Nutrients doi: 10.3390/nu17010086

Authors: Jingjing Liu Xinye Qi Rizhen Wang Junling Zhang Shaoke Lu Guangliang Xie Yinghua Qin Dongqing Ye Qunhong Wu

Background: There are few studies examining the physical developmental phenotypes of nutritional deficiency diseases (NDDs) among Chinese children aged 1–7 years by anthropometrics and clarifying the specific NDD categories that caused growth faltering. Methods: A total of 3054 cases of NDDs in children aged 1–7 years were investigated. The age, height, and weight of children with NDDs were adjusted by using the skewness coefficient–median–coefficient of variation method, and the results were compared with the WHO standardized level. Comparisons of specific categories of NDDs were performed with respect to the age-specific height standardized values (HAZ), the age-specific weight standardized values (WAZ), and the age-specific body mass index standard values (BAZ). Result: The subtypes of NDDs among Chinese children were mainly characterized by single and co-morbid deficiencies. Calcium deficiency, vitamin A deficiency, vitamin D deficiency, and vitamin B deficiency were the main types, accounting for 11.33%, 9.26%, 8.70%, and 6.29% of the total confirmed cases, respectively; protein–energy malnutrition combined with vitamin C deficiency was the most common type of combined nutrient deficiency (5.76%). The HAZ (−0.0002), WAZ (−0.0210), and BAZ (−0.0018) of children aged 1–3 years with NDDs was lower than the WHO standard (0), as were the HAZ (−0.0003), WAZ (−0.0219), and BAZ (−0.0019) of children aged 3–7 years. The anthropometrics of children with NDDs aged 1–7 years showed that the HAZ and BAZ were slightly lower than the WHO average level, whereas the WAZ was significantly different from the WHO average. The co-morbidity of vitamin A deficiency and vitamin D deficiency, calcium deficiency and vitamin C deficiency, vitamin D deficiency and vitamin C deficiency, and iodine deficiency and vitamin C deficiency were associated with the WAZ. Interpretation: The specific categories of NDDs consist mainly of calcium deficiencies, vitamin A deficiencies, and vitamin D deficiencies. The main signs of growth retardation were low weight and height, which were driven by the specific single and co-morbid micronutrient deficiencies.

Nutrients doi: 10.3390/nu17010085

Authors: Aurelio Seidita Federica Latteri Mirco Pistone Alessandra Giuliano Luca Bertoncello Giorgia Cavallo Marta Chiavetta Francesco Faraci Alessia Nigro Alessandro Termini Laura Verona Agnese Ammannato Salvatore Accomando Francesca Cavataio Maria Letizia Lospalluti Michele Citrano Diana Di Liberto Maurizio Soresi Pasquale Mansueto Antonio Carroccio

Background/Objectives: An increasing number of studies have reported liver involvement in both children and adults with celiac disease (CD). This often manifests as isolated hypertransaminasemia or hepatic steatosis (HS). The aim of this study was to define the prevalence of hypertransaminasemia and HS in a pediatric population with CD before starting a gluten-free diet (GFD) and to analyze how the introduction of a GFD could modify this condition. We also conducted a state-of-the-art literature review of the association between hypertransaminasemia, metabolic dysfunction-associated steatotic liver disease (MASLD) and CD. Methods: We retrospectively reviewed the clinical charts of pediatric CD patients diagnosed in three different pediatric units of Sicily, analyzing clinical, laboratory, ultrasound, and histology data before and 12 months after the introduction of a GFD. Results: A total of 160 patients (65.0% females, median age 6.4 (0.8–13.2) years) were included; hypertransaminasemia and HS prevalences at diagnosis were 8.1% and 6.1%, respectively. Subjects with hypertransaminasemia were younger (p = 0.01) than those without and had higher frequencies of HS (p = 0.034) and anti-tissue transglutaminase (tTg) immunoglobulin (Ig)G positivity (p = 0.046). Subjects with HS were younger (p = 0.0001) and had a higher frequency of hypertransaminasemia (p = 0.029) compared to non-steatotic ones. After 12 months of a GFD, hypertransaminasemia and HS persisted in 53.8% and 50.0% of patients, respectively. Conclusions: The prevalences of hypertransaminasemia and HS in Sicilian pediatric CD patients seem to be lower than those reported in other geographical areas. A GFD can reverse the trend of liver involvement, although periods of longer than 12 months may be necessary. However, a GFD has been associated with an increased prevalence of HS, and so regular follow-up involving a nutritionist should be recommended to guide physicians in patient management.

Nutrients doi: 10.3390/nu17010084

Authors: Ana Vega-Rojas Carmen Haro Helena Molina-Abril Silvia Guil-Luna Jose Antonio Santos-Marcos Francisco Miguel Gutierrez-Mariscal Helena Garcia-Fernandez Javier Caballero-Villarraso Antonio Rodriguez-Ariza Jose Lopez-Miranda Pablo Perez-Martinez Antonio Hervas Antonio Camargo

Background/Objectives: Gut microbiota interacts with nutrients, which may be relevant to assigning a microbial signature to colorectal cancer (CRC). We aim to evaluate the potential of gut microbiota combined with dietary habits in the early detection of pathological findings related to CRC in the course of a screening program. Methodology: The colonoscopy performed on 152 subjects positive for fecal occult blood test showed that 6 subjects had adenocarcinoma, 123 had polyps, and 23 subjects had no pathological findings. Gut microbiota was analyzed by 16S metagenomic. Caret package was used to build the classification models in R. Results: Random forest (RF) classifier models were used to test the potential of gut microbiota alone or combined with dietary habits as a biomarker to discern between individuals with CRC-related lesions (polyps or adenocarcinoma) versus individuals without pathological findings. RF classifier models yielded an area under the curve of 0.790 using gut microbiota data, 0.710 using dietary habits data, and 0.804 in the combined model including gut microbiota and dietary habits data. The abundance of Suterella, Oscillospirales, Proteobacteria, and Burkholderiales was highly discriminant between groups, together with the consumption of fruit and vegetables and the consumption of carbonated and/or sweetened beverages. Conclusions: Our results suggest that the interaction between gut microbiota and dietary habits is relevant when a microbial signature is used as a marker in CRC. Moreover, gut microbiota signature and information about the dietary habits of the individuals seem to be important for improving screening programs for the early detection of CRC.

Nutrients doi: 10.3390/nu17010083

Authors: R. Taylor Pickering Ioanna Yiannakou Laura Lara-Castor M. Loring Bradlee Martha R. Singer Lynn L. Moore

Objectives: Given the considerable discrepancy in the literature regarding dietary protein and glucose homeostasis, we examined the prospective association between protein intake (total, animal, plant) and risk of type 2 diabetes mellitus or impaired fasting glucose (IFG). We also examined whether these associations differed by sex, body weight, or other risk factors. Methods: We included 1423 subjects, aged ≥ 30 years, in the Framingham Offspring Study cohort. Three-day dietary records at exams 3 and 5 were used to average protein intake and then adjusted for body weight residuals. Cox proportional hazard models were used to estimate hazard ratios (HR), adjusting for anthropometric, demographic, and lifestyle factors over ~16 years of follow-up. Results: Subjects with the highest total protein intakes (≥100 g men; ≥85 g women) had a 31% lower risk of type 2 diabetes/IFG (95% CI: 0.54, 0.87). The highest (vs. lowest) category of intake of animal protein was associated with a 32% lower risk of diabetes/IFG (95% CI: 0.55, 0.83), whereas plant protein was not. Beneficial trends of total protein, especially animal, were stronger for women (HR: 0.61; 95% CI: 0.42, 0.87) than for men (HR: 0.82; 95% CI 0.58, 1.15). Subjects with lower BMI who consumed more protein had the lowest risks of diabetes/IFG. Conclusions: Overall, in this prospective study, higher intake of total dietary protein, including the consumption of animal protein, particularly among individuals with lower BMI and higher physical activity levels, was inversely associated with risk of incident type 2 diabetes and IFG.

Nutrients doi: 10.3390/nu17010082

Authors: Karen E. Christensen Marie-Lou Faquette Daniel Leclerc Vafa Keser Yan Luan Jeanna L. Bennett-Firmin Olga V. Malysheva Alaina M. Reagan Gareth R. Howell Marie A. Caudill Teodoro Bottiglieri Rima Rozen

Background/Objectives: The MTHFR677C>T gene variant results in a thermolabile MTHFR enzyme associated with elevated plasma homocysteine in TT individuals. Health risks associated with the TT genotype may be modified by dietary and supplemental folate intake. Supplementation with methyltetrahydrofolate (methylTHF) may be preferable to folic acid because it is the MTHFR product, and does not require reduction by DHFR to enter one-carbon folate metabolism. In the Mthfr677C>T mouse model for this variant, female 677TT (TT) mice have an increased incidence of hepatic steatosis. The objective of this study was to compare the effects of methylTHF and folic acid supplementation on hepatic steatosis and one-carbon metabolism in this model. Methods: Male and female C57BL/6J 677CC (CC) and TT mice were fed control (CD), 5xmethylTHF-supplemented (MFSD), or 5xfolic-acid-supplemented (FASD) diets for 4 months. Liver sections were assessed for steatosis by Oil Red O staining. One-carbon metabolites were measured in the liver and plasma. MTHFR protein expression was evaluated in the liver. Results: MFSD had no significant effect on plasma homocysteine, liver SAM/SAH ratios, or hepatic steatosis in males or females as compared to CD. MTHFR protein increased in MFSD TT female liver, but remained <50% of the CC. FASD had no effect on plasma homocysteine but it decreased the liver MTHFR protein and SAM/SAH ratios, and increased hepatic steatosis in CC females. Conclusions; MethylTHF and folic acid supplementation had limited benefits for TT mice, while folic acid supplementation had negative effects on CC females. Further investigation is required to determine if these effects are relevant in humans.

Nutrients doi: 10.3390/nu17010080

Authors: Marta Pelczyńska Małgorzata Moszak Julita Wojciechowska Anita Płócienniczak Jan Potocki Joanna Blok Julia Balcerzak Mikołaj Zblewski Paweł Bogdański

The chronotype, the personal predisposition towards morning or evening activities, significantly influences health conditions, sleep, and eating regulations. Individuals with evening chronotypes are often at a higher risk for weight gain due to misalignment between their natural tendencies of functioning and social schedules, resulting in insufficient sleep, disruptions in eating habits, and decreased physical activity levels. Often, impaired glucose tolerance and changes in melatonin, adiponectin, and leptin secretion, along with alterations in the clock gene functions in subjects with evening preferences, may be predisposed to obesity. These disturbances contribute to metabolic dysregulation, which may lead to the subsequent onset of obesity complications, such as hypertension, type 2 diabetes, sleep apnea, and liver diseases. _targeting critical components of the circadian system and synchronizing people’s chronotypes with lifestyle conditions could deliver potential strategies for preventing and treating metabolic disorders. Thus, it is recommended to take a personalized chronobiological approach to maintain a normal body weight and metabolic health. Nevertheless, future studies are needed to identify the clear mechanisms between the chronotype and human health. This article provides a narrative review and discussion of recent data to summarize studies on the circadian rhythm in the context of obesity. The manuscript represents a comprehensive overview conducted between August and November 2024 using the National Library of Medicine browser (Medline, Pub-Med, Web of Science).

Nutrients doi: 10.3390/nu17010081

Authors: David J. Barry Sam S. X. Wu Matthew B. Cooke

Background: Recent evidence suggests that sarcopenia and subsequent changes in muscle mass and functional outcomes are linked to disruption to the gastrointestinal microbiota composition and/or function via the microbiota-gut-muscle axis. Despite growing interest, few studies have systemically analysed (1) the relationship between the gut microbiota, muscle mass and physical performance and (2) the effects of gut-modulating dietary interventions on these outcomes within older individuals with or without sarcopenia. Methods: Four electronic databases (PubMed, MEDLINE, Embase and Scopus) were searched for articles published from the year 2004 until July 2023. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) were followed. Revised Cochrane Risk of Bias (RoB 2.0) and Joanna Briggs Institute (JBI) critical appraisal checklist were utilised to evaluate the risk of bias within intervention and observational studies, respectively. Results: A total of 20 studies (14 observational and 6 interventional) involving 4071 older participants (mean age 69.9 years, 51.6% female) were included. There was significant heterogeneity regarding interventions and outcome measures used in these studies. Correlations between microbiota diversity and composition and sarcopenia-related functional outcomes were observed. Interventional studies _targeting the gut microbiota resulted in improved muscle strength, body composition or physical function in some, but not all, studies. Conclusions: Despite limitations in the studies reviewed, the findings provide further evidence that the development of sarcopenia is likely influenced by an altered gut microbial environment and that interventions _targeting the microbiome could hold therapeutic potential for the treatment or management of sarcopenia.

Nutrients doi: 10.3390/nu17010079

Authors: Dawn M. Emerson Melani R. Kelly Toni M. Torres-McGehee Nancy A. Uriegas Matthew O. Smith Allison B. Smith Kevin A. Kloesel

Exercise-associated hyponatremia (EAH) is commonly observed in endurance athletes, where prolonged physical exertion combined with being unaware of personal hydration needs can lead to excessive water consumption or inadequate sodium intake. Marching band (MB) is an emerging setting for sports medicine professionals. However, there is little research on non-musculoskeletal illnesses among these performing artists. This study is part of a larger cross-sectional study and seeks to examine whether MB artists have previously experienced EAH and the presence of known EAH risk factors (e.g., pre-existing medical conditions, medication use, nutritional behaviors). Active collegiate MB members (n = 1207; mean age = 19.6 ± 1.3 years) completed an online survey that characterized demographics, medical history, and nutrition behaviors. Statistical analyses included descriptives (mean, standard deviation) and frequencies for all data. Binomial logistic regressions analyzed the relationship and determined the odds ratio (OR) between previous EAH and EAH risk factors. Participants were from 23 different states, predominately White (82.9%), female (56.7%), and at NCAA Division I institutions (92%). A total of 74 (6.1%) participants reported previously experiencing EAH, 32 (43.2%) within the past year. Previous EAH occurrence was greatest among individuals who had kidney conditions (χ2(1) = 5.920, R2 = 0.013, OR = 15.708, p = 0.015); mood/neurological conditions (χ2(1) = 7.508, R2 = 0.017, OR = 2.154, p = 0.006), particularly anxiety (χ2(1) = 7.651, R2 = 0.017, OR = 3.590, p = 0.006); used mental health medications (χ2(1) = 7.512, R2 = 0.017, OR = 2.220, p = 0.006), particularly selective serotonin reuptake inhibitors (χ2(1) = 13.502, R2 = 0.030, OR = 3.297, p < 0.001); and who dieted (χ2(1) = 6.121, R2 = 0.014, OR = 1.913, p = 0.013) or perceived they had an eating disorder (χ2(1) = 9.502, R2 = 0.021, OR = 2.729, p = 0.002). Healthcare providers should be aware that MB artists may have pre-existing medication conditions, use medication, and have inadequate nutrition. _targeted hydration and nutritional education for MB artists is essential for effective prevention and early EAH recognition, improving health and performance for MB artists within demanding environments.

Nutrients doi: 10.3390/nu17010078

Authors: Natalia Cichon Weronika Grabowska Leslaw Gorniak Maksymilian Stela Piotr Harmata Michal Ceremuga Michal Bijak

Flavonoids are naturally occurring polyphenolic compounds known for their extensive range of biological activities. This review focuses on the inhibitory effects of flavonoids on acetylcholinesterase (AChE) and their potential as therapeutic agents for cognitive dysfunction. AChE, a serine hydrolase that plays a crucial role in cholinergic neurotransmission, is a key _target in the treatment of cognitive impairments due to its function in acetylcholine hydrolysis. Natural polyphenolic compounds, particularly flavonoids, have demonstrated significant inhibition of AChE, positioning them as promising alternatives or adjuncts in neuropharmacology. This study specifically examines flavonoids such as quercetin, apigenin, kaempferol, and naringenin, investigating their inhibitory efficacy, binding mechanisms, and additional neuroprotective properties, including their antioxidant and anti-inflammatory effects. In vitro, in vivo, and in silico analyses reveal that these flavonoids effectively interact with both the active and peripheral anionic sites of AChE, resulting in increased acetylcholine levels and the stabilization of cholinergic signaling. Their mechanisms of action extend beyond mere enzymatic inhibition, as they also exhibit antioxidant and anti-amyloidogenic properties, thereby offering a multifaceted approach to neuroprotection. Given these findings, flavonoids hold considerable therapeutic potential as modulators of AChE, with implications for enhancing cognitive function and treating neurodegenerative diseases. Future studies should prioritize the enhancement of flavonoid bioavailability, evaluate their efficacy in clinical settings, and explore their potential synergistic effects when combined with established therapies to fully harness their potential as neurotherapeutic agents.

Nutrients doi: 10.3390/nu17010076

Authors: Georgia Kakafoni Evangelia Zvintzou Smaro Kyroglou Katerina Giannatou Victoria Mparnia Patroklos Vareltzis Kyriakos E. Kypreos

Background/Objectives: Lavender has been utilized for its medicinal properties since ancient times, with numerous health benefits reported. This study aimed to valorize solid waste from lavender essential oil production by developing a novel lavender extract from solid lavender residues. The extract’s preclinical safety and efficacy were evaluated with emphasis on plasma lipid and lipoprotein metabolism, glucose tolerance, and adipose tissue metabolic activity. Methods: Male C57BL/6 mice were divided into four groups of five mice each and fed for 30 days with lavender extract encapsulated in 10% maltodextrin, mixed with a standard chow diet. The first group (Lav 1×) received 21.1 mg/kg/day, the second group (Lav 10×) received 211 mg/kg/day, and the third group (Lav 100×) received 2110 mg/kg/day. A placebo group consumed the standard diet without lavender extract. Key outcomes included plasma lipid and lipoprotein profiles, transaminase levels, HDL antioxidant and anti-inflammatory potential, glucose tolerance, and mitochondrial activity in white (WAT) and brown (BAT) adipose tissues. Results: The novel lavender extract induced dose-dependent improvements in lipid and lipoprotein metabolism, glucose tolerance, and adipose tissue activity. The 2110 mg/kg dose (100×) demonstrated the most significant beneficial effects, although it was associated with a slight elevation in hepatic transaminase levels, indicating potential mild hepatic stress. Conclusions: Overall, the novel lavender extract exhibits promising health benefits with no major safety concerns at the tested doses, supporting its potential for therapeutic applications.

