Mechanism of chloride secretion induced by carbachol in a colonic epithelial cell line.
K Dharmsathaphorn, SJ Pandol - The Journal of clinical …, 1986 - Am Soc Clin Investig
Serosal application of carbachol to T84 cell monolayers mounted in an Ussing chamber
caused an immediate increase in short circuit current (Isc) that peaked within 5 min and
declined rapidly thereafter, although a small increase in Isc persisted for approximately 30
min. The increase in Isc was detectable with 1 microM carbachol; half-maximal with 10
microM carbachol; and maximal with 100 microM carbachol. Unidirectional Na+ and Cl-flux
measurements indicated that the increase in Isc was due to net Cl-secretion. Carbachol did …
caused an immediate increase in short circuit current (Isc) that peaked within 5 min and
declined rapidly thereafter, although a small increase in Isc persisted for approximately 30
min. The increase in Isc was detectable with 1 microM carbachol; half-maximal with 10
microM carbachol; and maximal with 100 microM carbachol. Unidirectional Na+ and Cl-flux
measurements indicated that the increase in Isc was due to net Cl-secretion. Carbachol did …