -1.3921 TD [(Within)-331.7(the)-325.8(realm)-328.9(of)-324(vaccines,)-329.1(MHC-E-restricted)-333.3(CD8)]TJ 6.7499 0 0 5.9992 515.3951 275.4141 Tm (+)Tj 9 0 0 9 522.1983 271.3889 Tm [(T)-324.6(cells)]TJ -24.1449 -1.3858 TD [(have)-318.6(demonstrated)-326.4(distinctive)-323(protective)-323.1(abilities)-321.1(against)-319.1(HIV/SIV)]TJ 0 -1.3921 TD 0.0556 Tc [(infections)-550.5(\()]TJ 0.85098 0.19216 0.2 scn 5.3921 0 TD 0.0564 Tc (64)Tj /F8 1 Tf 0.15294 0.15294 0.15294 scn 1.0709 0 TD 0 Tc ()Tj /F6 1 Tf 0.85098 0.19216 0.2 scn 0.5669 0 TD 0.0564 Tc (66)Tj 0.15294 0.15294 0.15294 scn 1.0646 0 TD [(\).)-550.8(HI)5.1(V)-556.8(i)1.4(nfe)6.3(c)0(tion)-555.4(is)-556(g)4.2(e)0(ner)4.7(a)2.1(lly)-556.5(k)3.7(nown)-555.4(to)]TJ -8.0945 -1.3858 TD 0.043 Tc [(downregulate)-536.3(classi)6.9(cal)-544.8(H)3(LA-I)4.3(a)-540.5(a)1.3(nti)6.9(g)-2.9(en)-537.3(expression)-537.3(via)-540.5(N)0.9(ef)]TJ 0 -1.3921 TD 0 Tc [(proteins)-474.7(\()]TJ 0.85098 0.19216 0.2 scn 4.0819 0 TD (67)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD [(\).)-472.1(In)-474(contrast,)-474.1(HLA-E)-476.1(expression)-479.9(is)-472.1(not)-477.5(af)66.6(fected)-479.9(by)]TJ -5.0457 -1.3858 TD [(HIV)-389.5(infection)-391.1(and)-391.8(is)-383.9(continuously)-396.5(expressed)-397.6(to)-388.5(shield)-386.5(cells)-389(from)]TJ 0 -1.3921 TD [(NK-mediated)-220.9(lysis)-225.2(\()]TJ 0.85098 0.19216 0.2 scn 7.9244 0 TD (68)Tj 0.15294 0.15294 0.15294 scn 0.9575 0 TD [(\).)-220.1(The)-221.7(continuous)-228.9(expression)-221.6(of)-223.2(HLA-E)-217.8(upon)]TJ -8.8819 -1.3858 TD [(infection)-265.1(has)-268(sparked)-266.3(interest)-266.5(in)-263.6(identifying)-272.5(and)-265.8(_targeting)-266.7(MHC-E-)]TJ 0 -1.3921 TD [(associated)-336.7(epitopes)-336.6(for)-337.9(vaccine)-337.9(strategies)-335.9(against)-338(other)-337.4(viruses.)-336.8(To)]TJ 0 -1.3858 TD [(this)-250.1(end,)-257.7(an)-256.6(important)-258(aspect)-259.9(of)-254.7(consideration)-260.8(is)-257.9(the)-256.5(consistent)-258.5(and)]TJ 0 -1.3921 TD [(stable)-382.9(presentation)-385(of)-386.9(viral)-385.3(peptides)-385.9(within)-383.3(the)-382.5(HLA-E)-387.9(groove)-384.3(to)]TJ 0 -1.3858 TD [(ensure)-471.9(robust)-467.3(on-_target)-477.1(T-cell)-471.6(activity)-470.8(and)-473.6(clinical)-470.8(ef)58.2(fectiveness.)]TJ 0 -1.3921 TD [(High)-309.1(stability)-306.3(is)-314.6(particularly)-310.2(challenging)-317.6(for)-306.4(non-classical)-318.8(pHLA-E)]TJ 0 -1.3858 TD [(complexes,)-287.7(as)-288.8(they)-289.8(have)-287.1(been)-293.9(shown)-284.6(to)-287.8(be)-293.4(less)-287.9(stable)-288.5(than)-286.6(classical)]TJ 0 -1.3921 TD [(pHL)-9.5(A-)-11(I)-7.2(a)-507.9(co)-10(m)-6.9(p)0(l)-11.1(e)0(x)-10.5(e)0(s)-511.3(\()]TJ 0.85098 0.19216 0.2 scn 9.222 0 TD (18)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD [(\))-9.8(.)-506.4(Fo)-13.1(r)-505.3(i)0(n)-11.6(s)-7.7(ta)-10.6(nce)-12.8(,)-512.7(Wal)-14.3(lac)-12(e)-510(e)0(t)-516.6(al.)-518.6(r)-7.7(ece)-11.8(n)-7(t)-6.7(ly)]TJ -10.1858 -1.3858 TD 0.0199 Tc [(em)6.7(plo)3.4(y)5.5(ed)-518.4(im)6.7(muno)3.4(pep)4.5(t)0.6(ido)3.4(m)0.4(ics)-516.9(i)2.7(n)-522.6(a)3.4(n)-516.3(e)1.3(f)65.2(f)2.2(ort)-515.9(t)6.9(o)-519.5(i)2.7(dentify)-517.4(s)-0.4(t)6.9(a)-2.9(b)3.4(l)-1(e)]TJ 0 -1.3921 TD 0.0104 Tc [(HLA-)10.4(E)-5.9(-)-1(bound)-509(HIV-d)-5(e)10.4(r)-9.6(i)-0.5(ved)-509(p)1.3(ep)-5(tides)-513.8(t)3.7(hat)-512.8(c)10.4(o)-5.9(u)-5(ld)-509(be)-512.2(utilized)-509(to)]TJ ET 0.49804 0.50196 0.50196 scn 64.857 70.923 27.609 573.335 re f 64.857 644.258 m 64.857 741.77 l 92.466 741.77 l 92.466 644.258 l f* 64.857 741.77 27.609 23.528 re f 0.63529 0.63529 0.63529 scn 49.606 765.297 180.567 0.22681 re f 49.606 70.639 180.567 0.28348 re f 229.946 70.923 0.22678 694.375 re f 64.857 765.297 m 64.857 70.923 l 64.857 765.297 l f* 92.239 70.923 0.22676 77.613 re f 64.857 148.535 27.609 0.28348 re f 92.466 223.37 m 92.466 148.819 l 92.239 148.819 l 92.239 223.37 l 92.466 223.37 l f* 64.857 223.37 27.609 0.22678 re f 92.239 223.597 0.22676 136.8 re f 64.857 360.397 27.609 0.22678 re f 92.239 360.624 0.22676 92.353 re f 64.857 452.976 27.609 0.28348 re f 92.239 453.26 0.22676 72.794 re f 64.857 526.053 27.609 0.28345 re f 92.239 526.337 0.22676 117.921 re f 64.857 644.258 27.609 0.22681 re f 92.239 644.485 0.22676 97.285 re f 64.857 741.77 27.609 0.22681 re f 92.239 741.997 0.22676 23.301 re f 165.827 70.923 0.28346 77.613 re f 166.11 148.535 m 92.466 148.535 l 92.466 148.819 l 166.11 148.819 l 166.11 148.535 l f* 166.11 223.37 m 166.11 148.819 l 165.827 148.819 l 165.827 223.37 l 166.11 223.37 l f* 166.11 223.37 m 92.466 223.37 l 92.466 223.597 l 166.11 223.597 l 166.11 223.37 l f* 165.827 223.597 0.28346 136.8 re f 166.11 360.397 m 92.466 360.397 l 92.466 360.624 l 166.11 360.624 l 166.11 360.397 l f* 165.827 360.624 0.28346 92.353 re f 166.11 452.976 m 92.466 452.976 l 92.466 453.26 l 166.11 453.26 l 166.11 452.976 l f* 165.827 453.26 0.28346 72.794 re f 166.11 526.053 m 92.466 526.053 l 92.466 526.337 l 166.11 526.337 l 166.11 526.053 l f* 165.827 526.337 0.28346 117.921 re f 166.11 644.258 m 92.466 644.258 l 92.466 644.485 l 166.11 644.485 l 166.11 644.258 l f* 165.827 644.485 0.28346 97.285 re f 166.11 741.77 m 92.466 741.77 l 92.466 741.997 l 166.11 741.997 l 166.11 741.77 l f* 165.827 741.997 0.28346 23.301 re f 182.438 70.923 0.22678 77.613 re f 229.946 148.535 m 166.11 148.535 l 166.11 148.819 l 229.946 148.819 l 229.946 148.535 l f* 229.946 223.37 m 166.11 223.37 l 166.11 223.597 l 229.946 223.597 l 229.946 223.37 l f* 229.946 360.397 m 166.11 360.397 l 166.11 360.624 l 229.946 360.624 l 229.946 360.397 l f* 229.946 452.976 m 166.11 452.976 l 166.11 453.26 l 229.946 453.26 l 229.946 452.976 l f* 229.946 526.053 m 166.11 526.053 l 166.11 526.337 l 229.946 526.337 l 229.946 526.053 l f* 229.946 644.258 m 166.11 644.258 l 166.11 644.485 l 229.946 644.485 l 229.946 644.258 l f* 229.946 741.77 m 166.11 741.77 l 166.11 741.997 l 229.946 741.997 l 229.946 741.77 l f* 199.049 70.923 0.22678 77.613 re f 64.63 70.923 0.45354 694.375 re f /Cs6 cs 1 1 1 scn 45.298 70.696 19.332 