Nutrients doi: 10.3390/nu17010077

Authors: Lorenza Francesca De Lellis Hammad Ullah Maria Vittoria Morone Daniele Giuseppe Buccato Alessandra Baldi Alessandro Di Minno Danaé S. Larsen Roberto Sacchi Maria Daglia

Background/Objectives: Malva sylvestris L. is rich in mucilage and is traditionally used for the management of numerous ailments including gastrointestinal disorders. Functional constipation (FC) is a gastrointestinal condition characterized by defecation anomalies such as infrequent stools, difficulty in stool passage, or both in the absence of pathological abnormalities. FC can be reduced through lifestyle factors and dietary intervention. This consumer-based survey aimed to assess the efficacy of a M. sylvestris extract-based food supplement on the improvement of FC. Methods: Healthy participants (n = 56), enrolled in a consumer-based survey, took a food supplement containing a chemically characterized M. sylvestris extract at a dose of 20 mL/day (containing 750 mg of M. sylvestris extract rich in food fiber and polyphenols) for 20 days on the advice of their pharmacist. The study evaluated bowel movement frequency (intestinal diary), stool consistency (Bristol Stool Form Scale, BSFS), and abdominal pain (Visual Analogue Scale, VAS), at baseline (T0), after 10 days (T1), and after 20 days (T2). Results: A significant increase in bowel movement frequency and stool consistency (p < 0.001) with a significant decrease in abdominal pain (p < 0.001) was observed. Additionally, this food supplement was well-tolerated as no adverse effects were reported by the enrolled subjects. Conclusion: M. sylvestris-based food supplement showed promising effectiveness and satisfaction in improving FC in healthy subjects, however, randomized clinical studies are needed to confirm these preliminary results.

Nutrients doi: 10.3390/nu17010075

Authors: Vibha Singhal Clarissa C. Pedreira Shubhangi Tuli Lea Abou Haidar Ana Lopez Lopez Meghan Lauze Hang Lee Miriam A. Bredella Madhusmita Misra

Background/Objective: Weight loss is associated with reductions in resting energy expenditure (REE), which are impacted by changes in body composition following sleeve gastrectomy (SG). Current data regarding changes in measured REE (mREE) and metabolic adaptation in adolescents after SG are limited. We evaluated changes in mREE, metabolic adaptation, and body composition in youths after SG vs. non-surgical (NS) controls over two years. Methods: Youths 14–22 years old undergoing SG (n = 24) and NS controls with severe obesity (n = 28) were recruited. mREE was determined using indirect calorimetry. Predicted REE (pREE) was calculated using regression equation derived from baseline data of our cohort and used to calculate pREE at follow up. Metabolic adaptation was calculated as mREE − pREE. We normalized REE to fat-free mass (FFM) and total body weight (TBW). Dual energy X-ray absorptiometry was used to measure body composition. Measurements were performed at baseline and two-years. Results: Baseline age, sex, and BMI were similar between groups. Greater decreases in BMI in SG vs. NS (−12.4 (−14.4, −9.8) vs. 2.2 (0.3, 3.5) kg/m2, p < 0.0001) and within-group decreases in mREE (401.0 ± 69.5 kcal/d; p < 0.0001) in SG were seen. mREE/FFM decreased within the SG group (p = 0.006), the two-year change in mREE/FFM and mREE/TBW did not differ between groups (p = 0.14 and 0.24). There was no metabolic adaptation within SG. Conclusions: Despite significant decreases in BMI after SG in youths, no metabolic adaptation was present at two years. This implies that by two years, metabolism has reached a steady state and weight changes after this should be addressed in an unbiased way.

Nutrients doi: 10.3390/nu17010074

Authors: Anna Deskur Filip Ambrożkiewicz Emilia Samborowska Wojciech Błogowski Tadeusz Sulikowski Andrzej Białek Iwona Zawada Krzysztof Dąbkowski Joanna Mitrus Jakub Karczmarski Patrycja Cybula Agnieszka Paziewska Teresa Starzyńska

Background/Objectives: Crohn’s disease is known for being associated with an abnormal composition of the bacterial flora, dysbiosis and intestinal function disorders. Metabolites produced by gut microbiota play a pivotal role in the pathogenesis of CD, and the presence of unspecific extraintestinal manifestations. Methods: The aim of this study was a determination of the level of bacterial metabolites in blood plasma in patients with Crohn’s disease. CD patients (29) and healthy individuals (30) were recruited for this study. Bacterial metabolites (SCFAs and TMAO panel) were measured by a liquid chromatography–mass spectrometry system. Results: A significant correlation (p-value < 0.05) between CD and bacterial metabolites was obtained for three of eight tested SCFAs; acetic acid (reduced in CD; FC 1.7; AUC = 0.714), butyric acid (increased; FC 0.68; AUC = 0.717), 2MeBA (FC 1.168; AUC = 0.702), and indoxyl (FC 0.624). The concentration of CA (FC 0.82) and choline (FC 0.78) in plasma was significantly disturbed according to the biological treatment. Choline level (FC 1.28) was also significantly disturbed in the patients treated with glucocorticoids. In total, 68.97% of Crohn’s patients presented extraintestinal manifestations (EIMs) of Crohn’s disease, mainly osteoarticular complications. The level of BA was statistically significantly elevated in patients with extraintestinal (FC 0.602) manifestations, while in the group of patients with osteoarticular complications, a significant difference in the level of betaine (FC 1.647) was observed. Conclusions: The analyzed bacterial metabolites of plasma may significantly help in the diagnostic process, and in the monitoring of the disease course and treatment, in a lowly invasive way, as biomarkers after additional research on a larger group of patients.

Nutrients doi: 10.3390/nu17010073

Authors: Fortuna Iannuzzo Elisabetta Schiano Maria Maisto Anna Schettino Noemi Marigliano Anella Saviano Adel Abo Mansour Asif Jilani Iqbal Francesco Maione Gian Carlo Tenore Ettore Novellino

Background/Objectives: Benign prostatic hyperplasia (BPH) is one of the most common chronic diseases affecting the urinary tract that occurs mainly in men over 40 years of age. Among the natural therapies, proanthocyanidins (PACs), which can treat a wide range of immune-mediated inflammatory diseases (IMIDs), have been shown to play an important role in the treatment of pathologies concerning prostate health. In this regard, the present study aimed to evaluate the different bioactivities of a grape seed extract (GSE), rich in polymeric PACs, and its version processed under alkaline conditions (ATGSE), characterized by a higher content of oligomeric PACs, in an animal model of BPH induced by subcutaneous injection of testosterone (1 mg/mouse). Methods: These latter were divided into a control group (vehicle, olive oil), a BPH group (testosterone 1 mg/mouse), and four treatment groups treated with GSE (500 mg/kg) and ATGSE (125, 250, 500 mg/kg) by oral gavage. At the experimental endpoint (4 weeks), hematological and biochemical analyses of blood and tissues were performed. Results: Data showed that oral administration of ATGSE (250 mg/kg) was significantly more effective than GSE in reducing prostate (p ≤ 0.0001) and seminal vesicle (p ≤ 0.0001) weight. Moreover, ATGSE exhibited enhanced effectiveness in significantly reducing PSA levels (p ≤ 0.0001 vs. GSE) and the expression of key pro-inflammatory cyto-chemokines in prostate and seminal vesicles homogenates. Conclusions: These findings pave the way for the clinical application of ATGSE as a nutraceutical and/or functional food.

Nutrients doi: 10.3390/nu17010072

Authors: Fatima Maqoud Francesco Maria Calabrese Giuseppe Celano Domenica Mallardi Francesco Goscilo Benedetta D’Attoma Antonia Ignazzi Michele Linsalata Gabriele Bitetto Martina Di Chito Pasqua Letizia Pesole Arianna Diciolla Carmen Aurora Apa Giovanni De Pergola Gianluigi Giannelli Maria De Angelis Francesco Russo

Aims: This study explores the link between body mass index (BMI), intestinal permeability, and associated changes in anthropometric and impedance parameters, lipid profiles, inflammatory markers, fecal metabolites, and gut microbiota taxa composition in participants having excessive body mass. Methods: A cohort of 58 obese individuals with comparable diet, age, and height was divided into three groups based on a priori clustering analyses that fit with BMI class ranges: Group I (25–29.9), Group II (30–39.9), and Group III (>40). Anthropometric and clinical parameters were assessed, including plasma C-reactive protein and cytokine profiles as inflammation markers. Intestinal permeability was measured using a multisaccharide assay, with fecal/serum zonulin and serum claudin-5 and claudin-15 levels. Fecal microbiota composition and metabolomic profiles were analyzed using a phylogenetic microarray and GC-MS techniques. Results: The statistical analyses of the clinical parameters were based on the full sample set, whereas a subset composed of 37 randomized patients was inspected for the GC/MS metabolite profiling of fecal specimens. An increase in potentially pro-inflammatory bacterial genera (e.g., Slackia, Dorea, Granulicatella) and a reduction in beneficial genera (e.g., Adlercreutzia, Clostridia UCG-014, Roseburia) were measured. The gas chromatography/mass spectrometry analysis of urine samples evidenced a statistically significant increase in m-cymen-8-ol, 1,3,5-Undecatriene, (E, Z) and a decreased concentration of p-cresol, carvone, p-cresol, and nonane. Conclusions: Together, these data demonstrated how an increased BMI led to significant changes in inflammatory markers, intestinal barrier metabolites, glucose metabolism, endocrine indicators, and fecal metabolomic profiles that can indicate a different metabolite production from gut microbiota. Our findings suggest that _targeting intestinal permeability may offer a therapeutic approach to prevent and manage obesity and related metabolic complications, reinforcing the link between gut barrier function and obesity.

Nutrients doi: 10.3390/nu17010071

Authors: Shuanggou Zhang Yuanfei Yang Ruohan Zhang Jian Gao Mengyun Wu Jing Wang Jun Sheng Peiyuan Sun

Background: Alpiniae oxyphyllae Fructus (AOF) is a medicinal and edible resource that holds potential to ameliorate hyperuricemia (HUA), yet its mechanism of action warrants further investigation. Methods: We performed network pharmacology, molecular docking, molecular dynamics simulation, and in vitro experiments to investigate the potential action and mechanism of AOF against HUA. Results: The results indicate that 48 potential anti-HUA _targets for 4 components derived from AOF were excavated and predicted through public databases. Gene Ontology (GO) enrichment analysis indicated that there are 190 entries related to biological process, 24 entries related to cellular component, 42 entries related to molecular function, and 44 entries related to Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathways. The results of molecular docking showed that the main active ingredients of AOF may have potential therapeutic effects on immune system disorders and inflammation caused by HUA by binding to _targets including peroxisome-proliferator-activated receptor gamma (PPARG), estrogen receptor 1 (ESR1), prostaglandin G/H synthase 2 (PTGS2), and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR). Subsequently, we further determined the stability of the complex between the core active ingredient and the core _target proteins by molecular dynamics simulation. The results of cell experiments demonstrated that stigmasterol as the core active ingredient derived from AOF significantly upregulated the expression levels of ESR1 and PPARG (p < 0.001) to exert an anti-HUA effect. Conclusions: In summary, we have systematically elucidated that the mechanism of main active ingredients derived from AOF mainly exert their pharmacological effects by acting on multiple _targets in this study. Our studies will provide a scientific basis for the precise development and utilization of AOF.

Nutrients doi: 10.3390/nu17010070

Authors: Christopher P. F. Marinangeli

The creation of sustainable food systems is a “wicked problem”; it is multifaceted with no single or one-size-fits-all solution [...]

Nutrients doi: 10.3390/nu17010069

Authors: Summer R. Wilmoth Leah L. Carrillo-McCracken Bradley Wilhite Meixia Pan Deborah Parra-Medina Erica T. Sosa Ramon Reyes Meizi He

Background/Objectives: This study aimed to adapt evidence-based diabetes self-management education and support (DSMES) into a faith-based (FB) context for Hispanic communities and compare its effectiveness to a faith-placed (FP) approach using the church as a venue for DSMES delivery. Methods: A cluster-randomized trial was conducted among adults with type 2 diabetes from predominantly Hispanic churches. The churches were assigned to either the FB Group (nine churches, n = 146) or the FP Group (seven churches, n = 125). The FB Group, led by trained lay health leaders, received a health sermon, a six-session DSMES program, and a seven-session Healthy Bible Study. The FP Group, led by outside health professionals, received the same six-session DSMES and a seven-session partial attention control curriculum. Key outcomes, including hemoglobin A1c (HbA1c), waist circumference (WC), diabetes distress, self-care activities, and self-efficacy, were assessed at baseline, 6, 9, and 12 months. Results: The FB Group had lower HbA1c levels than the FP Group at 6 months (−0.3%, p < 0.01), with no within-group differences post-intervention. No significant between-group differences were found for other outcomes. Within-group comparisons from baseline showed that both groups reduced WC at 9 and 12 months. Both groups showed reductions in diabetes distress and increased self-efficacy at all time points post-intervention (p < 0.05). The FB Group increased self-care scores at all time points post-intervention, while the FP Group increased at 9 and 12 months. Conclusions: DSMES can be effectively delivered in church settings by trained lay leaders or health professionals in Hispanic communities. Adding a spiritual dimension to DSMES may enhance outcomes.

Nutrients doi: 10.3390/nu17010068

Authors: Renata Korycka-Bloch Pawel Balicki Agnieszka Guligowska Bartlomiej K. Soltysik Tomasz Kostka Zuzanna Chrzastek

Objectives: The aim of this study was to evaluate which anthropometric index, either body mass index (BMI) or weight-adjusted waist index (WWI), is more accurately associated with the prevalence of the most common chronic diseases and components of geriatric assessment in hospitalized older adults. Methods: The study included a total of 2945 hospitalized older adults (median age 82 years). The associations between the presence of chronic diseases and Comprehensive Geriatric Assessment (CGA) results were compared with WWI and BMI values. Results: The WWI was significantly higher in both sex groups suffering from hypertension, diabetes, osteoarthritis, and depression. In women, the parameter was increased among individuals with previous myocardial infarction, who presented heart failure symptoms or had chronic kidney disease diagnosed, whereas in men, among those with pulmonary diseases and osteoporosis, WWI was related to many CGA parameters oftentimes where BMI proved to fail. There was a positive correlation of WWI with the presence of depressive symptoms assessed with the geriatric depression scale (GDS) but no significant correlation with BMI. In multiple logistic regression models, WWI was a stronger predictor of depression as compared to waist circumference or the waist-to-height ratio. Conclusions: There is an association between a higher WWI and depression diagnosis as well as the presence of depressive symptoms according to the GDS in hospitalized older adults, both women and men. There is no such correlation between depression and BMI. Both high BMI and high WWI values seem to identify older patients with cardiometabolic diseases such as hypertension and diabetes. According to this study, WWI seems to be a promising indicator of depression risk and, similarly to BMI, a useful parameter for the assessment of cardiometabolic risk in older hospitalized adults.

Nutrients doi: 10.3390/nu17010067

Authors: Jose Antonio Brandi Antonio Shawn M. Arent Darren G. Candow Guillermo Escalante Cassandra Evans Scott Forbes David Fukuda Maureen Gibbons Patrick Harty Andrew R. Jagim Douglas S. Kalman Chad M. Kerksick Jennifer A. Kurtz Joseph Lillis Lonnie Lowery Gianna F. Mastrofini Scotty Mills Michael Nelson Flavia Pereira Justin Roberts Michael Sagner Jeffrey Stout Jaime Tartar Adam Wells

Energy drinks are a commonly consumed beverage, and studies suggest a possible performance-enhancing effect. A Google Scholar search using the keywords “energy drinks” and “exercise” yields numerous results, underscoring the voluminous research on this topic. However, there are questions regarding the effectiveness and safety of energy drinks. These questions include, but are not limited to: (1) What are the main active ingredients in energy drinks? (2) Do energy drinks assist in weight management? (3) Do energy drinks enhance aerobic performance? (4) Do energy drinks enhance athletic speed? (5) Do energy drinks improve reaction time? (6) Do energy drinks enhance lean tissue mass? (7) Can energy drinks improve cognitive performance? (8) Does the acute consumption of energy drinks elevate resting energy expenditure? (9) Is there any evidence to suggest that energy drinks are more effective than an identical serving of caffeine alone? (10) Are there sex differences in the response to energy drink consumption? (11) Do energy drinks affect sleep or sleepiness? (12) Should pregnant women avoid energy drinks? (13) Do energy drinks adversely affect cardiovascular function? (14) Does consuming energy drinks cause brain damage? (15) What are other safety considerations regarding energy drinks? (16) Is there any evidence to suggest that energy drinks are more effective than an identical serving of caffeine alone? (17) If caffeine is the main active ingredient in energy drinks and coffee, why is there a discrepancy in the adverse events reported for each? To address these questions, we performed an evidence-based scientific evaluation of the literature on energy drink supplementation.

Nutrients doi: 10.3390/nu17010066

Authors: Mira Dewi Nuri Andarwulan Utami Wahyuningsih Renata Kazimierczak Dominika Średnicka-Tober

Background: A number of clinical studies have shown a positive association between the maternal n-3 PUFA status during pregnancy and fetal and newborn development and health. Despite this well-documented role of n-3 PUFAs in pregnancy, data on maternal the LC-PUFAs status during pregnancy in the Indonesian population, to our knowledge, are not yet available. This study reports on the LC-PUFA dietary intake among pregnant women in a suburban population of Bogor City, West Java, Indonesia. It also explores the associations of maternal LC-PUFA intake with maternal blood, cord blood, and breast milk LC-PUFA levels and the associations of the latter with infant body composition. Methods: A total of 142 pregnant women and 104 newborn infants were included in this study. The dietary intake of energy, macronutrients, and selected LC-PUFAs (LA, ALA, EPA, and DHA) was assessed by 2 × 24 h food recall and FFQ. LC-PUFA levels were measured in maternal blood, cord blood, and breast milk. Newborn body composition was determined by anthropometric measures. Results: The study found that the inadequate intake of energy, protein, and carbohydrates was highly prevalent among pregnant women subjects. The intake of the most important n-3 PUFAs (ALA, EPA, and DHA) was far below the recommended values. Maternal dietary DHA intakes were negatively associated with birth weight and infant fat mass at birth, and dietary intake of total fat and n-6 LA were associated with increased fat accumulation in newborns at specific body sites. Moreover, positive correlations were identified between the EPA in maternal blood and infant % fat mass, and between the DHA in cord blood and newborn birth weight. Conclusions: Further longitudinal studies, including clinical and biomolecular analyses, are suggested to be conducted to monitor maternal and child health and nutrition in Indonesia and develop well-attuned intervention strategies.

