Molecularly Imprinted Polymer Based Biosensors for Environmental, Agricultural and Food Safety Applications

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: 30 April 2025 | Viewed by 1615

Special Issue Editors


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Guest Editor
School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
Interests: biosensors; nucleic acid nanotechnology; molecular imprinting technology; food safety and rapid detection

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Guest Editor
School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, China
Interests: biosensors; food safety; rapid detection

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Guest Editor
School of Chemistry and Chemical Engineering, Lingnan Normal University, Zhanjiang 524048, China
Interests: optical sensors; nanomaterials; food safety; biosensors

Special Issue Information

Dear Colleagues,

Molecular imprinted polymers (MIPs) as artificial receptors to mimic the specific binding manner of antibodies and enzymes have attracted much attention. The features of MIPs, including predicable recognition cavity, excellent chemical stability, simple synthesis, and low cost, enabled their high-performance recognition of various kinds of _targets, ranging from metal ions, small molecules, biomacromolecules, and even entire cells. To implement the quantitative analysis of these biomarkers is of great importance in the fields of Environment, Agriculture, and Food Safety. By endowing MIPs with unique optical and electrical characteristics, the binding event can be transduced into a detectable output signal. As-prepared MIP-based biosensors with highly sensitive, rapid, and selective sensing performance have become a hot topic in recent years.

In this regard, this Special Issue aims to gather both original research papers as well as reviews on the aspect of MIP-based biosensors. This includes the design and synthesis of functional MIPs, environmental analysis, and food rapid detection. Theoretical calculation and computer simulation related to the binding mechanism of MIPs is also encouraged. The development of lab-on-a-chip devices, wearable and portable/handheld MIP-based biosensors for rapid analysis in non-laboratory settings, and MIP-based bioimaging systems are of special interest. Reviews should provide an in-depth analysis of the most recent research in a specific context or discuss the current and future issues related to MIPs in the biosensing field.

Dr. Tai Ye
Dr. Qianjin Li
Dr. Guohua Zhou
Guest Editors

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Keywords

  • molecular imprinted polymers
  • optical sensors
  • nanomaterials
  • food safety
  • environmental pollution
  • biomimetic assay
  • catalytic receptor
  • electropolymerized molecularly imprinted polymer
  • paper-based analytical devices

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Published Papers (1 paper)

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Research

12 pages, 3242 KiB  
Article
Electrochemical Impedance Spectroscopy-Based Microfluidic Biosensor Using Cell-Imprinted Polymers for Bacteria Detection
by Shiva Akhtarian, Satinder Kaur Brar and Pouya Rezai
Biosensors 2024, 14(9), 445; https://doi.org/10.3390/bios14090445 - 18 Sep 2024
Viewed by 1168
Abstract
The rapid and sensitive detection of bacterial contaminants using low-cost and portable point-of-need (PoN) biosensors has gained significant interest in water quality monitoring. Cell-imprinted polymers (CIPs) are emerging as effective and inexpensive materials for bacterial detection as they provide specific binding sites designed [...] Read more.
The rapid and sensitive detection of bacterial contaminants using low-cost and portable point-of-need (PoN) biosensors has gained significant interest in water quality monitoring. Cell-imprinted polymers (CIPs) are emerging as effective and inexpensive materials for bacterial detection as they provide specific binding sites designed to capture whole bacterial cells, especially when integrated into PoN microfluidic devices. However, improving the sensitivity and detection limits of these sensors remains challenging. In this study, we integrated CIP-functionalized stainless steel microwires (CIP-MWs) into a microfluidic device for the impedimetric detection of E. coli bacteria. The sensor featured two parallel microchannels with three-electrode configurations that allowed simultaneous control and electrochemical impedance spectroscopy (EIS) measurements. A CIP-MW and a non-imprinted polymer (NIP)-MW suspended perpendicular to the microchannels served as the working electrodes in the test and control channels, respectively. Electrochemical spectra were fitted with equivalent electrical circuits, and the charge transfer resistances of both cells were measured before and after incubation with _target bacteria. The charge transfer resistance of the CIP-MWs after 30 min of incubation with bacteria was increased. By normalizing the change in charge transfer resistance and analyzing the dose–response curve for bacterial concentrations ranging from 0 to 107 CFU/mL, we determined the limits of detection and quantification as 2 × 102 CFU/mL and 1.4 × 104 CFU/mL, respectively. The sensor demonstrated a dynamic range of 102 to 107 CFU/mL, where bacterial counts were statistically distinguishable. The proposed sensor offers a sensitive, cost-effective, durable, and rapid solution for on-site identification of waterborne pathogens. Full article
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