0.22675 re f 45.298 765.297 19.332 0.22681 re f /Cs6 cs 1 1 1 scn 45.298 70.696 19.332 4.989 re f 45.298 60.661 19.332 15.024 re f 45.298 760.535 19.332 4.989 re f 45.298 760.535 19.332 15.024 re f 54.765 60.661 9.8646 714.897 re f BT /F5 1 Tf 0 6.9999 -6.9999 0 54.7653 70.6961 Tm 0.49804 0.49804 0.49412 scn 0 Tc [(TABLE)-298(1)-802.1(Continued)]TJ /F1 1 Tf 0 9 -9 0 76.7055 75.7418 Tm 1 1 1 scn (Peptide)Tj 0 -1.1087 TD (Sequence)Tj 8.6488 0.5543 TD [(Length)-5119.9(Material)]TJ 23.5402 0.5543 TD (Immunopeptidome)Tj 0 -1.1087 TD [(Enrichment)-300.8(Method)]TJ 10.2866 0.5543 TD [(MS)-302.1(Method)]TJ 8.1197 0.5543 TD [(HLA-E)-302.8(peptide)-303.5(recogni-)]TJ 0 -1.1087 TD [(tion)-303.9(by)-299.1(T-cell)-302.9(assay)]TJ 13.1339 0.5543 TD [(Origin)-7993.6(Ref)]TJ /F6 1 Tf 0 6.9999 -6.9999 0 131.4708 75.7418 Tm 0.15294 0.15294 0.15294 scn [(28)-297.4(HLA-E)-297.1(peptides)-3355.8(7-11)]TJ 21.811 2.0329 TD [(-U373,)-299.3(DC,)-300.3(and)-308.1(A549)-297.7(cells)-304.5(uninfected)-304.5(and)]TJ 0 -1.3526 TD [(infected)-304.5(with)-303.4(the)-298(H37Rv)-303.9(strain)-299.5(of)-302.4(Mtb)]TJ 0 -1.3607 TD [(-PBMCs)-305.5(collected)-304.5(apheresis)-304.8(from)-300.8(healthy,)]TJ T* [(LTBI,)-304.7(and)-300(Mtb-infected)-304.6(adult)-303.6(donors)]TJ 19.5756 6.1067 TD (Af)Tj /F7 1 Tf 0.98 0 TD ()Tj /F6 1 Tf 0.5264 0 TD [(nity)-301.8(chromatography)]TJ -1.5064 -1.3526 TD [(with)-295.3(VLDLr,)-307.4(W6/32)-302.1(\(anti-)]TJ 0 -1.3607 TD [(pan)-300(HLA)-297.9(class)-307.2(I\),)-296.6(and)-308.1(3D12)]TJ 0 -1.3526 TD [(\(anti-HLA-E\))-300.8(antibodies)-306(to)]TJ 0 -1.3607 TD [(isolate)-297.7(soluble)-302(HLA-E)]TJ 0 -1.3526 TD [(peptides)-302.4(followed)-305.5(by)]TJ 0 -1.3607 TD (2D-HPLC)Tj 13.2259 7.4674 TD [(Nano)-301.9(LC)-303.3(coupled)-300.2(to)]TJ 0 -1.3607 TD [(Sciex)-304.6(5600)-303.2(Triple)]TJ 0 -1.3526 TD [(TOF)-305.7(in)-300.5(DDA)-301(\(for)]TJ 0 -1.3607 TD [(discovery\))-307(and)]TJ T* [(SWATH)-301.6(\(for)]TJ 0 -1.3526 TD (quanti)Tj /F7 1 Tf 2.5755 0 TD ()Tj /F6 1 Tf 0.5345 0 TD [(cation\))-303(modes)]TJ 7.3297 3.3935 TD (IFN-)Tj /F11 1 Tf 1.9519 0 TD (g)Tj /F6 1 Tf 0.7046 0 TD [(ELISPOT)-305.5(assay)]TJ 14.2302 4.0658 TD [(13)-297.4(Mtb)-305.4(source)-303.4(proteins)-304.8(\(i.e.,)]TJ 0 -1.3526 TD [(ftsH,)-300.4(iniB,)-300.5(lpqI,)-307.2(mpa,)-305.1(rplA,)]TJ 0 -1.3607 TD [(Rv0435c,)-306.2(Rv0634A,)-302.5(and)]TJ 0 -1.3526 TD [(Rv3479\))-297.5(and)-308.1(antigenic)-305.3(ESX)]TJ 0 -1.3607 TD [(operon)-301.5(proteins)-304.8(\(e.g.,)-299.6(EspA,)]TJ 0 -1.3526 TD [(ESAT-6,)-303.2(EsxG,)-304.2(EsxH)]TJ 0 -1.3607 TD [(and)-300(PE5.EsxG\))]TJ 13.9305 4.0739 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (56)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 -6.4469 TD (SEVENVSVNVHNPTG)Tj 11.1201 -3.3935 TD (15)Tj 10.6909 2.7132 TD [(-K562)-304.9(cell)-296(line)-306.7(transfected)-304.3(with)-303.4(HLA-)]TJ 0 -1.3526 TD (E*01:03)Tj 0 -1.3607 TD [(-NK)-298.2(cells)-304.5(cocultured)-303.5(with)-303.4(dendritic)-304.9(cells)-304.5(and)]TJ 0 -1.3526 TD [(loaded)-305.1(with)-295.3(the)-306.1(identi)]TJ /F7 1 Tf 8.8686 0 TD ()Tj /F6 1 Tf 0.5345 0 TD [(ed)-295.1(HLA-E)-305.2(peptides)]TJ -9.4031 -1.3607 TD [(to)-299.5(detect)-300.8(NK)-303.9(responses)]TJ 19.5756 6.1067 TD (Immunopeptidome)Tj 0 -1.3526 TD [(enrichment)-301.1(followed)-305.5(by)-297.4(size-)]TJ 0 -1.3607 TD [(exclusion)-298.5(chromatography)-311.1(to)]TJ T* [(isolate)-297.7(peptide-HLA-E)]TJ 0 -1.3526 TD [(complexes)-300.7(followed)-305.5(by)]TJ 0 -1.3607 TD [(elution)-296.8(by)-305.5(mild)-296.2(acetic)-304.3(acid)]TJ 13.2259 5.4345 TD [(Mass)-306.9(spectrometry)-305.9(in)]TJ 0 -1.3607 TD [(discovery)-309.9(mode)]TJ T* [(\(DDA\))-303.4(to)-299.5(identify)]TJ 0 -1.3526 TD [(HLA-E)-305.2(peptides)]TJ 10.4398 2.0329 TD (NK)Tj /F14 1 Tf 1.4092 0 TD ()Tj /F6 1 Tf 0.575 0 TD [(CTL-based)-297.5(T)-301.2(cell)-304.1(assay)]TJ 14.9024 0.6803 TD [(Human)-299.3(cytomegalovirus)]TJ 0 -1.3607 TD [(\(HCMV\))-300.9(pp65-protein)]TJ 13.9305 0.6803 TD (\()Tj 0.85098 0.19216 0.2 scn 0.3402 0 TD (57)Tj 0.15294 0.15294 0.15294 scn 0.9638 0 TD (\))Tj -97.1734 1.0205 TD (TSGSDSDEELVTTER)Tj 0 -3.3935 TD (DSDEELVTTERKTPR)Tj /F8 1 Tf 0 6.5001 -6.5001 0 238.6771 70.6394 Tm ()Tj /F6 1 Tf 0.4971 0 TD 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[(characterized)-305.1(or)-303.6(examined)-301.7(higher)-301.9(numbers)-302.7(of)-297(HLA-E-bound)-305.8(peptides,)-306.9(including)-304.5(Lampen)-300.4(et)-297.5(al.)-307.9(\()]TJ 0.85098 0.19216 0.2 scn 43.3304 0 TD (55)Tj 0.49804 0.49804 0.49412 scn 1.1096 0 TD [(\))-294(\(MS-based\),)-308.2(Joosten)-299.5(et)-305.6(al.)-299.8(\()]TJ 0.85098 0.19216 0.2 scn 12.9262 0 TD (53)Tj 0.49804 0.49804 0.49412 scn 1.1015 0 TD [(\))-302.1(\(non-MS\),)-302.2(and)]TJ -58.4676 -1.2878 TD [(other)-301.6(studies)-304.4(\(see)-300(also)]TJ 0.85098 0.19216 0.2 scn 10.4236 0 TD [(Supplementary)-305.1(Table)-303(S1)]TJ 0.49804 0.49804 0.49412 scn 10.8609 0 TD [(\).)-298(Venn)-300.3(diagrams)-305(were)-302.3(generated)-301(by)]TJ 0.85098 0.19216 0.2 scn 16.6761 0 TD (https://www.interactivenn.net/)Tj 0.49804 0.49804 0.49412 scn 14.2059 0 TD (\()Tj 0.85098 0.19216 0.2 scn 0.2754 0 TD (72)Tj 0.49804 0.49804 0.49412 scn 1.0772 0 TD [(\).)-298(The)-298.7(sequence)-306.5(motifs)-303.4(were)]TJ -53.5191 -1.2878 TD [(generated)-309.1(by)-296.8(Seq2Logo)-305.4(v.)-302.5(2.0)-299.1(\()]TJ 0.85098 0.19216 0.2 scn 14.1411 0 TD (https://services.healthtech.dtu.dk/services/Seq2Logo-2.0/)Tj 0.49804 0.49804 0.49412 scn 26.225 0 TD 0.3021 Tc (\)\()Tj 0.85098 0.19216 0.2 scn 0.8666 0 TD 0 Tc (73)Tj 0.49804 0.49804 0.49412 scn 1.061 0 TD [(\))-302.1(and)-300.2(GibbsCluster)-300.8(v.)-302.5(2.0)-299.1(\()]TJ 0.85098 0.19216 0.2 scn 11.687 0 TD (https://services.healthtech.)Tj -53.9807 -1.2878 TD (dtu.dk/services/GibbsCluster-2.0/)Tj 0.49804 0.49804 0.49412 scn 15.2831 0 TD 0.3021 Tc (\)\()Tj 0.85098 0.19216 0.2 scn 0.8666 0 TD 0 Tc (74)Tj 0.49804 0.49804 0.49412 scn 1.1015 0 TD [(\))-302.1(software)-300.5(tools.)