Nutrients doi: 10.3390/nu17010064

Authors: Dominika Skolmowska Dominika Głąbska Dominika Guzek Jakub Grzegorz Adamczyk Hanna Nałęcz Blanka Mellová Katarzyna Żywczyk Krystyna Gutkowska

Background: Nutrition knowledge may be translated into adequate dietary intake and proper eating habits, so adolescent education programs focusing on improving eating habits and nutrition knowledge are needed. The aim of the cross-sectional study with pair-matched controls was to assess the Consumer Nutrition Knowledge Scale (CoNKS) results and its determinants after one year of intervention in a national extracurricular athletics program within a pair-matched sample of Polish adolescents. Methods: The #goathletics Study evaluated a Polish national extracurricular athletics program, ‘Athletics for all’, being a voluntary and free-of-charge physical activity program organized by the Polish Athletics Association. The study allowed comparing the intervention group of adolescents aged 10–14 years, participating in the program for at least 9 months (a school year) and a pair-matched group not participating in it, while the matching was based on city, gender, and age (each group: n = 506 adolescents, n = 281 females and n = 225 males). The nutrition knowledge was assessed using a Consumer Nutrition Knowledge Scale (CoNKS), and during the analysis, the following factors were taken into account: body weight, height, Body Mass Index (BMI), waist circumference, and waist-to-height ratio (WHtR). Results: The ‘Athletics for all’ program participation influenced not only the total CoNKS score (p < 0.0001) but also the scores in all studied areas—within procedural nutrition knowledge (p = 0.0002), declarative nutrition knowledge on nutrients (p = 0.0001), and declarative nutrition knowledge on calories (p < 0.0001), and program participants revealed a stronger understanding of all the studied areas compared to non-participating individuals. Gender, BMI, and central obesity tendency were not associated with the total CoNKS score (p > 0.05), or any of the studied areas (p > 0.05). The statistically significant differences in the number of correct answers were observed for four items within procedural nutrition knowledge, for four items within declarative nutrition knowledge on nutrients, and for four items within declarative nutrition knowledge on calories (p < 0.05), while for all of them, ‘Athletics for all’ program participants revealed a stronger understanding. Conclusions: One year of intervention in a national extracurricular athletics program significantly influenced the nutrition knowledge of the studied group of adolescents aged 10–14 years. While compared with the pair-matched control group of Polish adolescents, they were characterized by a stronger understanding of all areas of nutrition knowledge.

Nutrients doi: 10.3390/nu17010065

Authors: Chatnarong Putthong Thanasorn Panmanee Pensri Charoensit Sukunya Ross Kongaphisith Tongpoolsomjit Jarupa Viyoch

Background/Objectives: UV radiation is a primary cause of skin damage and photoaging. β-carotene, a potent antioxidant, aids in mitigating UV-induced oxidative stress and enhancing skin photoprotection. This research aimed to evaluate the efficacy of a nutraceutical product designed to prevent photoaging. Methods: The product consists of a blend of hemp seed oil and banana (Musa AA), formulated as a chewable tablet. Healthy male participants aged 35–50 years were enrolled in a randomized, parallel, single-blind, placebo-controlled clinical trial. Participants received either the chewable nutraceutical (five tablets after meals in the morning and evening, equivalent to 8 ± 2 mg/day of β-carotene and 400 mg/day of PUFA) or a chewable placebo for 16 weeks. A total of thirty-six participants successfully completed the entire 16-week study. Results: Administration of the nutraceutical resulted in a significant reduction (p < 0.05) in UV solar light stimulator-induced erythema on the dorsal skin at week 4, with a mean value of 3.76 ± 0.46 AU, compared to the initial value of 4.88 ± 0.62 AU at week 0. Additionally, serum β-carotene concentration significantly increased from 0.45 ± 0.02 µg/mL at week 0 to 0.61 ± 0.06 µg/mL at week 16 (p < 0.05). Moreover, skin intensity in the sun-exposed arm area also significantly improved at week 16, increasing from 71.33 ± 3.50 at week 0 to 81.80 ± 4.45 (p < 0.05). Conclusions: The results indicate that the developed nutraceutical may offer effective protection against erythema, making it a promising option for preventing photoaging.

Nutrients doi: 10.3390/nu17010063

Authors: Jekaterina Neteca Una Veseta Inga Liepina Katrina Volgemute Maija Dzintare Dmitry Babarykin

Background/Objectives: This study addresses the growing interest in nutritional supplements that improve athletic performance in endurance sports. Previous research suggests that nitrates in beetroot juice enhance blood vessel dilation and oxygen delivery to muscles. However, the effects of these nitrates on cardiopulmonary performance in female athletes remain underexplored. The aim of this study was to evaluate the effect of beetroot juice supplementation on aerobic work capacity in female endurance athletes. Methods: A cardiopulmonary exercise test (CPET) was conducted to assess aerobic work capacity. Eighteen healthy female endurance athletes (22.9 ± 5.6 years) participated in the study. The participants were randomly assigned to two groups: the control group (placebo group n = 9), which received a nitrate-free placebo beverage, and the experimental group (beetroot juice group n = 9), which consumed 50 mL of beetroot juice concentrate (~6.2 mmol nitrate) two and a half hours before the second test. Results: The results showed that the beetroot juice group demonstrated significant improvements in minute ventilation (VE), respiratory equivalents (VE/VO2 and VE/VCO2), and heart rate (HR) (p < 0.05). Maximal oxygen consumption (VO2 max) increased by 4.82% in the beetroot juice group (from 35.24 ± 5.07 to 36.94 ± 4.91 mL·min−1·kg−1), whereas a small decrease was observed in the placebo group. Conclusions: These findings indicate that beetroot juice may be an effective ergogenic aid, enhancing oxygen utilization and energy production during exercise in female athletes. In terms of practical applications, beetroot juice could contribute to improved athletic performance and serve as a valuable addition to athletes’ nutritional plans. Future studies should explore the long-term effects, optimal dosages, and duration of supplementation in larger and more diverse populations.

Nutrients doi: 10.3390/nu17010061

Authors: Olga Czerwińska-Ledwig Alicja Nowak-Zaleska Małgorzata Żychowska Katarzyna Meyza Tomasz Pałka Adrianna Dzidek Agata Szlachetka Artur Jurczyszyn Anna Piotrowska

Background: The physical activity of different groups of individuals results in the rearrangement of microbiota composition toward a symbiotic microbiota profile. This applies to both healthy and diseased individuals. Multiple myeloma (MM), one of the more common hematological malignancies, predominantly affects older adults. Identifying an appropriate form of physical activity for this patient group remains a challenge. The aim of this study was to investigate the impact of a 6-week Nordic walking (NW) training program combined with a 10/14 time-restricted eating regimen on the gut microbiota composition of multiple myeloma patients. Methods: This study included healthy individuals as the control group (n = 16; mean age: 62.19 ± 5.4) and patients with multiple myeloma in remission (MM group; n = 16; mean age: 65.00 ± 5.13; mean disease duration: 57 months). The training intervention was applied to the patient group and consisted of three moderate-intensity sessions per week, individually tailored to the estimated physical capacity of each participant. The taxonomic composition was determined via 16S rRNA sequencing (V3–V9 regions). The microbiota composition was compared between the patient group and the control group. Results: The alpha and beta diversity metrics for species and genus levels differed significantly between the control and patient groups before the implementation of the NW program. In contrast, no differences were observed between the control and patient groups after the training cycle, indicating that the patients’ microbiota changed toward the pattern of the control group. This is confirmed by the lowest values of average dissimilarity between the MMB groups and the control at all taxonomic levels, as well as the highest one between the control group and the MMA patient group. The gut microbiota of the patients was predominantly represented by the phyla Firmicutes, Actinobacteria, Verrucomicrobia, Proteobacteria, and Bacteroidetes. Conclusions: The training, combined with time-restricted eating, stimulated an increase in the biodiversity and taxonomic rearrangement of the gut microbiota species.

Nutrients doi: 10.3390/nu17010062

Authors: Giuliana Favara Andrea Maugeri Martina Barchitta Erika Lanza Roberta Magnano San Lio Antonella Agodi

Background/Objectives: Breast milk is a dynamic, personalized nutrition source, influenced by maternal diet, lifestyle, and environmental factors, which shape its composition and impact infant health. This review synthesizes evidence on the associations between maternal lifestyles (e.g., diet, physical activity, smoking), breast milk composition, and child health, offering insights for interventions to optimize breastfeeding benefits. Methods: We searched Web of Science, Medline, Embase, and PubMed for studies published up to March 2024 using predefined terms. Results: Out of 5244 articles, 20 studies met the inclusion criteria. Maternal Body Mass Index and macronutrient intake significantly affected breast milk fatty acid composition, influencing infant growth, cognitive development, and metabolic health. Micronutrient intake, particularly iodine, omega-3 fatty acids, and vitamins, was linked to better neurodevelopment and reduced atopic risks. Maternal diet and supplementation improved breast milk nutrient profiles and infant outcomes, though exposure to toxins like ochratoxin A raised concerns. Smoking was associated with altered milk composition, including lower osteopontin levels, potentially affecting infant immunity and growth. Conclusions: This review emphasizes that adequate intake of key nutrients is essential for infant development, highlighting the need for policies that address nutritional deficiencies, promote healthy lifestyles, and reduce socio-economic barriers. These efforts can improve outcomes for both mothers and children, enhancing public health and reducing disparities.

Nutrients doi: 10.3390/nu17010060

Authors: Sandi Assaf Owen Kelly

Background/Objectives: Youthful, smooth skin is highly desired in modern society. Individuals invest in cosmetics, plastic surgeons, and dermatologists in pursuit of perfect skin. However, many do not seek out dietary changes to improve skin health. Although research has been conducted on the role of nutrition and select nutrients and phytonutrients on skin health, there is a lack of healthy food recommendations for clear skin. Methods: The literature was assessed to determine which nutrients and phytonutrients play a significant role in the protection and maintenance of skin health. Key compounds were highlighted as there is evidence to suggest they have a significant role in skin health: vitamin A, vitamin C, vitamin D, vitamin E, zinc, omega-6 and omega-3 fatty acids, polyphenols/flavonoids, copper, selenium, and silicon. USDA FoodData Central and FooDB (food database), were utilized to select foods and food groups containing the key nutrients and phytonutrients. Results: A skin-healthy dietary pattern is proposed in addition to a scoring system to assess diet. A sample skin-healthy daily diet was designed, using only whole foods, that met the Daily Values for vitamins and minerals and contained key compounds for skin health. Conclusions: There is a clear link between nutrition and skin health, or nutritional dermatology; however, more research needs to be done to find the intersection between both disciplines.

Nutrients doi: 10.3390/nu17010059

Authors: Młynarska Kustosik Mejza Łysoń Delebis Orliński Rysz Franczyk

This narrative review explores the benefits and risks of cannabinoids in kidney health, particularly in individuals with pre-existing renal conditions. It discusses the roles of cannabinoid receptor ligands (phytocannabinoids, synthetic cannabinoids, and endocannabinoids) in kidney physiology. The metabolism and excretion of these substances are also highlighted, with partial elimination occurring via the kidneys. The effects of cannabinoids on kidney function are examined, emphasizing both their potential to offer nephroprotection and the risks they may pose, such as cannabinoid hyperemesis syndrome and ischemia-reperfusion injury. These complexities underscore the intricate interactions between cannabinoids and renal health. Furthermore, this review highlights the association between chronic synthetic cannabinoid use and acute kidney injury, stressing the need for further research into their mechanisms and risks. This article also highlights the growing prevalence of edible cannabis and hemp seed consumption, emphasizing their nutritional benefits, legal regulations, and challenges such as inconsistent labeling, potential health risks, and implications for kidney health. The review delves into the roles of CB1 and CB2 receptors in diabetic nephropathy, chronic kidney disease, and obesity-related kidney dysfunction, discussing the therapeutic potential of CB2 agonists and CB1 antagonists. Additionally, it examines the potential diuretic and anti-inflammatory effects of cannabinoids in preventing kidney stones, suggesting that cannabinoids could reduce crystal retention and lower the risk of stone formation. Cannabinoids’ effects on kidneys depend heavily on the characteristics of individual substances, as synthetic cannabinoids pose a major threat to the health of users. Cannabinoids offer therapeutic potential but require more research to confirm their benefits. Distinguishing between therapeutic cannabinoids and harmful synthetic variants is crucial for safe clinical application.

Nutrients doi: 10.3390/nu17010056

Authors: Marlene A. T. Vijver Nils Bomer Robert C. Verdonk Peter van der Meer Dirk J. van Veldhuisen Olivier C. Dams

Background: Micronutrient deficiencies are common and play a significant role in the prognosis of many chronic diseases, including heart failure (HF), but their prevalence in HF is not well known. As studies have traditionally focused on causes originating within the intestines, exocrine pancreatic insufficiency (EPI) has been overlooked as a potential contributor. The exocrine pancreas enables the absorption of various (fat-soluble) micronutrients and may be insufficient in HF. We hypothesize that EPI contributes to micronutrient deficiencies in HF. Objectives: To evaluate micronutrient concentrations in HF cases and their association with clinical characteristics and EPI. Materials and Methods: Plasma samples from 59 consecutive hospitalized patients with HF were analyzed for vitamins A, D, and E and the minerals selenium and zinc. EPI was defined as fecal elastase 1 level < 206 μg/g. Results: The mean age of patients was 59 ± 14 years, with 24 (41%) being women, and a median NT-proBNP concentration of 3726 [2104–6704] pg/mL was noted. Vitamin A deficiency occurred in eight (14%) of the patients, and 12 (20%) exceeded the upper limit. More than half (51%) were vitamin D-deficient. No patients showed vitamin E deficiency, but 14 (24%) had elevated levels. Selenium deficiency was common, affecting 36 (61%) patients, while zinc was below the normal range in seven patients (12%). Micronutrient levels did not differ significantly based on the presence of EPI. Conclusions: This study provides novel insights into the micronutrient status of patients with HF. Deficiencies in vitamins A and D, selenium, and zinc are prevalent in HF, but these findings are not associated with exocrine pancreatic function.

Nutrients doi: 10.3390/nu17010057

Authors: Chhiti Pandey Alison Maunder Jing Liu Vaishnavi Vaddiparthi Michael F. Costello Mahnaz Bahri-Khomami Aya Mousa Carolyn Ee

Background and Objectives: Nutrient supplements are commonly used to improve fertility outcomes by women with infertility trying to conceive spontaneously or utilising medically assisted reproduction (MAR). However, despite their widespread use and perceived safety, there is a lack of clear guidance on the efficacy and safety of these supplements for female infertility. The aim of this umbrella review was to identify the best available and most recent evidence on the efficacy and safety of nutrient supplements for female infertility to provide evidence-based guidance for clinicians and reproductive couples. Methods: Five electronic databases were searched for umbrella reviews, meta-analyses, and systematic reviews of randomised controlled trials on nutrient supplements for female infertility, published from August 2017 to January 2024. The primary outcomes were live birth, and clinical and biochemical pregnancy rates. Secondary outcomes were adverse effects including miscarriage and ectopic or multiple pregnancy. Quality assessment was performed using the A MeaSurement Tool to Assess systematic Reviews Version 2.0 (AMSTAR 2), and the certainty of evidence for outcomes were assessed using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach, where possible. Results: Four meta-analyses were included. Multiple micronutrients and antioxidants increased live birth rates in women utilising MAR and/or trying to conceive spontaneously compared to placebo, standard or no treatment (odds ratio (OR) 2.59 and 1.81 respectively) with very low certainty evidence. L-carnitine, coQ10, melatonin, myo-inositol, NAC and vitamin D increased clinical pregnancy rates in women with PCOS and/or undergoing MAR compared to placebo, standard or no treatment (odds ratio (OR) 11.14, 2.49, 1.66, relative risk (RR) 1.52, OR 2.15, and 1.49 respectively) with very low certainty evidence. Vitamin D did not increase biochemical pregnancy rates in women utilising MAR with very low certainty evidence. NAC, vitamin D, and pooled antioxidants had no effect on miscarriage rates or multiple pregnancy rates in women trying to conceive spontaneously or utilising MAR, with low to very low certainty evidence. Pooled antioxidants had no effect on ectopic pregnancy rates in women trying to conceive spontaneously or utilising MAR, with low certainty evidence. Conclusions: The available evidence is insufficient to recommend nutrient supplementation to improve female infertility in women trying to conceive naturally and those utilising MAR. However, there is currently no indication that these nutrients pose any risk of significant harm. Registration: PROSPERO (CRD42022365966) 20 October 2022.

Nutrients doi: 10.3390/nu17010058

Authors: Holly E. Clarke Neda S. Akhavan Taylor A. Behl Michael J. Ormsbee Robert C. Hickner

Background/Objectives: A pilot study was conducted to investigate the effect of four weeks of creatine monohydrate (CrM) on vascular endothelial function in older adults. Methods: In a double-blind, randomized crossover trial, twelve sedentary, healthy older adults were allocated to either the CrM or placebo (PL) group for four weeks, at a dose of 4 × 5 g/day for 5 days, followed by 1 × 5 g/day for 23 days. Macrovascular function (flow-mediated dilation [FMD%], normalized FMD%, brachial-ankle pulse wave velocity [baPWV], pulse wave analysis [PWA]), microvascular function (microvascular reperfusion rate [% StO2/sec]), and biomarkers of vascular function (tetrahydrobiopterin [BH4], malondialdehyde [MDA], oxidized low-density lipoprotein [oxLDL], glucose, lipids) were assessed pre- and post-supplementation with a four-week washout period. Results: CrM significantly increased FMD% (pre-CrM, 7.68 ± 2.25%; post-CrM, 8.9 ± 1.99%; p < 0.005), and normalized FMD% (pre-CrM, 2.57 × 10−4 ± 1.03 × 10−4%/AUCSR; post-CrM, 3.42 × 10−4 ± 1.69 × 10−4%/AUCSR; p < 0.05), compared to PL. Microvascular reperfusion rates increased following CrM (pre-CrM, 2.29 ± 1.42%/sec; post-CrM, 3.71 ± 1.44%/sec; p < 0.05), with no change following PL. A significant reduction in fasting glucose (pre-CrM, 103.64 ± 6.28; post-CrM, 99 ± 4.9 mg/dL; p < 0.05) and triglycerides (pre-CrM, 99.82 ± 35.35; post-CrM, 83.82 ± 37.65 mg/dL; p < 0.05) was observed following CrM. No significant differences were observed for any other outcome. Conclusions: These pilot data indicate that four weeks of CrM supplementation resulted in favorable effects on several indices of vascular function in older adults.

Nutrients doi: 10.3390/nu17010055

Authors: Qin Sun Jieping Wang Huanyu Wang Hanhan Yu Kang Wan Fuyi Ma Ru Wang

Background: Taurine has been demonstrated to regulate and improve metabolic health. However, physiological and pathological differences among individuals with overweight or obesity may result in varied responses to taurine supplementation. This study aims to estimate the effects of long-term taurine supplementation on blood lipids, glycemia, and insulin sensitivity in adults with overweight or obesity through a systematic review and meta-analysis. Methods: The literature search was based on six databases (Web of Science, PubMed, Scopus, EMBASE, Cochrane, and SPORTDiscus) up to October 2024. Subgroup analyses were performed based on daily taurine intake dosage (<3 g or 3 g), overweight (BMI 25–29.9 kg/m2), and obesity (BMI ≥30 kg/m2). Results: The final number of studies that met the inclusion criteria was 9 RCTs. The overall analysis showed that taurine supplementation significantly decreased TG (WMD = −0.56 mg/dL, 95% CI: −0.92 to −0.2, p = 0.002, I2 = 63%), TC (WMD = −0.71 mg/dL, 95% CI: −1.17 to −0.25, p = 0.002, I2 = 73%), and fasting insulin (WMD = −2.15 µU/mL, 95% CI: −3.24 to −1.06, p = 0.0001, I2 = 9%). In the subgroup analysis, long-term taurine intake led to BMI improvement in overweight adults (WMD = −1.14 kg/m2, 95% CI: −1.81 to −0.47, p = 0.0008, I2 = 0%). Meanwhile, improvements in HbA1c (WMD = −0.33%, 95% CI: −0.53 to −0.12, p = 0.002, I2 = 16%) and HOMA-IR (WMD = −0.91, 95% CI: −1.74 to −0.08, p = 0.003, I2 = 54%) were observed only in obese participants following taurine supplementation. Additionally, the long-term intake of 3 g of taurine significantly improved HbA1c (WMD = −0.37%, 95% CI: −0.61 to −0.13, p = 0.003, I2 = 0%) and FPG levels (WMD = −7.14 mg/dL, 95% CI: −12.53 to −1.74, p = 0.003, I2 = 70%) in overweight/obesity. Conclusions: Long-term taurine supplementation is particularly effective in improving glycemic control and insulin sensitivity in obesity. Furthermore, higher doses of taurine (3 g per day) demonstrate even greater improvements in glycemic control.

Nutrients doi: 10.3390/nu17010053

Authors: Rita Salvado Cristina Lugones-Sánchez Sandra Santos-Minguez Susana González-Sánchez José A. Quesada Rocío Benito Emiliano Rodríguez-Sánchez Manuel A. Gómez-Marcos Pedro Guimarães-Cunha Jesús M. Hernandez-Rivas Alex Mira Luis García-Ortiz MIVAS Investigators MIVAS Investigators

Background: Recent research highlights the potential role of sex-specific variations in cardiovascular disease. The gut microbiome has been shown to differ between the sexes in patients with cardiovascular risk factors. Objectives: The main objective of this study is to analyze the differences between women and men in the relationship between gut microbiota and measures of arterial stiffness. Methods: We conducted a cross-sectional study in Spain, selecting 180 subjects (122 women, 58 men) aged between 45 and 74. Subjects with arterial stiffness were identified by the presence of at least one of the following: carotid–femoral pulse wave velocity (cf-PWV) above 12 mm/s, cardio–ankle vascular index (CAVI) above nine, or brachial–ankle pulse wave velocity (ba-PWV) above 17.5 m/s. All other cases were considered subjects without arterial stiffness. The composition of the gut microbiome in fecal samples was determined by 16S rRNA sequencing. Results: We found that women have a more diverse microbiome than men (Shannon, p < 0.05). There is also a significant difference in gut microbiota composition between sexes (Bray–Curtis, p < 0.01). Dorea, Roseburia, and Agathobacter, all of them short-chain fatty-acid producers, were more abundant in women’s microbiota (log values > 1, p-value and FDR < 0.05). Additionally, Blautia was more abundant in women when only the subjects with arterial stiffness were considered. According to logistic regression, Roseburia was negatively associated with arterial stiffness in men, while Bifidobacterium and Subdoligranulum were positively related to arterial stiffness. Conclusions: In the Spanish population under study, women had higher microbiome diversity and potentially protective genera. The host’s gender determines the influence of the same bacteria on arterial stiffness. Trial Registration Number: NCT03900338.

Nutrients doi: 10.3390/nu17010054

Authors: Shabnam Jalili-Moghaddam Gael Mearns Lindsay D. Plank El-Shadan Tautolo Elaine Rush

Background: Fructose (50% of sucrose/sugar) is one component of free-sugars and is metabolized to uric acid, which is a known risk factor for gout and metabolic syndrome. Pacific peoples in New Zealand experience a higher prevalence of gout, type 2 diabetes, and overweight/obesity than other ethnic groups. Interestingly, despite having a similar body mass index (BMI), they tend to have a higher proportion of appendicular skeletal muscle mass (ASMM) and less fat than other ethnic groups. Given this context, this study aimed to evaluate the associations between serum uric acid (SUA), free-sugar intake, and ASMM. Methods: In a nested sub-study from the Pacific Islands Families birth-cohort study, 101 boys and 99 girls (all aged 14 and 15 years) self-reported how often they had consumed foods containing sugar in the past month. Anthropometry, body fatness, and ASMM by dual-energy X-ray absorptiometry and metabolic risk factors, including SUA were measured. Results: Overall, 43% of girls and 57% of boys consumed ‘sugary drinks’ twice or more a day. When analyzed by group, ASMM was positively related to SUA for both boys and girls (r = 0.593, p < 0.0001). The effect of the intake of ‘sugary drinks’ on SUA (r = 0.176, p = 0.013) was reduced when ASMM was considered in the relationships. Conclusions: This study shows high SUA levels in Pacific adolescents, with a positive association between ASMM and SUA in both genders. Sugary drink intake was positively associated with SUA in both boys and girls. High ASMM in Pacific people and an increased risk for raised SUA make it important to work with Pacific communities to reduce added sugar intake and adopt integrated, family-based, culturally centered, and life-course approaches to prevent chronic diseases, including gout.

Nutrients doi: 10.3390/nu17010052

Authors: Anallely López-Yerena Victoria de Santisteban Villaplana Lina Badimon Gemma Vilahur Teresa Padro

Probiotics are gaining recognition as a viable strategy for mitigating cardiovascular risk factors. Specifically, recent studies highlight their potential benefits in managing cholesterol levels, blood pressure, and inflammation, which are critical components in the prevention of cardiovascular diseases (CVD). This comprehensive review aims to elucidate the impact of probiotic consumption on major cardiovascular risk factors, including individuals with hypertension, type II diabetes mellitus, metabolic syndrome, hypercholesterolemia, and in secondary prevention in coronary artery disease. Scientific evidence based on human studies suggests that probiotic consumption is associated with positive effects on anthropometric measures, inflammation markers, blood pressure, glucose metabolism markers, lipid profiles, and endothelial function. However, these findings should be interpreted pragmatically and acknowledge the significant variability in results. This variability may be attributed to factors such as probiotic composition (single strain or multiple strains), the characteristics of the delivery matrix (food, capsules, and sachets), the duration of the intervention, the dosage regimen, and baseline health profiles of the participants. Incorporating probiotics as part of a comprehensive and healthy lifestyle approach can be considered a feasible strategy for both the prevention and management of CVD. However, further research is needed on factors influencing the effect of probiotics, such as: (i) optimal probiotic strain(s), (ii) appropriate dosage, (iii) duration of treatment, (iv) optimal delivery vehicle, and (v) sex-specific differences.

Nutrients doi: 10.3390/nu17010051

Authors: Roxana Elena Matran Andra-Mihaela Diaconu Andreea Maria Iordache Daniela Pacurar Cristina Adriana Becheanu

Background/Objectives: Chronic gastrointestinal disorders often involve nutritional management strategies. On the one hand, inflammatory bowel disease (IBD) is a condition in which most of the patients experience frequent diet manipulation in order to obtain long term remission. On the other hand, for celiac disease (CelD), diet is the only known treatment strategy so far, requiring a life-long gluten-free diet. We aimed to evaluate the comparative food-related quality-of-life (FR-QoL) in light of these dietary interventions between these two conditions. Methods: This is a cross-sectional study, involving children aged 7–18 years diagnosed with IBD and CelD. Assessment of this aspect was performed using the self-reported FR-QoL 29 questionnaire. For CelD, the questionnaires were modified with “CelD” instead of “IBD”. Results: Fifty-one patients were included, 17 in each subgroup (Crohn’s disease (CD), ulcerative colitis (UC), and Celd). FR-QoL scores were negatively correlated with age at inclusion (Spearman’s ρ = −0.284, p = 0.04) and also with age at diagnosis (Spearman’s ρ = −0.291, p = 0.038). The scores were significantly lower in the CD group (64.1 ± 13.4) compared with CelD patients (78.6 ± 20.3), p = 0.036 and UC, p = 0.294. For the IBD group, the scores were not influenced by disease activity. Furthermore, we identified a negative significant correlation between anthropometric indices and FR-QoL scores. Conclusions: The burden of dietary intervention is highest for the CD patients, regardless of their disease activity when compared with UC and CelD patients, most probably because of the unpredictable course and fast response to dietary changes. Although it requires incessantly vigilant eating behavior, CelD has apparently become more “manageable” in recent years.

Nutrients doi: 10.3390/nu17010050

Authors: Ioana Adela Ratiu Corina Moisa Luciana Marc Nicu Olariu Cristian Adrian Ratiu Gabriel Cristian Bako Anamaria Ratiu Simona Fratila Alin Cristian Teusdea Mariana Ganea Mirela Indries Lorena Filip

Background/Objectives: Magnesium plays a crucial role in immune function, influencing immunoglobulin synthesis, antibody-dependent cytolysis, and other immune processes. In renal transplant patients, magnesium deficiency is primarily induced by calcineurin inhibitor treatment, through the reduction of magnesium transporter proteins in the renal tubules, leading to magnesium loss. Methods: To assess the correlation between serum magnesium levels and the long-term outcomes of renal graft and transplant recipients, we conducted a retrospective study on 87 patients who have had a transplant for more than 5 years, a period considered immunologically stable. We evaluated laboratory parameters such as glycemia, creatinine, total protein, and C-reactive protein (CRP), as well as demographic data, primary kidney disease, donor type, comorbidities, and infection incidence. Results: This study revealed clinical stability at over 5 years post-transplantation, with no significant differences between the 5–15 and over-15-years groups with regard to major comorbidities, except for HCV infection (p = 0.018). Reduced magnesium levels were associated with impaired renal function (p = 0.017) and inflammatory syndrome (p = 0.012). Viral infections were correlated with living donor grafts (p = 0.05), hypoproteinemia, and decreased eGFR (estimated glomerular filtration rate), while bacterial infections, namely urinary tract infections (UTIs), were linked to reduced eGFR (p = 0.05, p = 0.046). Female patients with hypomagnesemia had a higher incidence of recurrent UTIs (p = 0.03). Conclusions: Hypomagnesemia correlates with increased infection risk in patients who received a renal transplant more than 5 years ago but does not significantly impact glycemic control or cardiovascular health.

Nutrients doi: 10.3390/nu17010049

Authors: Masoud Akbari Aghdam Ana Pagán Joaquín García-Estañ Noemí M. Atucha

Introduction: Numerous epidemiological studies have demonstrated that consuming foods rich in polyphenols and flavonoids can have beneficial effects on various diseases, including arterial hypertension (HTN). Recent research from our laboratory has shown that certain flavonoids exhibit antihypertensive properties in several animal models of HTN. Our objective was to evaluate the effect of Morus alba L. (white mulberry) extracts in an experimental HTN model characterized by nitric oxide (NO) deficiency. Methods: Male Sprague-Dawley rats were divided into four groups: a control group, hypertensive rats treated with an NO synthesis inhibitor (L-NAME) in drinking water for six weeks, L-NAME rats treated with Morus alba L. extract, and L-NAME rats treated simultaneously with captopril. After six weeks of treatment, we measured blood pressure, endothelial vascular function in the aorta, and platelet aggregation function. Results: Morus alba L. extract partially prevented the development of arterial hypertension due to NO deficiency, although it did not completely normalize blood pressure as captopril did. The extract reduced the excessive vasoconstrictor response to phenylephrine in aortic rings and improved vasodilation in response to acetylcholine, with both effects dependent on increased NO production. Morus alba L. extract also reduced the increased platelet aggregation in response to ADP and collagen in hypertensive animals, although it did not fully normalize this function. Conclusions: Morus alba L. extract demonstrates antihypertensive effects, improves vascular reactivity, and reduces platelet aggregation in a model of arterial hypertension. These effects are primarily related to an increase in nitric oxide activity.

Nutrients doi: 10.3390/nu17010048

Authors: Italo Rennan Sousa Vieira Carlos Adam Conte-Junior

The relationship between dietary bioactive compounds and human health has generated significant interest among members of the scientific community and the general public [...]

Nutrients doi: 10.3390/nu17010047

Authors: Jianfan Zhou Shuting Yin Litao Du Xiangli Xue Qiang He Na Zhao Si Chen Xianliang Zhang

Objective: This study aims to explore the independent and combined associations of physical activity (PA) in different domains and inflammatory diet with type 2 diabetes mellitus (T2DM). Methods: Data from 8736 American adults from the NHANES 2007–2016 were used. PA in different domains was assessed using the self-reported Global Physical Activity Questionnaire, and dietary inflammatory index was estimated based on 24 h dietary recalls. T2DM diagnosis was determined by a combination of self-report and laboratory data. A multivariate modified Poisson regression model was used to explore the independent and combined associations of moderate-vigorous intensity physical activity (MVPA) and inflammatory diet with T2DM. Results: PA in the Work MVPA, Recreational MVPA and Total MVPA domains was independently associated with reduced risk of T2DM, and an inflammatory diet was independently associated with elevated risk of T2DM. In the combined analysis, the combination of active and anti-inflammatory within the Work MVPA, Recreational MVPA and Total MVPA fields was associated with the greatest reduced risk of T2DM, and always associated with decreased risk of T2DM in the active group. Conclusions: Our study emphasizes that the combination of active PA and anti-inflammatory diet is closely associated with the reduced risk of T2DM, and suggests the combination of both for the prevention and treatment of T2DM.

Nutrients doi: 10.3390/nu17010046

Authors: Giovanna Verlato Marta Meneghelli Maria Elena Cavicchiolo

Appropriate nutrition is of paramount importance during infancy and childhood, and Parenteral Nutrition (PN), which is the intravenous infusion of nutrients in the elementary form, may be necessary as a supplement or a full replacement for enteral nutrition [...]

Nutrients doi: 10.3390/nu17010045

Authors: Daniela Malta Sam Esfandiari Livia A. Goldraich Johane P. Allard Gary E. Newton

Background: There is compelling evidence of an inverse association between potassium intake and blood pressure (BP). A potential mechanism for this effect may be dietary potassium-mediated augmentation of endothelium-dependent relaxation. To date, studies have investigated potassium intake supplementation over several weeks in healthy volunteers with variable results on vascular function. There is no assessment of the acute vascular effects of potassium supplementation achieved by the ingestion of potassium-rich food in a hypertensive population. Objective: The purpose of this study was to investigate the effect of a high potassium meal on postprandial endothelial function as measured by flow-mediated dilatation (FMD). Methods: We performed an investigator-blinded randomized crossover trial in 33 treated hypertensive individuals. Participants consumed both a high (~2400 mg) and low (~543 mg) K+ meal, separated by a one-week washout period. The primary endpoint was endothelial function as assessed by FMD pre-meal and postprandially at 60 and 120 min. Meals were compared at each time point using the Hills–Armitage approach. Results: 33 individuals were included in the study (48% male, mean age 68). In the fasting state (Baseline), and at 60 min postprandial, radial artery FMD was not significantly different between the participants after consumption of either meal (baseline: high K+ 4.2 ± 2% versus Low K+ 2.6 ± 3%, p = 0.93; 60 min: high K+ 3.8 ± 4% versus Low K+ 4.1 ± 3%, p = 0.69). However, at 120 min, FMD tended to be higher in participants after the high K+ meal (5.2 ± 4.1%) than after the low K+ meal (3.9 ± 4.1%) (p = 0.07). There were no differences in participants’ radial artery diameter and blood flow between meals. Conclusions: This study does not support our hypothesis that a single high K+ meal improves vascular function in individuals with treated hypertension. This does not contradict the clinical evidence relating greater K+ intake with lower BP, but suggests that mechanistic investigations of increased K+ intake through diet alone and its impact on endothelial function as a mediator to reducing BP are complex and not simply due to single nutrient-mediated improvement in vascular function.

Nutrients doi: 10.3390/nu17010044

Authors: Jana Daher Margo Mountjoy Dalia El Khoury

Background/Objectives: Research has shown that athletes often have poor nutritional knowledge, particularly regarding dietary supplements. The purpose of this study was to investigate the effectiveness of an online nutrition education program in improving nutritional and dietary supplement knowledge among varsity athletes at the University of Guelph in Ontario, Canada. Methods: A total of 30 varsity athletes at the University of Guelph were randomized into experimental [n = 18] and control [n = 12] groups. A randomized wait-list controlled intervention was used, where participants in the experimental group were granted access to an online, 4-week nutrition education program, while no program was provided to the control group within the duration of the study. The program covered nutrition topics and focused on sports nutrition and dietary supplementation. Both groups were administered a validated online nutrition and dietary supplement questionnaire at baseline and post-intervention. Results: Mean baseline knowledge scores for the experimental and control groups were 13.78 ± 2.76 and 13.92 ± 2.39, respectively, and were not significantly different [p = 0.888]. Post-intervention scores were 16.28 ± 1.49 and 14.5 ± 1.88 for the experimental and control groups, respectively, [p < 0.05]. There was a significant interaction between the intervention and time on knowledge. Conclusions: These results indicate that this nutrition education program was successful in significantly improving nutritional and dietary supplement knowledge in varsity athletes.