-302.1(The)-306.8(bar)-294.3(graphs)-306(were)-302.3(generated)-301(by)-304.9(GraphPad)-296.4(Prism)-307.6(v.)-294.4(9.3.1.)]TJ /F1 1 Tf -18.8548 75.8565 TD [(Weitzen)-302.7(et)-300.8(al.)]TJ 57.0665 0 TD (10.3389/fimmu.2024.1442783)Tj -57.0665 -109.3302 TD [(Frontiers)-304.6(in)]TJ 0.85098 0.19216 0.2 scn 5.5074 0 TD (Immunology)Tj 58.8564 0 TD (frontiersin.org)Tj 0.15294 0.15294 0.15294 scn -29.4647 0 TD (10)Tj ET endstream endobj 197 0 obj<>stream H=hTo"b +M%$QS)Z ڊVD!b-&iP|A0:̉jrs2+6묳kI)-eR{?GJ)`9={GW{:?|X سgOtR3?5==cp3tR#xe1TTw`RxbXD=,@j7o1b=|0,.955СCE1,\.=/@Gzݚ5kB۷'J|StW,o޽{w#1k^O)8<@H)6BǏ7olfbx``q7:Ɠ'Ow1f|ر>]6dj6I)@g8qDhch9rl)NRJsnnP(66ljʉRTV7n,rYS6nܘY6::7dyYkٹskbyMA&''C;v,|y=cl/exrʕ+Ž+>8@gH)j_~~ڵkuRjŗ!`kYJ+JU t{,nj/N)ÇZhzE. \,ܿ?,bjju7)Fpc=z'׿~Z*Br"tZiӦgž}b1bwwwZ7O)j%Ƙţyo\*2/y.}ӚC .,3gdT*Kܹs',R,dOr:dB]v-zbZ.?jRj|.̂o޼'|5V2@~OJ)/UpQ1'S2ʨ^5!BM(J#Rv"#ԍ^DÅQT"A;EEQh#![65{8~_w1l]zzgߋi:'?Ȯ_={}ݻw|?<̈>l5t޽{\ &%% :_~ᱱSLY~͛7XuQQQOQQQmmm/^())IKK}zKKKzˆ{^T@ı(ޛ?.^d &t~~~mmiBe'UUU+W;vldddhhȑ#sԩ.? 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[(c)12.8(i)0(t)20.3(y)-291.6(fo)16.2(r)-291.1(i)0(s)19.3(o)15(la)19.3(ti)20.3(o)15(n)]TJ -20.3339 -1.3858 TD [(of)-235.8(p)16.1(H)0(L)18.6(A)13(-E)-236.8(a)15(n)0(d)-236.6(M)0(S)-234.4(i)14.3(ns)16.8(tr)23.5(um)21.8(e)12.9(n)0(t)17.8(a)0(t)20.9(i)14.3(on)-237.7(se)24.1(ns)23.1(it)20.3(iv)18.8(i)14.3(t)0(y)-235.3(c)12.8(on)20.5(ti)20.3(nu)21.6(e)-245.4(t)0(o)-231.1(i)0(m)20(p)0(r)21(o)15(ve)17.3(,)]TJ 0 -1.3921 TD [(th)20.4(e)12.9(r)0(e)-429.4(i)14.3(s)-442.3(c)12.9(on)20.5(si)19.3(de)22.6(ra)19.9(b)15(l)0(e)-430.1(p)0(o)24.7(t)0(e)18.8(n)0(t)24.1(i)0(a)16.7(l)-436.6(f)13.8(o)0(r)-427.3(h)0(i)22.5(g)0(h)18.9(-)13.8(th)20.4(ro)19.9(ug)20.5(h)14.5(p)0(u)19.5(t)-441.3(di)24.1(sc)17.8(o)15(v)0(e)17.3(r)0(y)-425.2(a)0(n)20.5(d)]TJ 0 -1.3858 TD [(va)19.4(li)25(da)18.4(ti)20.3(o)15(n)-202.3(o)15(f)-200.3(HL)24.9(A-)20.5(E)-200.2(v)0(i)25.1(r)0(a)19.9(l)-197.2(ep)16.3(i)14.3(t)0(o)20.9(p)0(e)22.6(s)-202.9(a)15(n)0(d)-192.5(t)0(u)22(m)0(o)20.7(r)-196.6(a)15(n)0(t)17.8(i)14.3(ge)17.3(ns)23.1(.)-197.7(I)0(n)-190.6(t)0(h)20.4(e)-195(co)21.5(nt)17.8(e)12.8(x)0(t)]TJ 0 -1.3921 TD [(of)-468.8(a)15(n)0(t)17.8(i)14.3(-c)20.4(an)20.5(c)12.8(e)0(r)-473.5(i)14.3(mm)17.7(u)16.1(n)0(o)20.5(t)0(h)20.4(e)12.8(ra)19.9(pe)16.3(u)16.1(t)0(i)20.3(c)0(s)17.8(,)-474.9(tu)22(mo)20.7(rs)-468.8(o)15(f)0(t)19.8(e)0(n)-466.6(m)0(a)20.7(n)0(i)19.9(p)0(u)19.5(l)0(a)25.6(t)0(e)-472.5(t)0(h)26.7(e)]TJ 0 -1.3858 TD [(an)20.5(ti)20.3(ge)23.6(n)-328.3(p)0(r)21(o)0(c)21.5(e)0(s)17.8(s)0(i)25.6(n)0(g)-323.8(a)15(nd)-324.8(p)16.1(r)0(e)17.8(s)0(e)24.1(n)0(t)17.8(a)0(t)20.9(i)14.3(on)-319.6(pa)18.4(th)26.7(wa)17.9(y)-329.4(t)0(o)-319.3(e)12.9(va)19.4(de)-317.5(th)20.4(e)-327.3(i)0(m)20(m)0(u)21.8(n)0(e)]TJ 0 -1.3921 TD 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[(te)18.8(c)12.8(h)0(n)20(o)15(lo)19.3(gi)25.1(es)17.8(,)-462.3(w)15.6(e)-459.6(m)0(a)20.7(y)-461.7(un)21.6(lo)19.3(c)12.8(k)-455.9(ne)18.4(w)-456.9(a)0(v)19.4(e)0(n)18.4(u)0(e)22.6(s)-461.2(f)13.8(o)0(r)-452.5(i)14.3(mp)21.8(ro)19.9(vi)18.8(ng)-449.8(p)16.1(a)0(t)20.9(i)0(e)20.9(n)0(t)]TJ 0 -1.3921 TD [(ou)18.4(tc)25.1(om)20.7(e)12.9(s)-291.1(ac)21.5(ro)19.9(ss)-279.9(a)-287.4(d)0(i)17.8(v)17.1(er)17.8(se)-278.3(ra)19.9(ng)22.6(e)-289.5(o)0(f)-279.9(d)0(i)24.1(s)0(e)17.8(a)0(s)19.9(e)12.8(s.)]TJ /F1 1 Tf 13.5 0 0 13.5 304.8944 755.5463 Tm 0.85098 0.19216 0.2 scn [(Author)-302.2(contributions)]TJ /F6 1 Tf 9 0 0 9 318.8409 730.5447 Tm 0.15294 0.15294 0.15294 scn -0.0229 Tc [(MW)-6.1(:)-434.8(W)-6.1(riting)]TJ /F8 1 Tf 5.8835 0 TD 0 Tc ()Tj /F6 1 Tf 0.926 0 TD -0.021 Tc [(original)-432.4(draf)5.4(t.)-432.9(MS:)-439.2(F)1.4(ormal)-432.4(a)0.3(nalysis,)-432.9(Writing)]TJ /F8 1 Tf 17.915 0 TD 0 Tc ()Tj /F6 1 Tf -26.274 -1.3858 TD -0.0238 Tc [(rev)-6.7(i)-3.2(ew)-411.4(&)-411.9(e)-4.7(diting.)-416.8(S)-3(K:)-410.5(Wr)-6.3(it)-5.3(ing)]TJ /F8 1 Tf 12.2772 0 TD 0 Tc ()Tj /F6 1 Tf 0.9008 0 TD -0.0226 Tc [(review)-410.2(&)-410.7(e)-3.5(diting.)-409.3(E)-2.3(C:)-415.6(Supervision,)]TJ -13.178 -1.3921 TD -0.0215 Tc [(Funding)-300.5(a)6.1(c)-2.4(quisition,)-301.1(Conceptualizatio)6.1(n,)-301.1(Writi)5.4(n)-3.3(g)]TJ /F8 1 Tf 18.8347 0 TD 0 Tc ()Tj /F6 1 Tf 0.7874 0 TD -0.0226 Tc [(review)-303.1(&)-303.6(e)2.8(dit)-4.1(i)4.3(n)-4.4(g)0.8(.)]TJ /F1 1 Tf 13.5 0 0 13.5 304.8944 668.0692 Tm 0.85098 0.19216 0.2 scn 0 Tc (Funding)Tj /F6 1 Tf 9 0 0 9 318.8409 643.0676 Tm 0.15294 0.15294 0.15294 scn [(The)-215.4(author\(s\))-214.1(declare)-217.2(that)]TJ /F7 1 Tf 10.5134 0 TD ()Tj /F6 1 Tf 0.5291 0 TD [(nancial)-217.2(support)-217.1(was)-210.2(received)-217.4(for)-211.9(the)]TJ -12.5921 -1.3921 TD [(research,)-297.6(authorship,)-303.3(and/or)-301.8(publication)-300.3(of)-292.5(this)-300.5(article.)-297.6(This)-297.1(article)]TJ 0 -1.3858 TD [(was)-266.9(supported)-270(by)-270.3(a)-268.5(startup)-271.3(package)-272.6(provided)-271.9(by)-270.3(Yale)-268.8(University)-276.3(to)]TJ 0 -1.3921 TD [(establish)-298.8(the)-300.6(Caron)-305.1(laboratory.)]TJ /F1 1 Tf 13.5 0 0 13.5 304.8944 571.0676 Tm 0.85098 0.19216 0.2 scn (Con)Tj /F18 1 Tf 1.9234 0 TD ()Tj /F1 1 Tf 0.5963 0 TD [(ict)-302.2(of)-300.2(interest)]TJ /F6 1 Tf 9 0 0 9 318.8409 546.066 Tm 0.15294 0.15294 0.15294 scn [(The)-448.4(authors)-446.5(declare)-450.3(that)-443.7(the)-445.5(research)-452.6(was)-443.2(conducted)-453.7(in)-446.2(the)]TJ -1.5496 -1.3921 TD [(absence)-299(of)-298.8(any)-302.6(commercial)-299.6(or)]TJ /F7 1 Tf 12.3654 0 TD ()Tj /F6 1 Tf 0.5291 0 TD [(nancial)-299.1(relationships)-308.3(that)-298.9(could)-301.1(be)]TJ -12.8945 -1.3858 TD [(construed)-302.6(as)-301.4(a)-300(potential)-301.9(con)]TJ /F7 1 Tf 11.3764 0 TD ()Tj /F6 1 Tf 0.5291 0 TD [(ict)-300.7(of)-298.8(interest.)]TJ /F1 1 Tf 13.5 0 0 13.5 304.8944 489.5432 Tm 0.85098 0.19216 0.2 scn (Publisher)Tj /F2 1 Tf 4.2583 0 TD ()Tj /F1 1 Tf 0.2184 0 TD [(s)-300.5(note)]TJ /F6 1 Tf 9 0 0 9 318.8409 464.5417 Tm 0.15294 0.15294 0.15294 scn [(All)-211.4(claims)-213.7(expressed)-215(in)-213.2(this)-206(article)-213.2(are)-212.9(solely)-212.4(those)-211.4(of)-210.6(the)-206.1(authors)]TJ -1.5496 -1.3858 TD 0.0497 Tc [(and)-545.3(d)-3.5(o)-546.4(n)-1.4(ot)-549.1(necessarily)-544.3(represent)-549.1(t)5.2(hose)-554.8(of)-547.5(their)-550.1(a)1.7(f)]TJ /F7 1 Tf 23.7669 0 TD 0 Tc ()Tj /F6 1 Tf 0.5858 0 TD 0.049 Tc (liated)Tj -24.3528 -1.3921 TD 0.0168 Tc [(o)6.6(r)-3.5(ganizations,)-521.1(or)-513.7(those)-518.4(o)0.3(f)-517.4(t)3.8(he)-518.4(publisher,)-521.1(t)3.8(he)-518.4(editors)-513.7(a)0.3(nd)-515.2(the)]TJ 0 -1.3858 TD 0 Tc [(reviewers.)