Nutrients doi: 10.3390/nu17010043

Authors: Maria Elionês de Oliveira Araújo Amanda Maria Lira de Lucena Iasmim Leite Fontes Anny Cristine de Araújo Karla Danielly da Silva Ribeiro

Background/Objectives: This systematic review aims to identify diets related to weight gain in pregnant women diagnosed with gestational diabetes mellitus (GDM). Methods: This study was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses, and its protocol was registered on the International Prospective Register of Systematic Reviews (CRD42023432322). The searches used the medical subject headings in the PubMed/MEDLINE, Web of Science, Scopus, and EMBASE databases. Studies were selected, and data were extracted by three researchers. The Newcastle–Ottawa Scale (NOS) and the Joanna Briggs Institute (JBI) tool were used to assess methodological quality. Results: Six articles were included, most of them of the cohort type, with nutritional strategies lasting 2–15 weeks for overweight/obese women, based on the “macronutrient-adjusted diet” and “calorie-adjusted diet”. Only one study addressed dietary counseling in weight management, and none considered the dietary pattern. The gestational weight gain was 4.91–13.8 kg, and a lower weight gain was found in all studies that used the “macronutrient-adjusted diet” nutritional strategy. However, it did not meet the gestational weight gain _targets. Conclusions: Despite the limited number of studies examining the impact of nutritional strategies on weight gain in women with GDM, some research suggests that diets focused on macronutrient adjustment may lead to less weight gain but are not adequate. Therefore, future studies are needed to evaluate which type of nutritional strategies ensure weight gain control during pregnancy.

Nutrients doi: 10.3390/nu17010042

Authors: Catherine J. Andersen Maria Luz Fernandez

Biomarkers constitute a valuable tool to diagnose both the incidence and the prevalence of chronic diseases and may help to inform the design and effectiveness of precision nutrition interventions. Cardiovascular disease (CVD) continues to be the foremost cause of death all over the world. While the reasons that lead to increased risk for CVD are multifactorial, dyslipidemias, plasma concentrations of specific lipoproteins, and dynamic measures of lipoprotein function are strong biomarkers to predict and document coronary heart disease incidence. The aim of this review is to provide a comprehensive evaluation of the biomarkers and emerging approaches that can be utilized to characterize lipoprotein profiles as predictive tools for assessing CVD risk, including the assessment of traditional clinical lipid panels, measures of lipoprotein efflux capacity and inflammatory and antioxidant activity, and omics-based characterization of lipoprotein composition and regulators of lipoprotein metabolism. In addition, we discuss demographic, genetic, metagenomic, and lifestyle determinants of lipoprotein profiles—such as age, sex, gene variants and single-nucleotide polymorphisms, gut microbiome profiles, dietary patterns, physical inactivity, obesity status, smoking and alcohol intake, and stress—which are likely to be essential factors to explain interindividual responses to precision nutrition recommendations to mitigate CVD risk.

Nutrients doi: 10.3390/nu17010041

Authors: Jianye Li Dominika Maria Wilczyńska Małgorzata Lipowska Ariadna Beata Łada-Maśko Bartosz M. Radtke Urszula Sajewicz-Radtke Bernadetta Izydorczyk Taofeng Liu Zitong Wang Junyu Lu Mariusz Lipowski

Background/Objectives: The mechanisms linking eating attitudes to well-being and physical activity objectives have increasingly attracted the attention of researchers in recent years. This research is particularly significant in the context of the COVID-19 pandemic, which has profoundly disrupted eating habits, exercise routines, and psychosocial well-being across the globe. Additionally, these variables are influenced by cultural dimensions, such as individualism in Poland and collectivism in China. These two countries represent distinct approaches to social health and well-being during the pandemic, offering valuable comparative insights into how cultural contexts shape mental and physical health behaviors; Methods: The study included 644 Polish and 690 Chinese participants. It utilized the Psychological Well-Being Scale (PWBS), the Coronavirus Anxiety Scale (CAS), the Eating Attitudes Test (EAT-26), and the Inventory of Physical Activity Objectives (IPAO); Results: The results indicate that both COVID-19 anxiety and eating attitudes fully mediate the relationship between well-being and physical activity objectives. Full mediation implies that the observed relationship between well-being and physical activity objectives operates entirely through the mediators. Notably, the mediating effect of COVID-19 anxiety was observed only in the Chinese sample, highlighting cultural differences in coping mechanisms and societal responses to anxiety. Cultural differences significantly influenced well-being and physical activity objectives, while eating disorders remained unaffected by cultural and social differences. Additionally, a significant positive correlation was found between COVID-19 anxiety, eating attitudes, and health-related physical activity objectives, underscoring the interplay between mental health and physical activity; Conclusions: These findings underscore the importance of addressing anxiety and eating attitudes to enhance well-being and physical activity behaviors. The study provides a strong theoretical basis for _targeted interventions tailored to cultural contexts. Potential limitations include the reliance on self-reported data and differences in demographic characteristics between the Polish and Chinese samples, which may affect generalizability.

Nutrients doi: 10.3390/nu17010040

Authors: Carmen Ortiz-Salguero Marina Romero-Bernal Ángela González-Díaz Elaheh Sobh Doush Carmen del Río Miriam Echevarría Joan Montaner

Hyperhomocysteinemia (HHcy), characterized by elevated homocysteine (HCys) levels, is associated with increased risks of neurovascular diseases such as stroke or hydrocephalus. HHcy promotes oxidative stress, neuroinflammation, and endothelial dysfunction, disrupting the blood–brain barrier and accelerating neurodegeneration. These processes highlight HCys as both a biomarker and a potential therapeutic _target in vascular-related neurological disorders. Current research suggests that polyphenols, known for their antioxidant and anti-inflammatory properties, may reduce HCys levels and offer neuroprotection. Polyphenols have demonstrated effectiveness in modulating oxidative stress and inflammatory pathways triggered by HHcy. These compounds may also upregulate enzymatic functions involved in HCys metabolism, thus reducing neurotoxicity. Furthermore, polyphenol-rich diets, like the Mediterranean diet, have been linked to lower HCys levels and a reduced incidence of neurovascular disorders. This review provides an overview of HHcy’s role in neurovascular pathologies and examines the therapeutic potential of polyphenols in managing HCys levels and preventing HCys-induced neurovascular damage.

Nutrients doi: 10.3390/nu17010039

Authors: Xueting Qi Ronghui Zhang Hailong Zhu Jia Luo Qiuge Zhang Weijing Wang Tong Wang Dongfeng Zhang

Background: Over 1 billion smokers worldwide, one-third of whom have mental and behavioral disorders, exist. However, factors influencing mental and behavioral disorders due to the use of tobacco remain largely unexplored. This study aims to investigate the relationship between dietary iron intake and mental and behavioral disorders due to the use of tobacco. Methods: Using large population cohort data from the UK Biobank (500,000 participants at 22 assessment centers between 2006 and 2010), we employed logistic and Cox regression analyses to explore both cross-sectional and longitudinal associations between dietary iron intake and mental and behavioral disorders due to the use of tobacco. Additionally, we assessed the nonlinear relationship between dietary iron intake and these disorders using restricted cubic spline plots. Results: Logistic regression analysis indicated that dietary iron intake was negatively associated with mental and behavioral disorders due to the use of tobacco. The Cox regression results supported a protective effect of increased dietary iron intake against these disorders. Stratified and sensitivity analyses were consistent with the primary findings. Restricted cubic spline plots revealed a nonlinear relationship between dietary iron intake and mental and behavioral disorders due to the use of tobacco. In the total sample, as well as in both age groups and the male subgroup, the risk reduction rate initially accelerated before slowing down. In contrast, the risk reduction rate in the female group declined rapidly at first and then leveled off. Conclusions: This study demonstrates that dietary iron intake has a protective effect against mental and behavioral disorders due to the use of tobacco, revealing a nonlinear association between these two traits. These findings provide important insights for the profilaxy and treatment of mental and behavioral disorders due to the use of tobacco in the future.

Nutrients doi: 10.3390/nu17010038

Authors: Joshua P. Sutherland Ang Zhou Elina Hyppönen

Background: Falls are a major public health concern. Daily vitamin D supplementation is a proposed fall prevention strategy; however, safety concerns have arisen from some clinical trials showing increased fall risk when using higher vitamin D dosing methods. The relationship between vitamin D and falls may be influenced by factors, such as inflammation, which can alter the balance of essential nutrients like vitamin D and retinol, potentially affecting motor function. We use a genetic epidemiological approach to explore the association of inflammation, vitamin D, and fall risk. Methods: We included 307,082 UK Biobank participants and conducted observational and Mendelian randomization (MR) analyses to investigate associations between 25-hydroxyvitamin D [25(OH)D] and fall risk, with analyses including restriction to participants who had fallen and had inflammation as defined by CRP ≥ 5 mg/L. Results: In the observational analysis, CRP was associated with a higher (per 5 mg/L CRP increase OR = 1.06, 95% CI 1.05–1.07) and 25(OH)D with a lower odds of falls. The association between 25(OH)D concentrations and fall risk was non-linear (p < 0.001), reflecting a plateauing of the association at higher concentrations. There was an interaction between 25(OH)D and CRP on their association with the odds of falls (p = 0.009). In participants with CRP ≥ 5 mg/L, the association was U-shaped, and the fall risk was elevated for both 25(OH)D < 25 nmol/L and ≥ 100 nmol/L (p < 0.004). The association between high 25(OH)D and falls was most pronounced for participants with CRP ≥ 20 mg/L (≥ 100 nmol/L vs. 50–74.99 nmol/L: OR = 2.40, 95% CI, 1.50–3.86). Genetically predicted higher 25(OH)D was not associated with fall risk in the overall population, but a suggestive association with fall risk was seen in participants who had fallen and had CRP > 20 mg/L (926 cases; OR = 1.20, 95% CI, 1.00–1.44). Conclusions: Our study suggests that inflammation might modify the vitamin D and fall risk relationship. Both low and high 25(OH)D levels are associated with more falls in individuals with chronic inflammation, with supporting evidence seen in both observational and MR analyses. This may provide insight into the increased fall risk following high-dose vitamin D supplementation in clinical trials, warranting further research.

Nutrients doi: 10.3390/nu17010037

Authors: Yuki Minagawa-Watanabe Shigekazu Ukawa Tomoe Fukumura Satoe Okabayashi Masahiko Ando Kenji Wakai Kazuyo Tsushita Akiko Tamakoshi

Background: Community-dwelling older adults are at risk of malnutrition due to age-related declines in energy and nutrient intake. While the positive effect of dining companions on energy and nutrient intake has been suggested, evidence remains inconclusive. This study investigated the association between the number of dining companions and energy and nutrient intake, as well as the contribution of specific food groups to higher intake in the presence of dining companions. Methods: This cross-sectional study included 2865 community-dwelling older adults. The number of dining companions was assessed through self-administered questionnaires and categorized into three groups (none, 1, ≥2). Dietary intake was evaluated using a validated food frequency questionnaire, and multivariable regression analyses were conducted to control for potential confounders. Results: Participants dining with two or more companions consumed significantly more energy (β 143.85; 95% CI: 30.05, 257.65; p for trend = 0.01), protein (β 6.32; 95% CI: 1.05, 11.59), fat (β 6.78; 95% CI: 2.44, 11.12; p for trend = 0.002), and carbohydrates (β 17.43; 95% CI: 1.48, 33.37; p for trend = 0.06) compared to those dining alone. They also consumed higher amounts of rice, fats and oils, meat, other vegetables, fruits, and mushrooms. Conclusions: Dining with two or more companions is associated with greater energy and nutrient intake, particularly from energy- and nutrient-dense foods. Encouraging shared meals could serve as a potential approach to support dietary quality and address risks of malnutrition in older adults.

Nutrients doi: 10.3390/nu17010036

Authors: Ines Drenjančević Ana Stupin Ivana Jukić Nikolina Kolobarić Petar Šušnjara Nataša Kozina Lora Kovač Zrinka Mihaljević

Backgrounds/Objectives: Following previous findings on high-salt (HS)-intake-related increase of oxidative stress, this study explored whether carnosine (CAR; β-alanyl-L-histidine), a reactive oxygen species (ROS) scavenger, enhanced antioxidative defence and vascular function following HS, potentially via the NRF2 or HIF-1α signalling pathway. Methods: Sprague Dawley rats (64, 8–10 weeks old, both sexes) were divided into four groups (n = 6/group): CTRL (0.4% NaCl), HS (4% NaCl for 7 days), CTRL + CAR (0.4% NaCl and 150 mg/kg/day oral CAR supplementation), and HS + CAR (4% NaCl and CAR). Acetylcholine-induced relaxation (AChIR) and hypoxia-induced relaxation (HIR) were evaluated in norepinephrine-precontracted (NE, 10−7 M) aortic rings. HIR was also tested with NRF2 (ML-385, 5 × 10−6 M) and HIF-1α (LW6, 10−4 M) inhibitors. Gene expression of superoxide dismutases 1, 2, and 3 (SOD1, 2 and 3), glutathione peroxidases (GPx1 and 4), catalase (CAT), NRF2, and NAD(P)H dehydrogenase (quinone 1) (NQO1) in aortic tissue was measured by RT-qPCR. Ferric reducing antioxidant power (FRAP) and advanced oxidation protein products (AOPPs) assays were performed on serum samples. All experimental procedures conformed to the European Guidelines (directive 86/609) and were approved by the local and national Ethical Committees (#2158-61-46-23-36, EP355/2022). Results: HS impaired AChIR and HIR, both preserved by CAR. NRF2 and HIF-1α inhibitors suppressed HIR in the HS and HS + CAR groups. CAR significantly increased SOD1 and 2, NRF2, and NQO1 expression and SOD activity compared to the CTRL and HS groups. GPx1 and GPx4 were upregulated in HS + CAR compared to HS. CAR prevented an increase in AOPPs, which were elevated in HS, while FRAP was highest in HS + CAR. Conclusions: Carnosine enhances antioxidative defence by upregulating antioxidant enzymes and activities and preserves vascular relaxation, likely via NRF2 signalling.

Nutrients doi: 10.3390/nu17010035

Authors: Feng Wu Yaxiu Guo Yihua Wang Xiukun Sui Hailong Wang Hongyu Zhang Bingmu Xin Chao Yang Cheng Zhang Siyu Jiang Lina Qu Qiang Feng Zhongquan Dai Chunmeng Shi Yinghui Li

Background: Long-term fasting demonstrates greater therapeutic potential and broader application prospects in extreme environments than intermittent fasting. Method: This pilot study of 10-day complete fasting (CF), with a small sample size of 13 volunteers, aimed to investigate the time-series impacts on gut microbiome, serum metabolome, and their interrelationships with biochemical indices. Results: The results show CF significantly affected gut microbiota diversity, composition, and interspecies interactions, characterized by an expansion of the Proteobacteria phylum (about six-fold) and a decrease in Bacteroidetes (about 50%) and Firmicutes (about 34%) populations. Notably, certain bacteria taxa exhibited complex interactions and strong correlations with serum metabolites implicated in energy and amino acid metabolism, with a particular focus on fatty acylcarnitines and tryptophan derivatives. A key focus of our study was the effect of Ruthenibacterium lactatiformans, which was highly increased during CF and exhibited a strong correlation with fat metabolic indicators. This bacterium was found to mitigate high-fat diet-induced obesity, glucose intolerance, dyslipidemia, and intestinal barrier dysfunction in animal experiments. These effects suggest its potential as a probiotic candidate for the amelioration of dyslipidemia and for mediating the benefits of fasting on fat metabolism. Conclusions: Our pilot study suggests that alterations in gut microbiota during CF contribute to the shift of energy metabolic substrate and the establishment of a novel homeostatic state during prolonged fasting.

Nutrients doi: 10.3390/nu17010034

Authors: Yecheng Yao Qiya Guo Caicui Ding Ying Zhou Chao Song Yan Zhang Weiyan Gong Fan Yuan Zheng Chen Tanchun Yu Xinyue Wu Li He

Objective: The objective of this study is to gain insights into the influence of income on the body mass index (BMI) locus in the elderly population. Methods: The Chinese Longitudinal Healthy Longevity and Happy Family Study (CLHLS-HF) was included at baseline (2008) for participants aged 65 years and older. The total number of participants analyzed in this study was 7555. A population-based trajectory model (GBTM) was used. The economic income level was an independent variable and adjusted for age, gender, ethnicity, education, marriage, and physical activity (Model 1), and the baseline BMI value was added in Model 2, with a quadratic of the income added in Model 3. A sensitivity analysis was adopted. Results: Three BMI trajectory groups were identified using GBTM and were labeled as “overweight”, “normal”, and “obesity”. After adjusting for covariates (Model 1), with the increase in economic income, the risk of the elderly transitioning into the overweight trajectory group and the obesity trajectory group was relatively increased. When the baseline BMI values were adjusted for Model 2, the effect of economic income on the overweight and obesity trajectories was enhanced. A sensitivity analysis was performed, and it was found that the result of the positive impact of economic income on the BMI trajectory group was robust. Conclusions: The higher the income, the greater the risk of Chinese elderly individuals developing the overweight or obesity trajectory. It is suggested that elderly individuals with higher economic income especially need interventions and nutrition education to help them acquire nutrition knowledge for a healthy lifestyle. The positive impact of economic income on the BMI trajectories of the elderly provides further directions for preventing and controlling obesity in the elderly.

Nutrients doi: 10.3390/nu17010033

Authors: Dongxiang Huang Xiaobing Wang Hideki Takagi Shiwei Mo Zhongzheng Wang Daniel Hung-Kay Chow Bo Huang

Background: Nutritional supplements are widely used by swimmers, but the effectiveness of various supplements and the identification of the most effective intervention require further investigation. Purpose: This paper evaluated and compared the effectiveness of various nutrition-based interventions on swimming performance through both direct and indirect comparisons. Methods: PubMed, Embase, Web of Science, Cochrane Library, and SPORTDiscus databases were thoroughly searched up to 4 April 2024. The risk of bias was judged using the Cochrane risk of bias tool. A random-effect model was adopted to compute standardized mean differences (SMD) and 95% confidence intervals (CI). Results: L-arginine (Arg) demonstrated superior performance to the placebo (SMD = −1.66, 95% CI [−2.92, −0.44]), emerging as the most effective intervention for reducing 100 swimming time (SUCRA = 89.5%). Beta-alanine (BA) was the best intervention for improving blood lactate (SUCRA = 80%). Creatine combined with sodium bicarbonate (Creatine_NaHCO3) significantly increased blood pH compared to the placebo (SMD = 3.79, 95% CI [1.85, 5.80]), with a SUCRA score of 99.9%, suggesting it is the most effective intervention for this parameter. No prominent differences were noted among the interventions in 50 m time, 200 m time, heart rate, and body mass. Conclusions: Dietary supplements might provide benefits for improving swimming performance. Arg emerged as the most efficacious modality for reducing 100 m time. BA proved to be the preeminent strategy for decreasing blood lactate. Creatine_NaHCO3 was distinguished as the optimal approach for improving blood pH.