-424.9(Any)-430.5(product)-423.3(that)-424.8(may)-428.4(be)-425.7(evaluated)-426.6(in)-427.3(this)-426.4(article,)-429.9(or)]TJ 0 -1.3921 TD [(claim)-313.2(that)-311.5(may)-315(be)-312.3(made)-315.8(by)-314.4(its)-314.9(manufacturer,)-321(is)-314.6(not)-313.7(guaranteed)-319.7(or)]TJ 0 -1.3858 TD [(endorsed)-302.3(by)-301.8(the)-300.6(publisher.)]TJ /F1 1 Tf 13.5 0 0 13.5 304.8944 370.5448 Tm 0.85098 0.19216 0.2 scn [(Supplementary)-303.7(material)]TJ /F6 1 Tf 9 0 0 9 318.8409 345.5432 Tm 0.15294 0.15294 0.15294 scn -0.0217 Tc [(The)-235.6(S)5.4(u)-5.6(pplementary)-231.4(Material)-231.5(fo)5.9(r)-230.9(t)-3.2(hi)5.2(s)-230.9(a)-0.4(rticle)-229.3(can)-230.3(b)-0.4(e)-229.3(f)-1.6(o)5.9(u)-5.6(nd)-232.4(online)-229.3(at:)]TJ 0.85098 0.19216 0.2 scn -1.5496 -1.3858 TD 0 Tc [(htt)-11.5(p)-9.1(s)0(:)-10.1(/)0(/)-11.1(w)0(w)-12.9(w)0(.)-12.1(fro)-16.7(nt)-7.4(ie)-10.6(r)-7.7(s)-7.7(i)0(n)-11.6(.or)-14.1(g)-8.1(/)0(a)-9.5(r)-7.7(ti)-11.2(cl)-8(es)-13.7(/1)-11.6(0.)-8.5(33)-12.1(89)-12.1(/)]TJ /F7 1 Tf 17.9906 0 TD ()Tj /F6 1 Tf 0.5354 0 TD [(m)-6.9(m)0(u)-9.7(.)0(2)-8.5(0)0(2)-12.1(4)0(.)-14.8(144)-11.8(27)-12.1(83)-12.1(/)]TJ 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[(25.)-500.6(Wa)28.1(lt)33.7(er)26.8(s)-300.1(L)0(C)28.8(,)-300.3(Ro)28.7(z)19.1(b)0(e)25.1(s)15.7(ky)-284.1(D)16.3(,)-300.3(Ha)30.7(r)15.7(l)0(o)29.2(s)-300.1(K,)-282.3(Qu)27.9(a)15.8(s)0(t)26.2(e)0(l)-281.6(M)0(,)-282(S)0(u)24.2(n)-293.2(H,)-285.5(Sp)24.2(r)15.7(i)17(ng)27.1(er)-281(S,)-285.9(et)-286.3(a)15.8(l)0(.)-277.1(P)0(r)27(i)0(m)27.2(a)0(r)23.5(y)]TJ -0.9314 -1.142 TD [(a)15.8(n)0(d)-275.4(s)15.7(ec)22.1(on)28.6(d)17.9(a)0(r)23.5(y)-287.1(f)15.6(u)0(n)24.3(c)19.1(ti)27.4(on)28.6(s)-292(o)0(f)-270(H)0(L)27.5(A)0(-)28.6(E)-288.4(a)15.8(r)0(e)-272.9(d)0(e)20.8(t)18.5(er)26.8(mi)27.2(ne)25.6(d)-289.9(b)0(y)-273.1(s)15.7(t)18.5(a)0(b)21.8(i)17(li)32.1(ty)-276.7(a)15.8(n)0(d)-275.4(c)0(o)25.1(n)0(f)30.2(o)0(r)29.8(m)0(a)26(t)0(i)27.4(o)0(n)-271.1(o)0(f)-278.1(t)18.5(he)]TJ T* [(pe)28.9(pt)28.3(id)26.8(e-)24.9(bo)28.1(u)17.9(n)0(d)-275.4(c)0(o)25.1(m)18.3(pl)24.9(ex)25.5(e)19.1(s)0(.)]TJ /F15 1 Tf 10.5532 0 TD [(C)17.6(e)0(l)26.6(l)-287.4(Re)24.2(p)]TJ /F6 1 Tf 3.1668 0 TD [(.)-284.1(\()0(2)20.2(0)17.4(2)17.3(2)0(\))-271.4(3)0(9)26.6(:)15.5(11)26.6(09)26.6(5)17.3(9)0(.)-274.9(d)0(o)23.8(i)17(:)-284.1(1)0(0)26.6(.)15.5(10)26.6(16)26.6(/j)29.3(.)15.5(c)0(e)22.1(l)0(r)30.9(e)0(p)28.9(.)0(2)24.8(0)17.3(22)26.6(.)15.5(1)0(1)26.6(0)0(9)26.6(5)17.3(9)]TJ -12.7885 -1.5712 TD [(26.)-500.6(Vaurs)-250.3(J,)-253.7(Douchin)-253(G,)-248.4(Echasserieau)-258.6(K,)-249.9(Oger)-258.4(R,)-245.2(Jouand)-253.9(N,)-257.9(Fortun)-249.7(A,)-253.2(et)-245.8(al.)-253.2(A)-252.5(novel)]TJ -0.9314 -1.142 TD [(and)-235.2(ef)]TJ /F7 1 Tf 2.3811 0 TD ()Tj /F6 1 Tf 0.5264 0 TD [(cient)-235.6(approach)-235.6(to)-234.7(high-throughput)-234.8(production)-238.4(of)-229.5(HLA-E/peptide)-244.7(monomer)-234.3(for)]TJ -2.9076 -1.142 TD [(T-cell)-299.9(epitope)-305.7(screening.)]TJ /F15 1 Tf 10.1401 0 TD [(Sci)-298.2(Rep)]TJ /F6 1 Tf 2.8752 0 TD [(.)-300.3(\(2021\))-297.6(11:17234.)-309.3(doi:)-300(10.1038/s41598-021-96560-9)]TJ -12.0839 -1.5712 TD [(27.)-500.6(Braud)-297.4(VM,)-305.8(Allan)-305.3(DSJ,)-295.9(Wilson)-308.1(D,)-300.3(McMichael)-306.9(AJ.)-295.7(TAP-)-301.9(and)-308.1(tapasin-dependent)]TJ -0.9314 -1.142 TD [(HLA-E)-216.1(surface)-213(expression)-217.3(correlates)-220.7(with)-206.2(the)-217(binding)-216.4(of)-213.3(an)-212.6(MHC)-209.8(class)-218.1(I)-209.6(leader)-213.1(peptide.)]TJ /F15 1 Tf T* [(Curr)-302.8(Biol)]TJ /F6 1 Tf 3.7337 0 TD [(.)-308.4(\(1998\))-297.6(8:1)]TJ /F8 1 Tf 4.6003 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(10.)-306.2(doi:)-300(10.1016/S0960-9822\(98\)70014-4)]TJ -7.9129 -1.5793 TD [(28.)-500.6(Sensi)-267.4(M,)-265.8(Pietra)-275.1(G,)-264.6(Molla)-269.8(A,)-269.4(Nicolini)-272.9(G,)-264.6(Vegetti)-275.3(C,)-268(Bersani)-274.7(I,)-267(et)-262(al.)-269.4(Peptides)-276.6(with)]TJ -0.9314 -1.142 TD [(dual)-314(binding)-321.7(speci)]TJ /F7 1 Tf 7.4269 0 TD ()Tj /F6 1 Tf 0.5264 0 TD [(city)-321.5(for)-311(HLA-A2)-316.8(and)-324.3(HLA-E)-313.3(are)-321.9(encoded)-315.7(by)-313.6(alternatively)-325(spliced)]TJ -7.9533 -1.142 TD [(isoforms)-311.2(of)-302.4(the)-306.1(antioxidant)-307.8(enzyme)-310.3(peroxiredoxin)-307.4(5.)]TJ /F15 1 Tf 21.568 0 TD [(Int)-308.1(Immunol)]TJ /F6 1 Tf 5.1429 0 TD [(.)-300.3(\(2009\))-305.7(21:257)]TJ /F8 1 Tf 6.042 0 TD ()Tj /F6 1 Tf 0.5103 0 TD (68.)Tj -33.2631 -1.142 TD [(doi:)-300(10.1093/intimm/dxn141)]TJ 0.9314 -1.5712 TD [(29.)-500.6(Pietra)-210.3(G,)-207.9(Romagnani)-211(C,)-203.2(Mazzarino)-214.8(P,)-208.1(Falco)-209.6(M,)-209.1(Millo)-203.8(E,)-208.1(Moretta)-211.9(A,)-204.6(et)-213.4(al.)-204.6(HLA-E-)]TJ -0.9314 -1.142 TD [(restricted)-228.5(recognition)-220.6(of)-221.4(cytomegalovirus-derived)-228.3(peptides)-229.5(by)-224.5(human)-220.5(CD8+)-220.6(cytolytic)-226(T)]TJ T* (lymphocytes.)Tj /F15 1 Tf 5.4669 0 TD [(Proc)-217.2(Natl)-224.9(Acad)-215.4(Sci)]TJ /F6 1 Tf 6.9999 0 0 6.9999 138.7842 315.3826 Tm [(.)-211.3(\(2003\))-224.7(100:10896)]TJ /F8 1 Tf 7.3054 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(901.)-224(doi:)-219.1(10.1073/pnas.1834449100)]TJ -19.6242 -1.5712 TD [(30.)-500.6(Mazzarino)-247.2(P,)-248.6(Pietra)-242.7(G,)-248.4(Vacca)-245.5(P,)-248.6(Falco)-242(M,)-249.6(Colau)-245(D,)-243.6(Coulie)-249(P,)-240.5(et)-245.8(al.)-245.1(Identi)]TJ /F7 1 Tf 30.5175 0 TD ()Tj /F6 1 Tf 0.5345 0 TD (cation)Tj -31.9835 -1.142 TD [(of)-237.6(ef)59.1(fector-memory)-239.6(CMV-speci)]TJ /F7 1 Tf 12.497 0 TD ()Tj /F6 1 Tf 0.5264 0 TD [(c)-240(T)-333.6(lymphocytes)-237.6(that)-242.2(kill)-233.2(CMV-infected)-247.6(_target)-239.9(cells)-231.6(in)]TJ -13.0234 -1.142 TD [(an)-350.3(HLA-E-restricted)-366(fashion.)]TJ /F15 1 Tf 12.1649 0 TD [(Eur)-351(J)-352.1(Immunol)]TJ /F6 1 Tf 6.131 0 TD [(.)-348.9(\(2005\))-354.3(35:3240)]TJ /F8 1 Tf 6.617 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(7.)-355.9(doi:)-348.6(10.1002/\(ISSN\))]TJ -25.4232 -1.142 TD (1521-4141)Tj 0.9314 -1.5712 TD [(31.)-500.6(Hoare)-293(HL,)-305.2(Sullivan)-298.7(LC,)-295.9(Pietra)-299.4(G,)-297(Clements)-300.8(CS,)-294.1(Lee)-297.4(EJ,)-299.2(Ely)-301.3(LK,)-294(et)-302.5(al.)-293.7(Structural)]TJ -0.9314 -1.1501 TD [(basis)-350.9(for)-359.6(a)-348.6(major)-358.4(histocompatibility)-355.1(complex)-359.9(class)-355.8(Ib)]TJ /F8 1 Tf 21.649 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(restricted)-358.1(T)-349.8(cell)-352.7(response.)]TJ /F15 1 Tf 10.7961 0 TD (Nat)Tj -32.9554 -1.142 TD (Immunol)Tj /F6 1 Tf 3.6689 0 TD [(.)-300.3(\(2006\))-297.6(7:256)]TJ /F8 1 Tf 5.556 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(64.)-298.1(doi:)-308.1(10.1038/ni1312)]TJ -8.8038 -1.5712 TD [(32.)-500.6(Ulbrecht)-331.1(M,)-322.5(Hofmeister)-334.1(V,)-332.3(Yksekdag)-331(G,)-329.4(Ellwart)-332.9(JW,)-330.5(Hengel)-325.8(H,)-334.1(Momburg)-326.5(F,)]TJ -0.9314 -1.142 TD [(et)-391.6(al.)-399(HCMV)-395.6(glycoprotein)-393(US6)-397.4(mediated)-391(inhibition)-402.4(of)-391.5(TAP)-396.7(does)-394.9(not)-390.2(af)63.9(fect)-397.3(HLA-E)]TJ T* [(dependent)-246.4(protection)-244.5(of)-237.6(K-562)-240.1(cells)-247.8(from)-236(NK)-247.2(cell)-239.3(lysis.)]TJ /F15 1 Tf 22.0539 0 TD [(Hum)-241.1(Immunol)]TJ /F6 1 Tf 5.961 0 TD [(.)-243.6(\(2003\))-240.9(64:231)]TJ /F8 1 Tf 5.9205 0 TD ()Tj /F6 1 Tf -33.9354 -1.142 TD [(7.)-299.2(doi:)-300(10.1016/S0198-8859\(02\)00788-7)]TJ 0.9314 -1.5712 TD [(33.)-500.6(Wooden)-458.9(SL,)-451.5(Kalb)-455.3(SR,)-457.5(Cotter)-456.7(RJ,)-457.8(Soloski)-457.3(MJ.)-462.1(Cutting)-451.8(edge:)-458.5(HLA-E)-459.1(binds)-455.4(a)]TJ -0.9314 -1.142 TD [(pe)-11.6(pti)-11.4(de)-505.7(de)-11.6(riv)-11.4(ed)-505.7(fro)-11.3(m)-508.2(t)0(h)-9.3(e)-507.3(ATP-)-10.3(b)-10.2(indi)-14.6(ng)-507.5(cas)-14.1(set)-11.4(te)-505(tra)-14.7(ns)-10.2(port)-14.9(er)-507.8(mul)-13.6(tid)-11.4(rug)-512.7(res)-14.2(ista)-13.9(nce)-12(-)]TJ T* 0.016 Tc [(as)16(s)-9.2(o)-2.3(ciated)-508.8(pro)5.8(t)2.1(ein)-512.1(7)-509.3(aS)6.2(nd)-508.8(inhibi)16(t)-13.2(s)-510.9(NK)-508.6(cel)6.8(l)-1.3(-)5.5(m)1.9(edi)16(a)-7.8(ted)-516.9(l)6.8(ysi)16(s)-7.9(1.)]TJ /F15 1 Tf 26.4113 0 TD 0.0123 Tc [(J)-518(I)-3(mmu)-7.4(no)-5.7(l)]TJ /F6 1 Tf 4.6084 0 TD 0.015 Tc [(.)-512.1(\()1.6(2005\))]TJ -31.0197 -1.142 TD 0 Tc (175:1383)Tj /F8 1 Tf 3.5717 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(7.)-299.2(doi:)-308.1(10.4049/jimmunol.175.3.1383)]TJ -3.1506 -1.717 TD [(34.)-500.6(Lee)-386.4(N,)-395.6(Goodlett)-389.7(DR,)-390.9(Ishitani)-398(A,)-390.9(Marquardt)-391(H,)-398.9(Geraghty)-391.9(DE.)-386.2(HLA-E)-394.3(surface)]TJ -0.9314 -1.142 TD [(expression)-265.9(depends)-266.7(on)-263(binding)-265(of)-261.9(TAP-dependent)-273(peptides)-270(derived)-261.4(from)-268.4(certain)-260.7(HLA)]TJ T* 0.0556 Tc [(class)-552.3(I)-559(si)7.8(g)-4.7(n)5.4(al)-552.9(sequences1.)]TJ /F15 1 Tf 12.6184 0 TD 0.0528 Tc [(J)-558.5(I)-3(mmun)-7.4(o)2.5(l)]TJ /F6 1 Tf 4.9648 0 TD 0.0555 Tc [(.)-560.7(\()1.6(1998)8.1(\))-557.2(160:)6.2(4951)]TJ /F8 1 Tf 8.3259 0 TD 0 Tc ()Tj /F6 1 Tf 0.5669 0 TD 0.0553 Tc [(60.)-552.8(doi:)-552.8(10.4049/)]TJ -26.4761 -1.142 TD 0 Tc (jimmunol.160.10.4951)Tj 0.9314 -1.5712 TD [(35.)-500.6(Allard)-270.1(M,)-273.9(Tonnerre)-277.5(P,)-272.9(Nedellec)-278.4(S,)-269.7(Oger)-274.6(R,)-277.6(Morice)-269.1(A,)-277.5(Guilloux)-274(Y,)-266.6(et)-278.2(al.)-269.4(HLA-E-)]TJ -0.9314 -1.1501 TD [(restricted)-325.7(cross-recognition)-320.8(of)-326.7(allogeneic)-316.2(endothelial)-323.2(cells)-320.7(by)-321.7(CMV-associated)-325(CD8)-322.8(T)]TJ 0 -1.142 TD [(cells:)-459.1(A)-455(potential)-465(risk)-458.7(factor)-460(following)-452.3(transplantation.)]TJ /F15 1 Tf 23.1716 0 TD [(PloS)-461.7(One)]TJ /F6 1 Tf 3.8066 0 TD [(.)-454.2(\(2012\))-459.5(7:e50951.)]TJ -26.9782 -1.142 TD [(doi:)-300(10.1371/journal.pone.0050951)]TJ 0.9314 -1.5712 TD [(36.)-500.6(To)28.7(m)18.3(a)0(s)23.5(e)0(c)-350.5(P)0(,)-353.9(B)0(r)23.7(a)15.8(ud)-353.1(VM)26.9(,)-373.2(R)0(i)31.7(c)0(k)22.2(a)15.8(r)15.7(d)0(s)-355.2(C)16.2(,)-373.2(P)19.4(ow)26.9(el)26.2(l)-365.5(M)0(B)26.3(,)-365.1(Mc)29.4(Sh)27.1(ar)23.5(r)15.7(y)-368.1(B)16.1(P)0(,)-353.9(G)0(a)27.3(d)0(o)23.8(l)0(a)-349.7(S)0(,)-358.8(e)19.1(t)-370.2(a)15.8(l.)]TJ -0.9314 -1.142 TD [(Su)24.2(r)15.7(f)15.6(a)15.8(c)0(e)-366.7(e)0(x)25.5(p)17.9(re)26.8(ss)23.4(i)17(o)0(n)-368.3(o)0(f)-359.1(H)0(L)27.5(A)0(-)28.6(E)0(,)-362(a)0(n)-366.5(i)0(n)23.5(h)20.8(ib)23(i)17(t)0(o)24.5(r)-373(of)-367.2(na)30.4(tu)28.3(ra)23.5(l)-373.6(k)0(i)28.2(l)0(l)30.2(e)0(r)-370.1(c)19.1(el)26.2(ls)22.8(,)-373.2(e)0(n)25.6(h)0(a)28.5(n)0(c)25.6(e)19.1(d)-379(b)0(y)-362.2(h)0(u)22.4(m)18.3(an)]TJ T* [(cy)23.6(t)18.5(o)0(m)24.2(e)19.1(ga)28.4(lo)29.2(vi)21.5(r)15.7(u)17.9(s)-251.5(g)20.6(pU)26(L4)30(0.)]TJ /F15 1 Tf 10.1401 0 TD [(Sc)31.2(ie)25.2(nc)31.3(e)]TJ /F6 1 Tf 2.7051 0 TD [(.)-243.6(\()19(20)26.6(00)26.6(\))-248.2(2)17.4(8)17.3(7)0(:)24.8(1)17.4(03)26.6(1)]TJ /F8 1 Tf 6.698 0 TD ()Tj /F6 1 Tf 0.494 0 TD [(3)17.3(.)-243.7(do)23.8(i)17(:)-243.7(10)26.6(.)15.5(1)0(1)26.6(2)0(6)26.6(/)0(s)30(c)0(i)28.1(e)0(n)25.6(c)0(e)22.1(.)15.5(2)17.3(8)0(7)26.6(.)15.5(54)26.6(55)26.6(.)15.5(1)0(0)26.6(3)17.3(1)]TJ 17.3564 96.4283 TD 0.5352 Tc [(3)512.1(7)520.2(.)8.1(H)517.7(e)513.8(a)518.7(t)513.2(l)517.9(e)513.8(y)-3(S)509.1(L)515.5(,P)514.1(i)511.7(e)513.8(t)513.2(r)518.5(a)-7.8(G)514.3(,L)515.5(i)511.7(n)-1(J)508.9(,)0(W)515.1(i)511.7(d)512.6(j)517.9(a)510.6(j)517.9(a)-7.8(J)517(M)513.1(L)515.5(,H)509.6(a)518.7(r)510.4(p)520.7(u)512.5(r)-7.9(C)519(M)513.1(,)-8.1(L)515.5(e)513.9(s)518.5(t)513.2(e)513.9(r)0.2(S)509.2(,)0(e)513.8(t)-5.1(a)510.6(l)517.9(.)]TJ -0.9233 -1.142 TD 0 Tc [(Polymorphism)-416.9(in)-422(human)-423(cytomegalovirus)-418(UL40)-422.4(impacts)-420.3(on)-425(recognition)-415(of)-423.9(human)]TJ T* [(leukocyte)-250.3(antigen-E)-259.7(\(HLA-E\))-259.1(by)-256.9(natural)-259.1(killer)-255(cells*.)]TJ /F15 1 Tf 21.163 0 TD [(J)-254.9(Biol)-257.1(Chem)]TJ /F6 1 Tf 4.6813 0 TD [(.)