Nutrients doi: 10.3390/nu17010032

Authors: Alissa A. Nolden James Makame

Background/Objectives: Low- and no-calorie sweeteners (LNCSs) provide sweetness in food applications that are suggested to support consumers’ reduced consumption of caloric sweeteners and added sugar intake. Consumers seek guidance and advice on using LNCSs from healthcare providers, including dietitians and nutritionists. However, prior research suggests there may be inconsistent guidance on the use of LNCSs. The main goal is to assess dietitians’ and nutritionists’ perceptions and knowledge of LNCSs. We also evaluate the impact of educational intervention on the perceptions and knowledge of low- and no-calorie sweeteners (LNCSs) among healthcare professionals. Methods: Participants completed an online questionnaire on their perceptions of LNCSs before and after viewing a webinar given by a member of the FDA on the safety and approval process of LNCSs. A total of 187 participants completed the pre-questionnaire, and 58 participants completed the post-questionnaire. Results: The LNCSs most familiar to participants was aspartame, followed by stevia, with thaumatin, advantame, and neotame being the least familiar sweeteners. While all sweeteners were FDA-approved, there were differences in perceived safety and suitability for dietary use. Following the intervention, there was a significant improvement in the participant’s knowledge, understanding, and confidence in the safety and approval process of LNCSs and reduced negative perceptions of LNCSs on health. Conclusions: With nutritionists and dietitians being the primary sources of nutritional guidance, consumers may seek their recommendations regarding using LNCSs. However, this study revealed differences in suitability across FDA-approved LNCSs and a lack of confidence regarding the safety and approval process. Education programs regarding the safety and approval process of LNCSs increase confidence in advising patients and clients on the use of LNCSs.

Nutrients doi: 10.3390/nu17010031

Authors: Astrid Lounici Ana Iacob Katarzyna Hongler Melina A. Mölling Maria Drechsler Luca Hersberger Shebani Sethi Undine E. Lang Timur Liwinski

The substantial evidence supporting the ketogenic diet (KD) in epilepsy management has spurred research into its effects on other neurological and psychiatric conditions. Despite differences in characteristics, symptoms, and underlying mechanisms, these conditions share common pathways that the KD may influence. The KD reverses metabolic dysfunction. Moreover, it has been shown to support neuroprotection through mechanisms such as neuronal energy support, inflammation reduction, amelioration of oxidative stress, and reversing mitochondrial dysfunction. The adequate intake of dietary nutrients is essential for maintaining normal brain functions, and strong evidence supports the role of nutrition in the treatment and prevention of many psychiatric and neurological disorders. Obsessive–compulsive disorder (OCD) is a neuropsychiatric condition marked by persistent, distressing thoughts or impulses (obsessions) and repetitive behaviors performed in response to these obsessions (compulsions). Recent studies have increasingly examined the role of nutrition and metabolic disorders in OCD. This narrative review examines current evidence on the potential role of the KD in the treatment of OCD. We explore research on the KD’s effects on psychiatric disorders to assess its potential relevance for OCD treatment. Additionally, we identify key gaps in the preclinical and clinical research that warrant further study in applying the KD as a metabolic therapy for OCD.

Nutrients doi: 10.3390/nu17010030

Authors: Melese Sinaga Teshome Eugene Rameckers Sarah Mingels Marita Granitzer Teklu Gemechu Abessa Liesbeth Bruckers Tefera Belachew Evi Verbecque

Background: Malnutrition has extensive consequences, affecting multiple levels of functioning, including motor skill impairments. However, current interventions have mainly focused on dietary treatment, often neglecting motor impairments and relying solely on clinical and anthropometric indicators to assess treatment response. This study aims to bridge this gap by examining the combined effect of ready-to-use supplementary food (RUSF) and high-intensity motor learning (HiML) on motor skill-related physical fitness in children with moderate thinness (MT). Methods: A cluster randomized controlled trial was conducted among children 5–7 years old with MT in Jimma Town. Three schools were randomized to three intervention arms, including a total of 69 children: RUSF (n = 23), RUSF + HiML (n = 25), and no intervention (n = 21). The HiML training was applied for 12 weeks, and RUSF was distributed daily for 12 weeks. HiML was given daily (1 h/day, 5 days/week). The primary outcome was motor skill-related physical fitness assessed at baseline and endline using the performance and fitness test battery (PERF-FIT). The changes from baseline to endline measurements were calculated as differences, and the mean difference in these changes/differences (DID) was then computed as the outcome measure. AN(C)OVA was used to directly investigate differences between groups. Statistical significance was declared at p-value ≤ 0.05. Results: There was a significantly greater and comparable improvement in both the RUSF and RUSF + HiML groups compared to the control group for the ‘stepping’ item (p < 0.001), the ‘side jump’ item (p < 0.001), the ‘standing long jump’ (p < 0.001) and the ‘jumping and hopping’ total (p = 0.005). The RUSF + HiML group showed significantly greater improvements in the ‘bounce and catch’ (p = 0.001) and ‘throw and catch’ (p < 0.001) items compared to the RUSF group, which, in turn, demonstrated greater improvement than the control group in both items (p < 0.01). Conclusions: A 12-week combination of RUSF + HiML was proven to be safe in children with MT and caused clear improvements in motor skill-related physical fitness. When the children received RUSF with HiML training, similar gains in stepping, side jump, standing long jump, and jumping and hopping were observed, except for the ball skills where the HiML training group performed better.

Nutrients doi: 10.3390/nu17010029

Authors: Adriana Duelo Sònia Sánchez-Pérez Ana María Ruiz-Leon Francesc Casanovas-Garriga Salvador Pellicer-Roca Irache Iduriaga-Platero Judit Costa-Catala M. Teresa Veciana-Nogués Joaquim Fernández-Solà Rosa M. Muñoz-Cano Joan Bartra Andrea Combalia Oriol Comas-Basté Rosa Casas M. Luz Latorre-Moratalla Ramon Estruch M. Carmen Vidal-Carou

Background/Objectives: Histamine intolerance is primarily caused by a deficiency in the diamine oxidase (DAO) enzyme at the intestinal level. The reduced histamine degradation in the gut leads to its accumulation in plasma, thereby causing multiple clinical manifestations, such as urticaria, diarrhea, headache, dyspnea, or tachycardia, among others. The dietary management of this food intolerance consists of the follow-up of a low-histamine diet, often combined with DAO supplementation. To date, around twenty studies have investigated the effectiveness of these dietary strategies in reducing the frequency and/or intensity of symptoms, with promising results. However, the limitations of these studies (small patient cohort, lack of control group, and short dietary intervention periods) highlight the need for more ambitiously designed research. Therefore, the main objective of this prospective, unicentric, double-blind, randomized, and placebo-controlled trial is to evaluate the efficacy of a low-histamine diet and/or DAO supplementation over a three-month period in improving symptoms of histamine intolerance. Additionally, the impacts of these dietary strategies on the intestinal microbiota composition, urinary profile of histamine metabolites, serum DAO activity, and plasma histamine levels will be assessed throughout the intervention. Methods: The trial will enroll 400 patients who will be randomly assigned to one of two groups: the intervention group, which will follow a low-histamine diet, or the control group, which will maintain their habitual dietary habits. Within each of these groups, participants will be further divided into four subgroups to receive either exogenous DAO enzyme supplementation (from porcine or plant sources, with the latter administered at two different dosages) or a placebo. Therefore, a total of eight distinct intervention groups will be considered. The comparison of these groups will allow the evaluation of the individual effects of the low-histamine diet or DAO enzyme supplementation, as well as their possible synergistic effect. Results: The results of this study should help to improve dietary recommendations for histamine-intolerant patients and ultimately enhance their quality of life.

Nutrients doi: 10.3390/nu17010028

Authors: Sharfa Khaleel Tareq Osaili Dana N. Abdelrahim Falak Zeb Farah Naja Hadia Radwan MoezAlIslam E. Faris Hayder Hasan Leila Cheikh Ismail Reyad S. Obaid Mona Hashim Wael Ahamd Bani Odeh Khalid Abdulla Mohd Hajer Jassim Al Ali Richard A. Holley

Background/Objectives: The escalating global demand for meat, as a sequela of population growth, has led to unsustainable livestock production, resulting in a host of environmental and food security concerns. Various strategies have been explored to mitigate these issues, including the introduction of a novel food product, cultured meat. Cultured meat is not yet commercially available, yet public perceptions are already taking shape. To better understand the factors influencing its adoption by consumers, a cross-sectional, web-based study was conducted to examine consumer attitudes toward conventional meat and cultured meat among adults in the United Arab Emirates (UAE). Methods: The survey was conducted between December 2023 and March 2024 and used a convenience snowball sampling method. The questionnaire focused on current meat consumption patterns, meat attachment, and willingness to consume cultured meat. Sociodemographic data, including age, sex, education, and self-reported weight and height, were also collected. Results: Results showed that the vast majority (86%) of participants consumed all types of meats, while more than half (59.3%) were unfamiliar with the term “cultured meat”. Despite this unfamiliarity, about one-third (35%) were somewhat willing to try cultured meat, though more than two-thirds (69%) were reluctant to replace conventional meat with cultured meat in their diet. Male participants and those with higher BMIs showed a significantly stronger attachment to conventional meat. Willingness to consume cultured meat was notably higher among participants aged less than 30 years, those having less formal education, and those who are Arabic. Conclusions: These findings suggest that while interest in cultured meat exists, significant barriers remain, particularly regarding consumer education and cultural acceptance.

Nutrients doi: 10.3390/nu17010024

Authors: Dongho Shin Hyong Woo Moon Woong Jin Bae U-Syn Ha Young Ho Park Eun Joo Lee Du Geon Moon Sae Woong Kim

Background: The prevalence of urological diseases increases with age, and lower urinary tract symptoms (LUTSs) are the most common problem. Natural compounds with minimal side effects for the improvement in LUTSs are of ongoing interest. Salvia miltiorrhiza root extract (SAGX) has shown potential in preclinical studies for its effects on LUTSs. Objectives: This multicenter, randomized, double-blind, placebo-controlled study aimed to evaluate the efficacy and safety of SAGX in men with lower urinary tract symptoms (LUTSs) over a 12-week period. Methods: A total of 136 subjects were randomized to receive either 400 mg or 800 mg of SAGX or a placebo daily, orally. The primary outcome was the change in the International Prostate Symptom Score (IPSS). Secondary outcomes included changes in prostate-specific antigen (PSA), testosterone levels, urinary flow rate, residual urine volume, and erectile function as measured by the International Index of Erectile Function (IIEF). Results: Both SAGX intake groups showed statistically significant improvements in total IPSS scores and several secondary outcomes compared with the placebo group. Notable improvements were observed in symptoms of incomplete emptying, frequency, intermittency, weak stream, urgency, nocturia, and quality of life scores. Erectile function, as assessed by the IIEF, also significantly improved, especially in the 400 mg SAGX intake group. No significant differences were found in PSA levels or testosterone levels. No serious adverse events leading to discontinuation of the study drug were observed in the SAGX groups. Conclusions: With fewer side effects than conventional treatments, SAGX is effective and safe in improving symptoms of lower urinary tract symptoms and enhancing erectile function in men.

Nutrients doi: 10.3390/nu17010026

Authors: Alice Rosi Francesca Scazzina Francesca Giampieri Ludwig Álvarez-Córdova Osama Abdelkarim Achraf Ammar Mohamed Aly Evelyn Frias-Toral Juancho Pons Laura Vázquez-Araújo Carmen Lili Rodríguez Velasco Julién Brito Ballester Lorenzo Monasta Ana Mata Adrián Chacón Pablo Busó Giuseppe Grosso

Background/Objectives. Traditional dietary patterns are being abandoned in Mediterranean countries, especially among younger generations. This study aimed to investigate the potential lifestyle determinants that can increase adherence to the Mediterranean diet in children and adolescents. Methods. This study is a cross-sectional analysis of data from five Mediterranean countries (Italy, Spain, Portugal, Egypt, and Lebanon) within the context of the EU-funded project DELICIOUS (UnDErstanding consumer food choices & promotion of healthy and sustainable Mediterranean Diet and LIfestyle in Children and adolescents through behavIOUral change actionS). This study comprised information on 2011 children and adolescents aged 6–17 years old collected during 2023. The main background characteristics of both children and parents, including age, sex, education, and family situation, were collected. Children’s eating (i.e., breakfast, place of eating, etc.) and lifestyle habits (i.e., physical activity level, sleep, and screen time) were also investigated. The level of adherence to the Mediterranean diet was assessed using the KIDMED index. Logistic regression analyses were performed to test for likelihood of higher adherence to the Mediterranean diet. Results. Major determinants of higher adherence to the Mediterranean diet were younger age, higher physical activity level, adequate sleep duration, and, among dietary habits, having breakfast and eating with family members and at school. Parents’ younger age and higher education were also determinants of higher adherence. Multivariate adjusted analyses showed that an overall healthier lifestyle and parents’ education were the factors independently associated with higher adherence to the Mediterranean diet. Conclusions. Higher adherence to the Mediterranean diet in children and adolescents living in the Mediterranean area is part of an overall healthy lifestyle possibly depending on parents’ cultural background.

Nutrients doi: 10.3390/nu17010027

Authors: Karen Berenice Guzmán-Castellanos Itziar Zazpe Susana Santiago Maira Bes-Rastrollo Miguel Ángel Martínez-González

Background/Objectives: Noncommunicable diseases, particularly cardiovascular disease (CVD), represent a significant global public health challenge, with unhealthy diets as a major risk factor. This study investigates the association between adherence to the Planetary Health Diet proposed by the EAT-Lancet Commission and CVD risk. Methods: Utilizing data from the Seguimiento Universidad de Navarra (SUN) cohort, which included 18,656 participants (mean age 38 years; 61% women), we assessed dietary intake using a validated food frequency questionnaire and the Planetary Health Diet Index to evaluate adherence (range 0–42). CVD was defined as new-onset stroke, myocardial infarction, or CVD death. Results: After a median follow-up time of 11.5 years, 220 cases of CVD were identified. Higher adherence to the Planetary Health Diet revealed no statistically significant reduction in CVD risk associated with the diet. Cox proportional hazard models indicated a trend towards lower CVD risk in the highest adherence quartile, but this did not reach significance (HR 0.77, 95% CI 0.51–1.18, p-trend = 0.127). Sensitivity analyses corroborated these results. Discrepancies in previous studies highlight the complexity of dietary assessments and underscore the need for standardized scoring systems. Conclusions: In a large Spanish cohort, adherence to the Planetary Health Diet showed no significant reduction in CVD risk. Further research is needed to reach a consensus on the operational definition of the Planetary Health Diet and to clarify the relationship between diet and CVD risk.

Nutrients doi: 10.3390/nu17010025

Authors: Siyuan Liu Lun Hua Xi Mo Bing Lei Ruihao Zhang Shihao Zhou Xuemei Jiang Zhengfeng Fang Bin Feng Lianqiang Che Shengyu Xu Yan Lin De Wu Yong Zhuo Chao Jin

Background: Maternal obesity detrimentally affects placental function and fetal development. Both alternate-day fasting (ADF) and time-restricted feeding (TRF) are dietary interventions that can improve metabolic health, yet their comparative effects on placental function and fetal development remain unexplored. Objectives: This study aims to investigate the effects of ADF and TRF on placental function and fetal development during maternal consumption of a high-fat diet (HFD). Methods: One hundred 8-week-old female mice were assigned to one of four dietary regimens: (1) normal diet with ad libitum feeding (NA); (2) HFD with ad libitum feeding (HA); (3) HFD with ADF (HI); and (4) HFD with TRF (HT), administered both before and during pregnancy. On gestational day 18.5, serum and placental samples were collected from both mothers and fetuses to examine placental function and fetal development. Results: During gestation, both ADF and TRF substantially alleviated the metabolic impairments caused by an HFD in obese maternal mice. TRF mice demonstrated enhanced placental nutrient transport and fetal development, associated with reduced endoplasmic reticulum (ER) stress and inflammatory responses. In contrast, ADF markedly intensified placental stress and inflammatory responses, diminished placental nutrient transport efficiency, and consequently induced fetal growth restriction. Conclusions: Both ADF and TRF during pregnancy significantly mitigated metabolic impairments in obese dams on an HFD. TRF mice demonstrated enhanced placental nutrient transport and fetal development, associated with reduced endoplasmic reticulum (ER) stress and inflammatory responses. In contrast, ADF markedly intensified placental stress and inflammatory responses, diminished placental nutrient transport efficiency, and consequently induced fetal growth restriction.

Nutrients doi: 10.3390/nu17010023

Authors: Giulia Camporesi Alessandra Bordoni

Background/Objectives: The water footprint (WF) provides information on the impact of individual foods on water consumption, but to better direct food production toward water saving, we need to understand how to reduce the WF of our diets while keeping it healthy. In this study, we compared the WF of healthy diets based on national food-based dietary guidelines with the aim of highlighting changes in dietary patterns that could reduce water requirements without compromising nutritional adequacy. Methods: Three 2000 kcal/day dietary patterns were elaborated following the Italian, Spanish, and American dietary guidelines, and their total, green, blue, and grey WFs were calculated. Results: The Italian dietary pattern showed the lowest total WF (2806 L per capita/day), with the American and Spanish patterns being 8% and 10.5% higher, respectively. The food groups contributed differently to the total WF. In the USA, animal foods were the main contributor (56% versus 41% in Spain and 38% in Italy). The contribution of plant foods was higher in Italy (61%) than in Spain (54%) and the USA (38%). The distribution of the total WF between WFgreen, WFblue, and WFgrey was similar across the dietary patterns. Within each food group, and mainly in the animal-origin food group, the type of product significantly modulated the WF. Conclusions: Different diets can be equally nutritionally sustainable but have different impacts on environmental sustainability. The comparison of their WFs can be the starting point to promote dialogue between nutritionists, operators in the environmental sector, and the agri-food industry to ensure a healthy and balanced approach.

Nutrients doi: 10.3390/nu17010022

Authors: Erika Barreto Aléssio Almeida Adélia Neta Flávia Ferreira

Background/Objectives: The study prospectively assessed the association between sleep duration and the consumption of ultra-processed foods in adolescent students from a Brazilian capital. Methods: We observed 1431 adolescents participating in the Longitudinal Study on sedentary behavior, physical activity, dietary habits, and adolescent health, aged 10 to 14 years, in the years 2014 to 2017. The percentage of consumption of ultra-processed foods was evaluated by the Friedman test. Generalized Estimation Equation (GEE) models with parameters for robust analysis were used to verify the prospective association between sleep and the consumption of ultra-processed foods. Results: The percentage consumption of ultra-processed foods in relation to total daily calories increased in boys between 15 and 17 years old (31.8% in 2015 to 35.8% in 2017), being more stable among older girls (36.7% in 2015 to 37.7% in 2017), which may have occurred due to the influence of the social environment, reduced critical capacity, and lower concern for nutrition. This excessive consumption can lead to health and body composition impairments. Over time, adolescents who were in the fourth quartile of sleep had a 1.4% reduction (95% CI −2.49; −0.28) in the energy percentage from ultra-processed foods. When adjusting the initially used model in the GEE (Generalized Estimation Equation) with sociodemographic variables (income, maternal education, age) and lifestyle factors, sleep duration decreased by 2.1% (95% CI −4.09; −0.22) only in boys. The shorter duration of sleep in adolescents has been shown to be associated with unhealthy behavioral factors, such as a preference for fatty and sugary foods. There were no statistically significant associations for girls in this model. Conclusions: Sleep influences the consumption of ultra-processed foods in adolescents, showing that as sleep time increases, the consumption of foods classified as ultra-processed decreases.

Nutrients doi: 10.3390/nu17010021

Authors: Elena Bozzola Flavia Cirillo Cristina Mascolo Livia Antilici Umberto Raucci Benedetta Guarnieri Annamaria Ventricelli Elettra De Santis Giulia Spina Massimiliano Raponi Alberto Villani Maria Rosaria Marchili

Background: Anorexia nervosa is a serious eating disorder that mainly affects children and adolescents. Most patients present with extreme body dissatisfaction and an obsessive focus on body weight and food. Anorexia nervosa is a complex and multifactorial condition characterised by biological, psychological, and social factors. However, studies that have explored the cumulative risk that predisposes to anorexia nervosa are limited. This study aims to explore the potential risk factors for a severe form of the disease in patients affected by anorexia nervosa and to identify whether they may interact and reinforce each other, contributing to the severity of the disorder. Methods: For this study, we enrolled children and adolescents under 18 years of age hospitalised at IRCCS Bambino Gesù Pediatric Hospital, Rome, Italy, for anorexia nervosa from 1 December 2022 to 31 August 2024, identifying and analysing potential risk factors. Elevated shape and weight concerns were found in all patients. Psychiatric and neurodevelopment comorbidities were identified in 76 patients (51.35%), life stress events in 69 (46.62%), and a family history of eating and weight control behaviours in 39 (26.35%). Out of the sample size, 20.27% of patients did not live in a traditionally structured family. This study used the Kiddie-SADS-Present and Lifetime Version interview, the Coddington Life Events Scales, and the Trauma Symptom Checklist for Children questionnaires. Results: Patients with an extreme or severe index of anorexia nervosa are more likely to have multiple predisposing factors. In detail, four predisposing factors were found in 18.6% of patients with an extreme severity index, in 15.5% of those with a severe score, and in 10.3 and 10.6% of those with a moderate and mild score, respectively. Conclusions: Cumulative potential risk factors are more likely to be found in cases of severe course disease and patients hospitalised for anorexia. Prompt identification of predisposing factors and an effective plan of action are required to avoid a severe course disorder.

Nutrients doi: 10.3390/nu17010020

Authors: Rassul Kuatov Jiro Takano Hideyuki Arie Masaru Kominami Norifumi Tateishi Ken-ichi Wakabayashi Daisuke Takemoto Takayuki Izumo Yoshihiro Nakao Wataru Nakamura Kazuyuki Shinohara Yasukazu Nakahata

Background/Objectives: Circadian clocks are endogenous systems that regulate numerous biological, physiological, and behavioral events in living organisms. Aging attenuates the precision and robustness of circadian clocks, leading to prolonged and dampened circadian gene oscillation rhythms and amplitudes. This study investigated the effects of food-derived polyphenols such as ellagic acid and its metabolites (urolithin A, B, and C) on the aging clock at the cellular level using senescent human fibroblast cells, TIG-3 cells. Methods: Lentivirus-infected TIG-3 cells expressing Bmal1-luciferase were used for real-time luciferase monitoring assays. Results: We revealed that urolithins boosted the amplitude of circadian gene oscillations at different potentials; urolithin A (UA) amplified the best. Furthermore, we discovered that UA unstabilizes PER2 protein while stabilizing SIRT1 protein, which provably enhances BMAL1 oscillation. Conclusions: The findings suggest that urolithins, particularly UA, have the potential to modulate the aging clock and may serve as therapeutic nutraceuticals for age-related disorders associated with circadian dysfunction.

Nutrients doi: 10.3390/nu17010019

Authors: Po-Wen Chen

The meta-analysis on probiotics’ effects on cortisol levels demonstrates their potential to modulate stress responses across diverse populations, though variability in strain efficacy highlights the need for standardization and _targeted studies [...]

Nutrients doi: 10.3390/nu17010015

Authors: Stephen Inns Heidi Su Amanda Chen Crispin Ovenden Joy Alcantara Priyanka Lilic Helen Myint

Background: Body image dissatisfaction is elevated in inflammatory bowel disease (IBD) as well as other chronic diseases. The aim of this study was to determine if the higher rate of body image dissatisfaction in IBD is specific to IBD or characteristic of chronic disease in general by comparing body image dissatisfaction in IBD patients with age- and gender-matched healthy individuals and those with type 1 diabetes mellitus (T1DM). Methods: In this New Zealand-based case-control study conducted in a secondary care hospital, consecutive IBD patients aged 16 years and older were matched 1:1 with healthy individuals and T1DM patients based on age and gender. However, availability of controls resulted in a slightly different number of pairs for each comparison between groups. Demographics were documented, and participants completed the Body Image Disturbance Questionnaire (BIDQ), RAND SF-36 Quality of Life measures, and Hospital Anxiety and Depression Scale (HADS). Results: Forty-five matched pairs compared IBD patients to healthy controls, while 38 compared IBD patients to T1DM patients. BIDQ scores were higher for IBD patients than healthy controls (2.05 vs. 1.58, p = 0.004) but not significantly different from T1DM patients (2.03 vs. 1.72, p = 0.09). No differences were seen in BMI, smoking, or relationship status across groups. IBD patients had higher depression scores than controls (mean 6.51 vs. 3.87, p = 0.002) but similar anxiety scores (5.51 vs. 4.89, p = 0.258). A 1-point BIDQ increase in IBD patients was associated with a 4.6-fold increase in depression (p = 0.025), after adjusting for clinical and demographic factors. Conclusions: Body image dissatisfaction is prevalent in IBD patients and may be a common feature across chronic diseases. Body image dissatisfaction strongly associates with depression, highlighting the importance of addressing it in IBD management.

Nutrients doi: 10.3390/nu17010018

Authors: Agnieszka Kolmaga Elżbieta Trafalska Ewelina Gaszyńska Anna Gawron-Skarbek Sławomir Witkowski Julia Murlewska Maria Respondek-Liberska Iwona Strzelecka

Introduction: The relationship between diet of mothers, including supplementation of vitamin D and Long Chain Polyunsaturated Fatty Acids (LC-PUFA), and the prevalence of congenital heart defects (CHD) in the fetus has not been sufficiently studied. The aim of the study was to investigate the relationship between the intake of vitamin D and LC-PUFA by mother (from diet and with supplementation, including its time of implementation and applied dose), and the risk of CHD in the fetus. Methods: This was a case—control study with the participation of a total of 79 women with prenatally diagnosed CHD in the fetus and 121 women without CHD in the fetus. The Food Frequency Questionnaire (FFQ) was used with particular emphasis on vitamin D and DHA supplementation. A univariate logistic regression model was used to evaluate the relationship between selected dietary data and the risk of CHD in the fetus. Results: In the group of females without CHD in the fetus, the mean values of vitamin D intake, including supplementation, and dietary LC-PUFA + DHA from supplementation, were higher than in women with CHD in the fetus (41.3 ± 24.7 vs. 34.7 ± 27.8 μg; p = 0.02 and 831.0 ± 280.1 vs. 767.7 ± 287.6 mg; p = 0.008, for vitamin D and LC-PUFA + DHA respectively). No significant relationship was found between vitamin D intake (including supplementation) or between LC-PUFA + DHA supplementation by pregnant women, and the presence of CHD in their children. Conclusions: It seems that increased maternal intake of vitamin D and LC-PUFA, including DHA, through supplementation, may protect fetus against CHD, but the relationship between the occurrence of CHD and diet in this area need further studies.

Nutrients doi: 10.3390/nu17010017

Authors: Hiroshi Kusunoki Yoko Hasegawa Yasuyuki Nagasawa Kensaku Shojima Hiromitsu Yamazaki Takara Mori Shotaro Tsuji Yosuke Wada Kayoko Tamaki Koutatsu Nagai Ryota Matsuzawa Hiromitsu Kishimoto Hideo Shimizu Ken Shinmura

Background/Objectives: Oral frailty, first identified in Japan in 2014, refers to a state between healthy oral function and severe decline, marked by minor issues, such as tooth loss and chewing difficulties. The oral frailty five-item checklist (OF-5) enables non-dental professionals to evaluate oral frailty using five key indicators: remaining teeth count, chewing difficulties, swallowing difficulties, dry mouth, and articulatory oral skills. Limited studies exist. Methods: This study examined the relationship between oral and physical frailties in older adults and assessed the prognosis of physical frailty using the OF-5. Participants aged ≥65 years were recruited from the frail elderly in the Sasayama–Tamba area, Hyogo, Japan, and their physical function was assessed in terms of grip strength, walking speed, and skeletal muscle mass. Blood markers, such as cystatin C, an indicator of renal function, were also analyzed. Results: A cross-sectional analysis indicated that oral frailty was correlated with reduced muscle mass, walking speed, and physical function. Women had lower hemoglobin and albumin levels and a greater prevalence of frailty than men. Longitudinal analysis revealed that initial OF-5 scores predicted increased physical frailty after 2–3 years, especially in those with higher baseline scores. The OF-5 was a significant factor for frailty progression in both sexes. Conclusions: These results suggest that early detection of oral frailty via the OF-5 may be useful in preventing the progression of overall frailty in older adults.

Nutrients doi: 10.3390/nu17010016

Authors: Paulina Kleniewska Rafał Pawliczak

Background: Asthma (a chronic inflammatory disease of the airways) is characterized by a variable course, response to treatment, and prognosis. Its incidence has increased significantly in recent decades. Unfortunately, modern lifestyle and environmental factors contribute to the further increase in the incidence of this disease. Progressive industrialization and urbanization, widespread use of antibiotic therapy, excessive sterility and inappropriate, highly processed diets are some of the many risk factors that are relevant today. Over the years, a lot of evidence has been gathered showing the influence of microorganisms of the gut or airways on human health. Studies published in recent years indicate that dysbiosis (microbial imbalance) and oxidative stress (pro-oxidant–antioxidant imbalance) are important elements of the pathogenesis of this inflammatory disease. Scientists have attempted to counteract the effects of this process by using probiotics, prebiotics, and antioxidants. The use of probiotic microorganisms positively modulates the immune system by maintaining homeostasis between individual fractions of immune system cells. Moreover, recently conducted experiments have shown that probiotics have antioxidant, anti-inflammatory, and protective properties in oxidative stress (OS). The aim of this study is to present the current state of knowledge on the role of dysbiosis and OS in the pathogenesis of asthma. Conclusions: This review highlights the importance of using probiotics, prebiotics, and antioxidants as potential strategies to support the treatment and prevention of this disease.

Nutrients doi: 10.3390/nu17010014

Authors: Giuseppe Derosa Angela D’Angelo Fabrizio Angelini Luca Belli Arrigo F. G. Cicero Roberto Da Ros Giovanni De Pergola Giovanni V. Gaudio Alessandro Lupi Giovanni Sartore Federico A. Vignati Pamela Maffioli

Dysglycemia is a condition preceding diabetes mellitus. The two situations inherent in this condition are called impaired fasting glucose (IFG) and impaired glucose tolerance (IGT). If one of these situations is found in the patient, after the advice of an appropriate diet and physical activity, the addition of nutraceuticals or supplements can be considered, which can stop or delay the progression to diabetes mellitus over time. The purpose was to compile a systematic review about the use of nutraceuticals for treating diabetes and prediabetes and to offer a valuable resource for colleagues working on this crucial subject, thereby improving patient health. The added value of the paper compared to other reviews is that it was written by experts appointed by five different scientific societies dealing with diabetes, nutrition, and complications.

Nutrients doi: 10.3390/nu17010013

Authors: Sonia Spinelli Zelle Humma Mirko Magnone Elena Zocchi Laura Sturla

Abscisic acid (ABA) is a hormone with a long evolutionary history, dating back to the earliest living organisms, of which modern (ABA-producing) cyanobacteria are likely descendants, which existed long before the separation of the plant and animal kingdoms, with a conserved role as signals regulating cell responses to environmental challenges. In mammals, along with the anti-inflammatory and neuroprotective function of ABA, nanomolar ABA regulates the metabolic response to glucose availability by stimulating glucose uptake in skeletal muscle and adipose tissue via an insulin-independent mechanism and increasing metabolic energy production and also dissipation in brown and white adipocytes. Chronic ABA intake of micrograms per Kg body weight improves blood glucose, lipids, and morphometric parameters (waist circumference and body mass index) in borderline subjects for prediabetes and metabolic syndrome. This review summarizes the most recent in vitro and in vivo data obtained with nanomolar ABA, the involvement of the receptors LANCL1 and LANCL2 in the hormone’s action, and the importance of mammals’ endowment with two distinct hormones governing the metabolic response to glucose availability. Finally, unresolved issues and future directions for the clinical use of ABA in diabetes are discussed.

Nutrients doi: 10.3390/nu17010012

Authors: Calin Avram Victoria Nyulas Danusia Onisor Ion Mihai Georgescu Julianna Szakacs Florina Ruta

Introduction: A key element for a healthy lifestyle in the formative years of a young adult is a balanced, healthy, individualized diet. Aim: The aim of this study was to analyze the eating behavior and lifestyles of students at a university in Romania, as well as the extent to which the study program interferes with these factors. Methods: A retrospective study was performed, with the data being collected through an online questionnaire via the Google Forms platform; data collection for the current study was carried out in November 2024 during a student session. Results: Out of the total of 501 questionnaires collected, 416 were completed by women and 85 by men. It can be observed that 57.65% of men (49 participants) eat three meals a day compared to 53.13% of women (221 participants). The university program influenced the students’ meal schedules for 59.88% (n = 300) of participants, while 30.94% (n = 155) declared that they never have an ordered meal schedule. For 39.92% of students, the exam period has an influence on the meal schedule, including an increase the intake of sweet foods (59.88%), and some declared that they forget to eat (33.93%). Conclusions: The food and lifestyle behaviors in the studied group show health risk elements, especially an increase in tobacco consumption, a lack of regular meal schedules, and the consumption of an excess of unhealthy snacks. It was found that intensive study schedules may intensify this behavior.

Nutrients doi: 10.3390/nu17010011

Authors: Noèlia Carrasco-Querol Lorena Cabricano-Canga Nerea Bueno Hernández Carme Martín-Borràs Alessandra Queiroga Gonçalves Anna Vila-Martí Blanca Ribot Judit Solà Carme Valls-Llobet Rosa Caballol Angelats Pilar Montesó-Curto Elisabet Castro Blanco Macarena Pozo Ariza Sandra Carreres Rey Laura Pla Pagà Mònica Dearos Sanchís José Fernández-Sáez M. Rosa Dalmau Llorca Carina Aguilar Martín

Background: Multidisciplinary lifestyle interventions are being researched to treat fibromyalgia. However, the impact of nutrition as a key treatment component is little studied. This study aimed to evaluate the effectiveness of the SYNCHRONIZE + lifestyle multidisciplinary intervention in improving adherence to the Mediterranean diet, nutrition quality and dietary intake pattern in persons with fibromyalgia and chronic fatigue syndrome. Methods: A pragmatic randomized clinical trial was conducted in primary care. Data were collected using the 17-item energy-restricted Mediterranean Adherence Screener (er-MEDAS), the food frequency questionnaire (sFFQ) and the 24 h recall questionnaire (24 HR), in addition to chrono-nutritional, anthropometric, and body composition data, at baseline and 3-, 6-, and 12- month follow-up visits, and statistically analyzed. Results: A total of 158 participants were evaluated. Results showed the effectiveness of the intervention in improving adherence to the Mediterranean diet. The adherence depended on the group-time interaction being positive and significant at 3 and 6 months post-intervention in the INT group and on the participant age and educational level. Specifically, the intake of legumes, fruits, vegetables, nuts and blue fish was increased, while the intake of sweets and pastries, butter and cream and red and processed meat was reduced. Furthermore, the intake of chips and candies was also reduced, and the consumption of fermented food (yogurts, cheese, kefir) increased. Thus, general diet quality improved. Interestingly, the intake of key nutrients such as protein and iron increased. Furthermore, the number of night eaters was decreased significantly. Muscle mass index was also improved in the intervention group. These results were maintained in the medium to long term. Conclusion: SYNCHRONIZE + is a brief, low-cost, multidisciplinary intervention effective in improving adherence to the Mediterranean diet and improving nutritional and dietary intake patterns in persons with fibromyalgia and chronic fatigue syndrome. Further evaluation of the effect on quality of life and symptoms is needed.

Nutrients doi: 10.3390/nu17010010

Authors: Seung Min Baik Mina Kim Jae Gil Lee

Background: Nutritional support is crucial in critically ill patients to enhance recovery, reduce infections, and improve outcomes. This meta-analysis compared early enteral nutrition (EEN) and early parenteral nutrition (EPN) to evaluate their efficacy in adult critically ill patients. Methods: A systematic review of 14 studies involving 7618 patients was conducted, including randomized controlled trials, prospective cohorts, and retrospective analyses. The primary outcomes were mortality and infectious complications, while secondary outcomes included intensive care unit length of stay (ICU-LOS), hospital length of stay (H-LOS), mechanical ventilation days, and gastrointestinal (GI) complications. Results: The results showed no significant difference in mortality between EEN and EPN (OR 1.03, 95% CI 0.93–1.14). EEN reduced bloodstream infections (OR 0.73, 95% CI 0.57–0.93), ICU-LOS (MD −0.18 days, 95% CI −0.33 to −0.04), and H-LOS (MD −1.15 days, 95% CI −1.38 to −0.93). However, EEN was associated with higher GI complications, such as vomiting and diarrhea (OR 2.25, 95% CI 1.97–2.58), while mechanical ventilation days showed no significant difference. Conclusions: These findings support prioritizing EEN in critically ill patients with functional gastrointestinal systems to improve infection control and recovery while emphasizing the importance of careful monitoring to mitigate gastrointestinal complications.