-259.8(\(2013\))-249(288:8679)]TJ /F8 1 Tf 6.9086 0 TD ()Tj /F6 1 Tf 0.5102 0 TD (90.)Tj -33.2631 -1.142 TD [(doi:)-300(10.1074/jbc.M112.409672)]TJ 0.9233 -1.5712 TD [(38.)-500.6(Garc)]TJ /F20 1 Tf 3.6041 0 TD ()Tj /F19 1 Tf 0.2916 0.0405 TD ()Tj /F6 1 Tf -0.0324 -0.0405 TD [(a)-445.8(P)0(,)-443(Llano)-446.2(M,)-452.1(Heredia)-447.4(A,)-439.5(Willberg)-454.1(CB,)-438.2(Caparro)]TJ /F19 1 Tf 20.345 0.0405 TD ()Tj /F6 1 Tf 0.0891 -0.0405 TD [(s)-437.8(E)0(,)-451.1(Aparicio)-443.7(P,)-451.1(et)-440.2(al.)]TJ -25.2207 -1.142 TD [(Human)-202.1(T)-204(cell)-206.9(receptor-mediated)-205.5(recognition)-212.5(of)-205.2(HLA-E.)]TJ /F15 1 Tf 22.2483 0 TD [(Eur)-205.2(J)-206.4(Immunol)]TJ /F6 1 Tf 5.8314 0 TD [(.)-211.3(\(2002\))-200.4(32:936)]TJ /F8 1 Tf 5.8476 0 TD ()Tj /F6 1 Tf -33.9273 -1.142 TD [(44.)-298.1(doi:)-300(10.1002/\(ISSN\)1521-4141)]TJ 0.9233 -1.5712 TD [(39.)-500.6(Schulte)-255.7(D,)-251.7(Vogel)-254.6(M,)-249.6(Langhans)-258.3(B,)-251.9(Krmer)-253.6(B,)-251.9(Krner)-251(C,)-251.8(Nischalke)-261.1(HD,)-244.9(et)-253.9(al.)-253.2(The)]TJ -0.9233 -1.1501 TD [(HLA-ER/HLA-ER)-477.9(genotype)-471.1(af)63.9(fects)-478.8(the)-476.1(natural)-469.7(course)-481.5(of)-472.5(hepatitis)-471.4(C)-477.9(virus)-471.9(\(HCV\))]TJ 0 -1.142 TD [(infection)-457.9(and)-453.9(is)-461.3(associated)-454.8(with)-457.3(HLA-E)]TJ /F8 1 Tf 16.6194 0 TD ()Tj /F6 1 Tf 0.5103 0 TD [(restricted)-455.2(recognition)-463.6(of)-456.3(an)-455.6(HCV-derived)]TJ -17.1297 -1.142 TD [(pept)-15.8(ide)-512.9(b)-10.2(y)-505.8(int)-14.7(erf)-14.3(eron)-17.5(-)]TJ /F11 1 Tf 9.3545 0 TD (g)Tj /F8 1 Tf 0.405 0 TD ()Tj /F6 1 Tf 0.5183 0 TD [(sec)-10.8(re)-13.7(tin)-14.7(g)-505.8(h)0(u)-10(man)-518.3(CD8+)-520.2(T)-503.7(c)0(e)-10.3(l)-9.2(ls.)]TJ /F15 1 Tf 13.971 0 TD [(J)-506(I)0(n)-10.7(fec)-12.9(t)-507.9(D)0(i)-13(sea)-14.1(ses)]TJ /F6 1 Tf 6.8357 0 TD [(.)-510.9(\()0(2)-12.2(009)-12.8(\))]TJ -31.0845 -1.142 TD (200:1397)Tj /F8 1 Tf 3.5717 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(401.)-296.9(doi:)-300(10.1086/605889)]TJ -3.1587 -1.5712 TD [(40.)-500.6(Hannoun)-371.6(Z,)-371.5(Lin)-376.9(Z,)-371.5(Brackenridge)-373.1(S,)-375(Kuse)-366.6(N,)-371.3(Akahoshi)-372.2(T,)-374.8(Borthwick)-373.1(N,)-371.3(et)-367.3(al.)]TJ -0.9233 -1.142 TD [(Ide)-10.7(nti)]TJ /F7 1 Tf 2.3973 0 TD ()Tj /F6 1 Tf 0.5345 0 TD [(cat)-11.3(io)-9.4(n)-503.8(o)-10.2(f)-502.7(n)-9.7(ove)-11(l)-503.2(H)-9.4(IV-1)-16(-de)-14(riv)-11.4(ed)-513.8(HLA)-14.4(-E-)-9.7(bi)-9.4(ndi)-15.4(ng)-507.5(pe)-11.6(pt)-12.2(ide)-10.8(s.)]TJ /F15 1 Tf 24.4351 0 TD [(Im)-11.8(mun)-11.7(ol)-507.9(L)-11.6(ette)-15.3(rs)]TJ /F6 1 Tf 6.8519 0 TD (.)Tj -34.2188 -1.142 TD [(\(2018\))-297.6(202:65)]TJ /F8 1 Tf 5.5155 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(72.)-298.1(doi:)-308.1(10.1016/j.imlet.2018.08.005)]TJ -5.1025 -1.5712 TD [(41.)-500.6(Nattermann)-284.5(J,)-286.1(Nischalke)-285.4(HD,)-277.3(Hofmeister)-285.5(V,)-283.7(Kupfer)-284.5(B,)-284.3(Ahlenstiel)-283.8(G,)-280.8(Feldmann)]TJ -0.9233 -1.142 TD [(G,)-345.6(et)-351.1(al.)-358.5(HIV-1)-349.4(infection)-352.6(leads)-353.7(to)-356.2(increased)-356.2(HLA-E)-353.8(expression)-355(resulting)-356(in)-349.1(impaired)]TJ T* [(fu)-23.2(n)-9.7(c)-13.3(t)-13.9(i)-15.4(o)-10.3(n)-528.1(o)-10.2(f)-527(n)-9.7(a)-16.5(t)-13.9(u)-14.5(ra)-25.1(l)-519.4(k)-13.2(i)-15.4(l)-17.3(l)-9.2(e)-13.3(r)-526.9(c)-13.3(e)-13.3(l)-9.2(l)-17.3(s)0(.)]TJ /F15 1 Tf 13.89 0 TD [(A)-16.2(n)-11.6(t)-13.9(i)-13.5(vi)-21.9(r)-16.7(a)-13.1(l)-522.3(T)-14.4(he)-26.4(r)]TJ /F6 1 Tf 6.0986 0 TD 0.0151 Tc [(.)-512(\()1.7(200)15.1(4)-6.9(\))-508.5(10:)15.1(9)-8.7(5)]TJ /F8 1 Tf 6.9999 0 0 6.9999 487.7857 612.3967 Tm 0 Tc ()Tj /F6 1 Tf 0.5183 0 TD 0.0144 Tc [(107)14.4(.)-519.7(d)-0.1(oi:)-512.7(1)-0.7(0)14.4(.)-9.4(11)14.4(7)-7.6(7)-0.7(/)]TJ -26.6461 -1.1501 TD 0 Tc (135965350501000107)Tj 0.9233 -1.5712 TD [(42.)-500.6(Romagnani)-478.3(C,)-470.5(Pietra)-469.5(G,)-475.2(Falco)-468.8(M,)-476.4(Millo)-471.1(E,)-467.3(Mazzarino)-482.1(P,)-467.3(Biassoni)-473.4(R,)-472(et)-472.6(al.)]TJ -0.9233 -1.142 TD 0.0148 Tc (Identi)Tj /F7 1 Tf 2.4621 0 TD 0 Tc ()Tj /F6 1 Tf 0.5507 0 TD 0.0168 Tc [(cation)-511.3(of)-510.2(HLA)7.3(-)-1.8(E)3.8(-)-1.8(speci)]TJ /F7 1 Tf 9.7999 0 TD 0 Tc ()Tj /F6 1 Tf 0.5426 0 TD 0.0164 Tc [(c)-515.2(a)-0.1(llo)6.2(reactive)-515.2(T)-511.6(l)-0.9(y)4.7(m)-5.8(ph)4.8(ocytes:)-518.8(A)-511.5(cell)-511.1(subset)-515.8(that)]TJ -13.3555 -1.142 TD 0 Tc [(undergoes)-355.6(preferential)-355.5(expansion)-359(in)-357.2(mixed)-350.7(lymphocyte)-358.6(culture)-354.5(and)-356.7(displays)-358.3(a)-348.6(broad)]TJ T* [(cytolytic)-477.1(activity)-484.4(against)-489.3(allogeneic)-486.3(cells.)]TJ /F15 1 Tf 17.3321 0 TD [(Proc)-484.5(Natl)-484.1(Acad)-482.7(Sci)]TJ /F6 1 Tf 8.0586 0 TD [(.)-478.5(\(2002\))-483.8(99:11328)]TJ /F8 1 Tf 7.3621 0 TD ()Tj /F6 1 Tf 0.5102 0 TD (33.)Tj -33.2631 -1.142 TD [(doi:)-300(10.1073/pnas.172369799)]TJ 0.9233 -1.5712 TD [(43.)-500.6(Kunze-Schumacher)-243(H,)-236.9(Blasczyk)-231.7(R,)-237.1(Bade-Doeding)-243.3(C.)-227.5(Soluble)-238.5(HLA)-233.1(technology)-236.5(as)]TJ -0.9233 -1.142 TD 0.5274 Tc [(as)527.4(t)505(r)510.7(a)510.9(t)513.5(e)514.2(g)507.6(yt)513.5(oe)514.1(v)507.6(a)527.4(l)501.7(u)512.9(a)510.8(t)513.5(et)513.5(h)515.8(e)-4.2(i)527.4(m)506(p)512.9(a)510.9(c)514.1(t)-4.8(o)517.2(f)-7.7(H)518(L)507.7(Am)513.3(i)512(s)527.4(m)504.7(a)510.8(t)513.5(c)514.1(h)507.7(e)514.1(s)510.7(.)]TJ /F15 1 Tf 24.5323 0 TD 0.5303 Tc [(JI)530.3(m)502.3(m)517.6(u)518.6(n)518.7(o)512.4(l)-0.1(R)515.9(e)520.3(s)]TJ /F6 1 Tf 6.4793 0 TD 0.5352 Tc [(.\()521.8(2)520.1(0)535.2(1)513.2(4)520.1(\))]TJ -31.0116 -1.142 TD 0 Tc [(2014:246171.)-297.8(doi:)-300(10.1155/2014/246171)]TJ 0.9233 -1.5712 TD [(44.)-500.6(Michalsson)-284.4(J,)-269.9(Matos)-282(C,)-268(Achour)-278.4(A,)-277.5(Lanier)-277.7(LL,)-275.1(Krre)-280(K,)-274.2(Sderstrm)-282(K.)-274.2(A)-276.8(signal)]TJ -0.9233 -1.142 TD 0.0238 Tc [(pept)-6.3(ide)-524(d)-6.9(erived)-525.3(from)-524.9(hsp)-6.9(6)0.7(0)-525.8(b)5.5(ind)-6.9(s)-519.3(HLA-E)-523.8(a)-0.9(nd)-525.3(inte)-5.7(rfere)-5.7(s)-519.3(with)-530.5(CD94/NKG)-5.2(2A)]TJ T* 0 Tc (recognition.)Tj /F15 1 Tf 5.0782 0 TD [(J)-303.5(Exp)-299(Med)]TJ /F6 1 Tf 4.1792 0 TD [(.)-300.3(\(2002\))-305.7(196:1403)]TJ /F8 1 Tf 6.9977 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(14.)-306.2(doi:)-300(10.1084/jem.20020797)]TJ -15.8419 -1.5712 TD [(45.)-500.6(Terrazzano)-289.8(G,)-280.8(Sica)-289.9(M,)-282(Gianfrani)-283.6(C,)-284.2(Mazzarella)-284.3(G,)-288.9(Maurano)-284.7(F,)-282.5(Giulio)-288.2(BD,)-284.2(et)-278.2(al.)]TJ -0.9233 -1.1501 TD [(Gliadin)-409.5(regulates)-417.4(the)-411.3(NK-dendritic)-416.8(cell)-409.3(cross-talk)-420.2(by)-410.8(HLA-E)-410.5(surface)-415.5(stabilization.)]TJ /F15 1 Tf 34.1216 0 TD (J)Tj -34.1216 -1.142 TD (Immunol)Tj /F6 1 Tf 3.6608 0 TD [(.)-300.3(\(2007\))-305.7(179:372)]TJ /F8 1 Tf 6.5198 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(81.)-298.1(doi:)-308.1(10.4049/jimmunol.179.1.372)]TJ -9.7675 -1.5712 TD [(46.)-500.6(Bade-Doeding)-291.9(C,)-276.1(Cano)-287.7(P,)-281(Huyton)-288.3(T,)-277.6(Badrinath)-285.2(S,)-285.9(Eiz-Vesper)-288.1(B,)-284.3(Hiller)-283.7(O,)-282.2(et)-278.2(al.)]TJ -0.9233 -1.142 TD [(Mismatches)-339.9(outside)-341.5(exons)-343.3(2)-339(and)-340.5(3)-339(d)0(o)-332.6(not)-341.7(alter)-344.9(the)-338.5(peptide)-342.4(motif)-337.7(of)-342.9(the)-338.4(allele)-337.9(group)]TJ T* (B*44:02P.)Tj /F15 1 Tf 4.1954 0 TD [(Hum)-297.8(Immunol)]TJ /F6 1 Tf 6.0177 0 TD [(.)-300.4(\(2011\))-297.6(72:1039)]TJ /F8 1 Tf 6.5117 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(44.)-306.2(doi:)-300(10.1016/j.humimm.2011.08.004)]TJ -16.3116 -1.5712 TD [(47.)-500.6(Kraemer)-461.3(T,)-455.8(Celik)-464.1(AA,)-457.1(Huyton)-458.4(T,)-455.8(Kunze-Schumacher)-469.7(H,)-455.6(Blasczyk)-458.5(R,)-455.8(Bade-)]TJ -0.9233 -1.142 TD [(Dding)-361.3(C.)-365.2(HLA-E:)-362.6(presentation)-366.4(of)-367.2(a)-356.7(broader)-365.5(peptide)-366.7(repertoire)-370.6(impacts)-363.6(the)-362.8(cellular)]TJ T* 0.0407 Tc [(immune)-539.5(respo)6.2(n)-1.4(se)]TJ /F8 1 Tf 7.9534 0 TD 0 Tc ()Tj /F6 1 Tf 0.9557 0 TD 0.0407 Tc [(Implication)6.7(s)-542.9(on)-536(HSCT)-535.9(outcome.)]TJ /F15 1 Tf 15.4288 0 TD 0.0352 Tc [(St)-11.1(em)-544.5(C)-12(e)0.9(l)-9.3(l)-1.2(s)-547(I)-4.4(n)-8.8(t)]TJ /F6 1 Tf 6.4955 0 TD 0.0394 Tc [(.)-544.4(\()1.7(2015\))]TJ -30.8334 -1.142 TD 0 Tc [(2015:346714.)-297.8(doi:)-300(10.1155/2015/346714)]TJ 0.9233 -1.5712 TD 0.0297 Tc [(48)6.6(.)-505.5(P)0.5(u)7.1(r)-3.2(cell)-530.2(AW,)-529.8(R)3.9(ama)5.1(r)-3.2(a)5(t)-0.4(hinam)-527.1(S)3.6(H,)-529.8(Ternette)-526.2(N.)-521.7(Mas)4.9(s)-529.6(s)4.9(p)-1(ectr)4.9(om)7.5(etry)]TJ /F8 1 Tf 30.6228 0 TD 0 Tc ()Tj /F6 1 Tf 0.5345 0 TD 0.0337 Tc [(ba)9(se)12.3(d)]TJ -32.0807 -1.142 TD 0 Tc (identi)Tj /F7 1 Tf 2.3002 0 TD ()Tj /F6 1 Tf 0.5264 0 TD [(cation)-411.2(of)-399.6(MHC-bound)-409.2(peptides)-407.7(for)-408.2(immunopeptidomics.)]TJ /F15 1 Tf 23.9572 0 TD [(Nat)-407.2(Protoc)]TJ /F6 1 Tf 4.4302 0 TD [(.)-397.5(\(2019\))]TJ -31.2141 -1.1501 TD (14:1687)Tj /F8 1 Tf 3.0939 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(707.)-296.9(doi:)-300(10.1038/s41596-019-0133-y)]TJ -2.6808 -1.5712 TD [(49.)-500.6(Hogan)-316.9(MJ,)-316.3(Maheshwari)-314(N,)-314.6(Begg)-312.3(BE,)-313.6(Nicastri)-316.4(A,)-318(Hedgepeth)-318.1(EJ,)-315.4(Muramatsu)-315.8(H,)]TJ -0.9233 -1.142 TD [(et)-286.3(al.)-285.6(Cryptic)-288(MHC-E)-290(epitope)-289.5(from)-292.7(in)]TJ /F7 1 Tf 15.2507 0 TD ()Tj /F6 1 Tf 0.5345 0 TD [(uenza)-286.1(elicits)-291.4(a)-283.8(potent)-294.3(cytolytic)-290.8(T)-285(cell)-287.9(response.)]TJ /F15 1 Tf -15.7852 -1.142 TD [(Nat)-293.8(Immunol)]TJ /F6 1 Tf 5.4507 0 TD [(.)-300.3(\(2023\))-305.7(24:1933)]TJ /F8 1 Tf 6.5198 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(46.)-298.1(doi:)-300(10.1038/s41590-023-01644-5)]TJ -11.5575 -1.5712 TD [(50.)-500.6(Strong)-412.3(RK,)-413.4(Holmes)-413.1(MA,)-413(Li)-415.8(P,)-410.6(Braun)-414(L,)-417.3(Lee)-410.7(N,)-411.8(Geraghty)-416.2(DE.)-410.5(HLA-E)-410.5(allelic)]TJ -0.9233 -1.142 TD [(variants:)-377.1(Correlating)-376.4(dif)58.6(ferential)-383.9(expression,)-380(peptide)-374.8(af)]TJ /F7 1 Tf 22.1673 0 TD ()Tj /F6 1 Tf 0.5264 0 TD [(nities,)-384.7(crystal)-373.4(structures,)-385.6(and)]TJ -22.6938 -1.142 TD [(thermal)-297.8(stabilities*.)]TJ /F15 1 Tf 8.0424 0 TD [(J)-303.5(Biol)-297.6(Chem)]TJ /F6 1 Tf 4.7785 0 TD [(.)-300.3(\(2003\))-305.7(278:5082)]TJ /F8 1 Tf 6.9977 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(90.)-298.1(doi:)-300(10.1074/jbc.M208268200)]TJ -19.4055 -1.5712 TD [(51.)-500.6(Sylvester-Hvid)-364.2(C,)-357.1(Kristensen)-358.3(N,)-355.1(Blicher)-354.9(T,)-358.6(Ferre)]TJ /F19 1 Tf 21.4303 0.0405 TD ()Tj /F6 1 Tf 0.3969 -0.0405 TD [(H,)-350.3(Lauemller)-361.7(SL,)-354.3(Wolf)-356(XA,)]TJ -22.7505 -1.142 TD [(et)-343(al.)-350.4(Establishment)-352.5(of)-351(a)-348.6(quantitative)-349.5(ELISA)-350.9(capable)-352.8(of)-342.9(determining)-358.4(peptide)]TJ /F8 1 Tf 31.2789 0 TD ()Tj /F6 1 Tf 0.8585 0 TD (MHC)Tj -32.1374 -1.142 TD 0.0482 Tc [(cla)7.4(s)-0.9(s)-551.6(I)-542.1(inte)-5.5(r)7.2(a)-0.7(c)-5.5(t)1.9(io)5.6(n.)]TJ /F15 1 Tf 9.2249 0 TD 0.0472 Tc [(Tissue)-545.9(A)-1.4(nti)-6.8(g)1.5(ens)]TJ /F6 1 Tf 7.1029 0 TD 0.0476 Tc [(.)-552.4(\()1.8(2002\))-548.9(59:251)]TJ /F8 1 Tf 7.1515 0 TD 0 Tc ()Tj /F6 1 Tf 0.5588 0 TD 0.0476 Tc [(8.)-544.3(doi:)-552.4(10.)6.4(1)-7.9(0)0.2(34/j.1399-)]TJ -24.0382 -1.142 TD 0 Tc (0039.2002.590402.x)Tj 0.9233 -1.5712 TD [(52.)-500.6(Walters)-397.9(LC,)-384.9(Harlos)-394.6(K,)-387.6(Brackenridge)-397.4(S,)-391.2(Rozbesky)-397.9(D,)-389.4(Barrett)-390.7(JR,)-393(Jain)-391.9(V,)-389(et)-391.6(al.)]TJ -0.9233 -1.1501 TD 0.0188 Tc [(P)5.8(a)2.3(thog)7.1(en-de)5.6(r)-6(ived)-522.2(HLA-E)-512.6(bound)-514.1(epito)8.6(p)]TJ 17.1864 0 TD 0.0182 Tc [(es)-516.8(reveal)-517.4(br)-6.6(oad)-514.7(p)-4.5(rim)-4(a)1.6(ry)-520(anchor)-516.8(p)-4.5(o)-0.1(cket)]TJ -17.1864 -1.142 TD 0 Tc [(tolerability)-463.4(and)-462(conformationally)-470.9(malleable)-467.9(peptide)-472(binding.)]TJ /F15 1 Tf 25.4799 0 TD [(Nat)-463.9(Commun)]TJ /F6 1 Tf 5.6694 0 TD [(.)-462.3(\(2018\))]TJ -31.1493 -1.142 TD [(9:3137.)-295.3(doi:)-308.1(10.1038/s41467-018-05459-z)]TJ 0.9233 -1.5712 TD [(53.)-500.6(Joosten)-416.2(SA,)-425(Meijgaarden)-418.3(K,)-411.9(Weeren)-426.5(P,)-410.6(Kazi)-415.9(F,)-420.2(Geluk)-419.3(A,)-415.2(Savage)-419.9(NDL,)-415.3(et)-415.9(al.)]TJ -0.9233 -1.142 TD [(Mycobacterium)-298.8(tuberculosis)-294.2(Peptides)-300.9(Presented)-294.5(by)-297.4(HLA-E)-297.1(Molecules)-291.9(Are)-298.6(_targets)-292.8(for)]TJ T* [(Human)-323.6(CD8+)-325.9(T-Cells)-327.6(with)-319.6(Cytotoxic)-333.5(as)-324.8(well)-326.5(as)-324.8(Regulatory)-321.9(Activity.)]TJ /F15 1 Tf 28.1769 0 TD [(PloS)-324(Pathogens)]TJ /F6 1 Tf 6.042 0 TD (.)Tj -34.2188 -1.142 TD [(\(2010\))-297.6(6:e1000782.)-305.2(doi:)-300(10.1371/journal.ppat.1000782)]TJ 0.9233 -1.5712 TD 0.0368 Tc [(54)5.6(.)-498.4(S)2.7(a)4(l)3.3(er)3.9(n)2.8(o)2.3(-Gon)2.8(c)]TJ /F19 1 Tf 7.6294 0 TD 0 Tc ()Tj /F6 1 Tf 0.0567 0 TD 0.5677 Tc [(a)534.9(l)526.1(v)539.8(e)530.2(sR)533.8(,F)528.9(e)538.2(r)526.7(n)533.7(a)534.9(n)533.7(d)528.9(e)538.2(z)530.2(-)532.9(V)535.8(i)536.1()525.6(aM)537.5(,L)531.8(e)530.2(w)532(i)536.1(n)533.7(s)534.8(o)533.2(h)531.8(n)-0.9(D)535.4(M)529.4(,S)533.6(z)538.3(t)529.5(e)530.1(i)536.1(nM)537.5(B)527.1(.)]TJ 6.9999 0 0 6.9999 304.8944 213.3921 Tm 0.0144 Tc [(Ide)-7(n)-3.4(ti)]TJ /F7 1 Tf 2.4702 0 TD 0 Tc ()Tj /F6 1 Tf 0.5507 0 TD 0.0167 Tc [(ca)-8(tion)-519.5(of)-518.4(a)-510.1(h)-3(u)-5.9(m)2.6(an)-519.5(HL)5.1(A-E-rest)-5.3(ricted)-524.3(CD8+)-520.9(T)-519.4(c)3.4(ell)-510.8(s)0(u)-5.9(b)-1.6(set)-523.6(i)1.3(n)-519.5(v)5(olunt)-5.3(e)3.4(ers)]TJ -3.021 -1.142 TD 0 Tc [(immunized)-472.7(with)-473.5(salmonella)-473(enterica)-476.5(serovar)-474.4(typhi)-472.2(strain)-477.7(ty21a)-469.2(typhoid)-472.7(vaccine.)]TJ /F15 1 Tf 34.1216 0 TD (J)Tj -34.1216 -1.142 TD (Immunol)Tj /F6 1 Tf 3.6608 0 TD [(.)-300.3(\(2004\))-305.7(173:5852)]TJ /F8 1 Tf 6.9977 0 TD ()Tj /F6 1 Tf 0.5102 0 TD [(62.)-298.1(doi:)-308.1(10.4049/jimmunol.173.9.5852)]TJ -10.2454 -1.8628 TD [(55.)-500.6(Lampen)-493(MH,)-485.8(Hassan)-490.6(C,)-494.8(Sluijter)-490.7(M,)-484.5(Geluk)-492.2(A,)-488.1(Dijkman)-491.2(K,)-484.8(Tjon)-492.4(JM,)-486.4(et)-488.8(al.)]TJ -0.9233 -1.142 TD [(Alternative)-467.1(peptide)-463.9(repertoire)-467.8(of)-464.4(HLA-E)-459.1(reveals)-469.9(a)-462(binding)-467.5(motif)-459.2(that)-469(is)-461.3(strikingly)]TJ T* 0.0912 Tc [(si)-5.2(m)-3.9(i)-5.2(l)-7.1(a)1.8(r)-597.7(t)-3.7(o)-599.4(H)-7.3(LA)-7.4(-A)-7.4(2)-4.8(.)]TJ /F15 1 Tf 10.5694 0 TD 0.0935 Tc [(Mol)-598.9(I)-2.8(mmun)-7.2(o)2.7(l)]TJ /F6 1 Tf 6.7952 0 TD 0.096 Tc [(.)-593.1(\()1.6(201)8.1(3\))-597.7(53:)6.2(126)]TJ /F8 1 Tf 7.8562 0 TD 0 Tc ()Tj /F6 1 Tf 0.6074 0 TD 0.0956 Tc [(31)7.7(.)-601.6(d)0.1(oi:)-593.5(10.)5.8(1016/)]TJ -25.8281 -1.142 TD 0 Tc (j.molimm.2012.07.009)Tj 0.9233 -1.8547 TD [(56.)-500.6(McMurtrey)-225.3(C,)-219.4(Harrif)54.3(f)-219.2(MJ,)-219.2(Swarbrick)-228.6(GM,)-221.9(Duncan)-225.8(A,)-220.8(Cansler)-219.8(M,)-225.3(Null)-225.3(M,)-217.2(et)-221.5(al.)-220.8(T)]TJ -0.9233 -1.1501 TD [(cell)-296(recognition)-301.6(of)-302.4(Mycobacterium)-298.8(tuberculosis)-302.3(peptides)-302.4(presented)-304.1(by)-297.4(HLA-E)-305.2(derived)]TJ 0 -1.142 TD 0.0545 Tc [(from)-550.9(infected)-559.4(hu)-8.6(man)-562.7(cells.)]TJ /F15 1 Tf 13.3798 0 TD 0.0569 Tc [(PloS)-546.1(One)]TJ /F6 1 Tf 4.2763 0 TD 0.0554 Tc [(.)-560.8(\()1.5(2017\))-557.3(12:)6.1(e)-6.5(0)-0.1(1)8(88288.)-560.8(doi:)-552.7(10.1371/)]TJ -17.6561 -1.142 TD 0 Tc (journal.pone.0188288)Tj 0.9233 -2.0005 TD [(57.)-500.6(Mart)]TJ /F20 1 Tf 3.6689 0 TD ()Tj /F19 1 Tf 0.2916 0.0405 TD ()Tj /F6 1 Tf -0.0324 -0.0405 TD [(n)-325.6(Almaza)]TJ /F19 1 Tf 3.8552 0.0405 TD ()Tj /F6 1 Tf 0.0567 -0.0405 TD [(n)-325.6(N)0(,)-322.7(Sala)-327.5(BM,)-322.6(Sandalova)-333.7(T,)-326.2(Sun)-325.7(Y,)-323.3(Resink)-328.9(T,)-326.2(Cichocki)-327.3(F,)-323(et)-326.8(al.)]TJ -8.7633 -1.142 TD [(Non-classical)-276.8(HLA-E)-280.9(restricted)-277.1(CMV)-280.9(15-mer)-279(peptides)-278.1(are)-281.4(recognized)-285.3(by)-273.1(adaptive)-278.5(NK)]TJ T* 0.016 Tc [(ce)-5.4(lls)-527.1(and)-525(i)0.6(nd)-6.6(u)-6.6(c)2.8(e)-523.7(m)-6.2(em)-6.2(ory)-522.2(r)-8.8(es)-8.8(pons)-8.8(e)-5.4(s)-0.7(.)]TJ /F15 1 Tf 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startxref 116 %%EOF %BeginExifToolUpdate 1187 0 obj << /Type /Metadata /Subtype /XML /Length 4932 >> stream Maya Weitzen Mohammad Shahbazy Saketh Kapoor Etienne Caron Weitzen M, Shahbazy M, Kapoor S and Caron E (2024) Deciphering the HLA-E immunopeptidome with mass spectrometry: an opportunity for universal mRNA vaccines and T-cell-directed immunotherapies. Front. Immunol. 15:1442783. doi: 10.3389/fimmu.2024.1442783 Copyright © 2024 Weitzen, Shahbazy, Kapoor and Caron. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. HLA-E peptides immunopeptidome mass spectrometry vaccine design viral infection cancer immunotherapy Deciphering the HLA-E immunopeptidome with mass spectrometry: an opportunity for universal mRNA vaccines and T-cell-directed immunotherapies HLA-E peptides; immunopeptidome; mass spectrometry; vaccine design; viral infection; cancer immunotherapy True https://www.frontiersin.org/legal/copyright-statement endstream endobj 446 0 obj << /PageMode /UseOutlines /Names 449 0 R /Outlines 1173 0 R /Pages 437 0 R /OpenAction [ null /Fit ] /Threads 447 0 R /Type /Catalog /PageLabels 435 0 R /Metadata 1187 0 R >> endobj xref 0 1 0000000443 65535 f 443 2 0000000444 00001 f 0000000000 00001 f 446 1 0004321250 00000 n 1187 1 0004316223 00000 n trailer << /Size 1188 /Prev 116 /Root 446 0 R /ID [ <3694EF1210FD14AFD48030CD1A8092D3> <7c2558B235E7774DADB3AB0D9024EEF3> ] >> %EndExifToolUpdate 4316201 startxref 4321455 %%EOF
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