Nutrients doi: 10.3390/nu17010009

Authors: Milena Alicja Stachelska Piotr Karpiński Bartosz Kruszewski

Background/Objectives: There is scattered information in the scientific literature regarding the characterization of probiotic bacteria found in fermented milk beverages and the beneficial effects of probiotic bacteria on human health. Our objective was to gather the available information on the use of probiotic bacteria in the prevention of civilization diseases, with a special focus on the prevention of obesity, diabetes, and cancer. Methods: We carried out a literature review including the following keywords, either individually or collectively: lactic acid bacteria; probiotic bacteria; obesity; lactose intolerance; diabetes; cancer protection; civilization diseases; intestinal microbiota; intestinal pathogens. Results: This review summarizes the current state of knowledge on the use of probiotic bacteria in the prevention of civilization diseases. Probiotic bacteria are a set of living microorganisms that, when administered in adequate amounts, exert a beneficial effect on the health of the host and allow for the renewal of the correct quantitative and qualitative composition of the microbiota. Probiotic bacteria favorably modify the composition of the intestinal microbiota, inhibit the development of intestinal pathogens, prevent constipation, strengthen the immune system, and reduce symptoms of lactose intolerance. As fermented milk beverages are an excellent source of probiotic bacteria, their regular consumption can be a strong point in the prevention of various types of civilization diseases. Conclusions: The presence of lactic acid bacteria, including probiotic bacteria in fermented milk beverages, reduces the incidence of obesity and diabetes and serves as a tool in the prevention of cancer diseases.

Nutrients doi: 10.3390/nu17010008

Authors: Kevin M. R. Nijssen Marco A. Chavez-Alfaro Peter J. Joris Jogchum Plat Ronald P. Mensink

Background: Recently, we reported that longer-term mixed nut intake significantly reduced serum total and low-density lipoprotein (LDL)-cholesterol, but these markers may not fully capture lipoprotein-related cardiovascular disease (CVD) risk. Objectives: This randomized, controlled, single-blinded, crossover trial in older adults with overweight or obesity examined the effects of longer-term mixed nut consumption on lipoprotein particle size, number, and lipid distribution. Methods: Twenty-eight participants (aged 65 ± 3 years; BMI 27.9 ± 2.3 kg/m2) completed two 16-week periods (control [no nuts] vs. mixed nuts (60 g/day: 15 g of walnuts, pistachios, cashews, and hazelnuts), separated by an 8-week washout. Plasma lipoprotein particle numbers, sizes, and lipid distributions across subclasses were analyzed using high-throughput nuclear magnetic resonance (NMR) spectroscopy. Results: Mixed nut consumption significantly reduced Apolipoprotein B (ApoB) concentrations (−0.07 g/L; p = 0.009), total cholesterol (−0.27 mmol/L; p = 0.047), non-HDL cholesterol (−0.28 mmol/L; p = 0.022), and total triacylglycerol (TAG) (−0.27 mmol/L; p = 0.008). Total very large-density lipoprotein (VLDL) particle numbers decreased by 24 nmol/L (p < 0.001), with reductions observed across all VLDL subclasses. Total LDL particle numbers (p = 0.044), specifically intermediate-density lipoprotein (IDL) (p = 0.002) and large LDL particles (p = 0.015), were also reduced, while HDL particle numbers and sizes were unaffected. The mixed nut intervention significantly reduced cholesterol concentrations across all VLDL subclasses and IDL (all p < 0.01), with no changes in LDL or HDL subclasses. TAG concentrations showed reductions across all lipoprotein subclasses (all p < 0.05). Conclusions: Longer-term mixed nut consumption may lower CVD risk in older adults and favorable shifts in apoB-containing lipoprotein subclasses towards a less atherogenic profile.

Nutrients doi: 10.3390/nu17010007

Authors: Sohyun Jeon Heaji Lee Sun-Yeou Kim Choong-Hwan Lee Yunsook Lim

Background/Objectives: Type 2 diabetes mellitus (T2DM) is considered a serious risk to public health since its prevalence is rapidly increasing worldwide despite numerous therapeutics. Insulin resistance in T2DM contributes to chronic inflammation and other metabolic abnormalities that generate fat accumulation in the liver, eventually leading to the progression of metabolic dysfunction-associated fatty liver disease (MAFLD). Recently, the possibility that microbial-derived metabolites may alleviate MAFLD through enterohepatic circulation has emerged, but the underlying mechanism remains unclear. In this research, we utilized metabolites obtained from the fermentation of guava leaf extract, which is well-known for its antidiabetic activity, to investigate their effects and mechanisms on MAFLD. Methods: Diabetes was induced by a high-fat diet and streptozotocin injection (80 mg/kg body weight) twice in mice. Subsequently, mice whose fasting blood glucose levels were measured higher than 300 mg/dL were administered with metabolites of Limosilactobacillus fermentum (LF) (50 mg/kg/day) or guava leaf extract fermented by L. fermentum (GFL) (50 mg/kg/day) by gavage for 15 weeks. Results: GFL supplementation mitigated hyperglycemia and hepatic insulin resistance. Moreover, GFL regulated abnormal hepatic histological changes and lipid profiles in diabetic mice. Furthermore, GFL enhanced energy metabolism by activating the sirtuin1 (SIRT1)/proliferator-activated receptor γ coactivator 1α (PGC1α)/peroxisome proliferator-activated receptor (PPAR)-α pathway in diabetic mice. Meanwhile, GFL supplementation suppressed hepatic inflammation in diabetic mice. Conclusions: Taken together, the current study elucidated that GFL could be a potential therapeutic to ameliorate hyperglycemia and hepatic steatosis by improving SIRT1/PGC-1α/ PPAR-α-related energy metabolism in T2DM.

Nutrients doi: 10.3390/nu17010006

Authors: Raquel Oliveira Bruno Cabrita Ângela Cunha Sónia Silva João P. M. Lima Diana Martins Fernando Mendes

Background: Lung cancer (LC) patients are prone to suffer from malnutrition. Malnutrition negatively affects patients’ response to therapy, increases the incidence of treatment-related side effects, and decreases survival. Early identification of LC patients who are malnourished or at risk of malnutrition can promote recovery and improve prognosis. Objective: This study aimed to assess the risk and nutritional status of lung cancer patients who are hospitalised, as well as to evaluate the impact of nutritional intervention on the risk of malnutrition. Methods: From January 2022 to December 2023, 53 LC patients hospitalised in a pulmonology department had their nutritional risk (initial and final) and nutritional status (initial) assessed. All were selected for nutritional intervention. Nutrition counselling was the first intervention option, along with dietary changes with/without oral nutritional supplements. Results: At the time of hospitalisation, 90.6% of the patients were at nutritional risk, 45.3% were classified as moderately malnourished, and 35.8% were classified as severely underweight. After the hospitalisation, 73.6% were at nutritional risk at the time of discharge, suggesting a statistically significant decrease in the number of patients with nutritional risk. Conclusions: Most LC patients hospitalised presented an altered nutritional status. Our study suggests that a nutritional intervention must be implemented to reduce malnutrition risk, which may impact prognosis. The comprehensive nutritional problems experienced by LC patients require nutritional assessment and improved individually tailored nutritional support.

Nutrients doi: 10.3390/nu17010005

Authors: Anca Elena But Raluca Maria Pop Georg Friedrich Binsfeld Floricuța Ranga Meda Sandra Orăsan Andra Diana Cecan Iulia Ioana Morar Elisabeta Ioana Chera Teodora Irina Bonci Lia Oxana Usatiuc Mădălina Țicolea Florinela Adriana Cătoi Alina Elena Pârvu Mircea Constantin Dinu Ghergie

Background: Inflammation-induced oxidative stress is a pathophysiological mechanism of inflammatory diseases. Treatments _targeting oxidative stress can reduce inflammatory tissue damage. Objectives: This study aimed to conduct phytochemical analysis and evaluate the antioxidant effects of the hydroalcoholic extract of Matricaria recutita blossoms (M. recutita) and Zingiber officinale rhizomes (Z. officinale). Materials and Methods: The phytochemical analysis was carried out by measuring the total polyphenol content, total flavonoid content, and polyphenolic compounds’ HPLC-ESI MS. The antioxidant activity was evaluated in vitro through H2O2 DPPH, FRAP, and NO scavenging assays. An in vivo experiment was performed on rats with turpentine oil-induced acute inflammation. Treatments were administrated orally for 10 days, with three dilutions of each extract (100%, 50%, 25%), and compared to the CONTROL, inflammation, Diclofenac, and Trolox groups. In vivo, the antioxidant activity was evaluated by measuring the total antioxidant capacity (TAC), total oxidative status (TOS), oxidative stress index (OSI), malondialdehyde (MDA), nitric oxide (NO), advanced oxidation protein products (AOPP), and total thiols (SH). Results: The phytochemical analysis found a high content of phenolic compounds in both extracts, and the in vitro antioxidant activity was significant. In vivo, M. recutita and Z. officinale extracts proved to be effective in increasing TAC and lowering oxidative stress markers, respectively, the TOS, OSI, MDA, and NO levels. The effects were dose-dependent, with the lower concentrations being more efficient antioxidants. Matricaria recutita and Z. officinale extract effects were as good as those of trolox and diclofenac. Conclusions: Treatment with M. recutita and Z. officinale alleviated inflammation-induced oxidative stress. These findings suggest that M. recutita and Z. officinale extracts could be a promising adjuvant antioxidant therapy in inflammatory diseases.

Nutrients doi: 10.3390/nu17010004

Authors: Shukun Liang Jintao Sun Xinshu Gu Ya Zhao Xiumin Wang Hui Tao Zhenlong Wang Yougang Zhong Jinquan Wang Bing Han

Objectives: The purpose of this study was to find the potential mechanism of two Lactobacillus (Lactobacillus plantarum L11 and Lactobacillus reuteri LR) on ameliorating obesity, including lipid metabolism and gut microbiota. The two isolates have been studied to have good characterization in vitro, but in vivo studies in modulating lipid metabolism and gut microbiota were not studied. Methods: In this study, mice with HFD supplemented with L11 or LR exhibited slower obesity progression, including reduced weight gain, abdominal fat accumulation, liver damage, inflammation, and adipose lesions. Results: Total cholesterol (TC) and triglycerides (TG) in the serum were significantly reduced (p < 0.01). The inflammatory marker interleukin-6 (IL-6) notably decreased (p < 0.05). Both Lactobacillus strains altered the gut microbiota composition, increasing the relative abundance of Alistipes and Lactobacillus, while L11 also raised Lachnospiraceae abundance. Results of the Western blot analysis showed that L11 and LR influenced the PPAR and AMPK pathways. Conclusions: L11 and LR can effectively reduce obesity by modulating gut microbiota and activating the PPAR–AMPK pathway, leading to decreased liver injury and systemic inflammation in mice fed with an HFD. In the future, the two probiotics may provide a new way for clinically ameliorating obesity on human beings.

Nutrients doi: 10.3390/nu17010003

Authors: Jean L. Fry Kayla R. Anderson

Establishing relationships between diet and human health is an active and critical area of research, and this Special Issue, “Association Between Nutrition, Diet Quality, Dietary Patterns, and Human Health and Diseases”, is a collection of research highlighting classic and emerging themes in this area [...]

Nutrients doi: 10.3390/nu17010002

Authors: Antonio Bevilacqua Barbara Speranza Angela Racioppo Antonella Santillo Marzia Albenzio Antonio Derossi Rossella Caporizzi Matteo Francavilla Deborah Racca Zina Flagella Michele Andrea De Santis Antonio Elia Giulia Conversa Luciana Luchetti Milena Sinigaglia Maria Rosaria Corbo

The gut microbiota plays a key role in health and disease, but it could be affected by various factors (diet, lifestyle, environment, genetics, etc.). Focusing on diet, while the role of the different styles and choices (Mediterranean vs. Western diet, vegan or vegetarian diets) has been extensively studied, there are a few comprehensive papers on the effects of additives and food processing. Therefore, the main goal of this manuscript is to propose an overview of the link between ultra-processed foods and the gut microbiota based on papers and data available in the literature. The literature search was performed on PubMed and Clinicaltrials.gov, and after the selection of the most relevant articles, the paper proposes a synopsis of the effects of some classes of additives (sweeteners, preservatives, emulsifiers, glutamate, etc.), as well as of some treatments, on the gut microbiota and some pathological conditions.

Nutrients doi: 10.3390/nu17010001

Authors: Giovanna D’Abbronzo Cinzia Quaglia Giuseppe Di Costanzo Roberta Testa Rosalba Giacco Gabriele Riccardi Olga Vaccaro Marilena Vitale

Background/Objectives: Despite the accumulating evidence on the detrimental impact of UPFs on health, a common limit of the available studies concerns the instruments used to collect information about the consumption of processed foods. Recently, a specific NOVA-FFQ was proposed for the evaluation of ultra-processed food (UPF) consumption, but it does not allow the simultaneous assessment of energy and nutrient intake. We evaluate the concordance between the NOVA-FFQ and a common questionnaire (EPIC-FFQ) when assessing (1) the intake of foods with different degrees of processing and (2) the relationship between diet composition and cardiometabolic profile. Methods: One hundred and thirty people with diabetes (70 men and 60 women) completed the NOVA-FFQ and the EPIC-FFQ in random order two weeks apart. Anthropometric and major cardiovascular risk factors were measured. Results: Non-significant differences were detected for processed culinary ingredients and processed foods; larger significant differences were observed for minimally processed foods and UPFs, which were somewhat underestimated by the EPIC-FFQ (−24% vs. −21%, respectively; p < 0.001). However, Bland–Altman plots showed intraindividual differences between the two questionnaires within an acceptable range, and the intraclass correlation showed a moderate consistency. Furthermore, the energy and nutrient composition of the diet and the metabolic parameters were comparable for people classified in the highest tertile of UPF consumption by either method. Conclusions: The NOVA-FFQ provides more detailed information on the consumption of UPF foods; however, the EPIC-FFQ is a valid alternative, particularly practical when the simultaneous assessment of the overall quality of the diet is needed.

Nutrients doi: 10.3390/nu16244426

Authors: Hilmi S. Rathomi Judith Katzenellenbogen Nahal Mavaddat Kirsty Woods Sandra C. Thompson

Background: Time-restricted eating (TRE) shows promise for managing weight and metabolic issues, yet its application in real-world healthcare settings remains underexplored. This study aims to assess the real-world utilisation and short-term outcomes of TRE in clinical practice. Methods: This observational study used a retrospective chart review of 271 adults who attended a metabolic specialist clinic between 2019 and 2023. Descriptive statistics and multivariable logistic regression were used to identify factors associated with TRE adoption, while paired sample t-tests evaluated changes in outcomes among those who received TRE advice. Results: Among the 271 patients, 76% were female, 90% Caucasian, and 94% overweight/obese. Of all patients, 47.2% received TRE advice, mainly using the 16:8 method, alongside additional dietary guidance for 60% of patients. Working status and baseline metabolic profiles were the only factors significantly associated with TRE adoption. Among those who followed TRE, 81% experienced modest but significant reductions in weight (−1.2 kg, p < 0.01), BMI (−0.4 kg/m2, p < 0.01), and waist circumference (−3.7 cm, p < 0.01). Conclusions: This study highlights TRE as a feasible and practical dietary strategy for improving metabolic health in healthcare settings. However, further research and improved data capture are needed to explore long-term adherence, potential adverse effects, and the effectiveness of TRE across diverse patient populations.

Nutrients doi: 10.3390/nu16244427

Authors: Lily Bellehumeur-Béchamp Maxime Legendre Catherine Bégin

Background/Objectives: Binge eating (BE) is associated with physical and psychological consequences, such as obesity and reduced quality of life. The relationship between binge eating and childhood experiences of interpersonal trauma has been explored, yet few studies focus on the processes that may explain this association. In this regard, some personality traits and maladaptive cognitive-emotional regulation may help explain this relationship, as they have been associated, respectively, with BE and childhood interpersonal trauma. The purpose of this study is to explore the complex processes that link childhood interpersonal trauma and BE in a French-Canadian clinical adult population with obesity (BMI ≥ 30 kg/m2). Methods: This cross-sectional study included 148 participants aged 21 to 72, predominantly women of White ethnic background with a university degree, who were seeking treatment for eating or weight-related issues. They completed self-report questionnaires assessing maltreatment and bullying, BE, maladaptive cognitive-emotional regulation, and personality. Two multiple mediation models were tested to examine the indirect effects of personality and maladaptive regulation in the relationship between bullying and BE, as well as between maltreatment and BE. Results: The results revealed a significant indirect relationship between maltreatment and binge eating (BE), with personality traits and maladaptive cognitive-emotional regulation partially explaining this association. No direct effect was found for bullying, but a significant total indirect effect indicated that personality traits and maladaptive cognitive-emotional regulation play a mediating role in the relationship between bullying and BE. Finaly, self-directedness was found as a unique and significant contributor in both mediation models. Conclusions: This study draws attention to the multiple contributing factors in the relationship between interpersonal trauma and BE in adults with obesity. Further research is needed to gain a deeper understanding of the role of personality and maladaptive cognitive-emotional regulation in this relationship by focusing on individuals’ experiences.

Nutrients doi: 10.3390/nu16244425

Authors: Sarai Vásquez-Reyes Miranda Bernal-Gámez Jorge Domínguez-Chávez Karina Mondragón-Vásquez Mónica Sánchez-Tapia Guillermo Ordaz Omar Granados-Portillo Diana Coutiño-Hernández Paulina Barrera-Gómez Nimbe Torres Armando R. Tovar

Background/Objective: In recent studies, it has been shown that dietary bioactive compounds can produce health benefits; however, it is not known whether an improvement in solubility can enhance their biological effects. Thus, the aim of this work was to study whether co-amorphous (CoA) naringenin or fisetin with enhanced solubility modify glucose and lipid metabolism, thermogenic capacity and gut microbiota in mice fed a high-fat, high-sucrose (HFSD) diet. Methods: Mice were fed with an HFSD with or without CoA-naringenin or CoA-fisetin for 3 months. Body weight, food intake, body composition, glucose tolerance, hepatic lipid composition and gut microbiota were assessed. Results: CoA-naringenin demonstrated significant reductions in fat-mass gain, improved cholesterol metabolism, and enhanced glucose tolerance. Mice treated with CoA-naringenin gained 45% less fat mass and exhibited improved hepatic lipid profiles, with significant reductions seen in liver triglycerides and cholesterol. Additionally, both CoA-flavonoids increased oxygen consumption (VO2), contributing to enhanced energy expenditure and improved metabolic flexibility. Thermogenic activation, indicated by increased UCP1 and PGC-1α levels, was observed with CoA-fisetin, supporting its role in fat oxidation and adipocyte size reduction. Further, both CoA-flavonoids modulated gut microbiota, restoring diversity and promoting beneficial bacteria, such as Akkermansia muciniphila, which has been linked to improved metabolic health. Conclusions: These findings suggest that co-amorphous naringenin or fisetin offers promising applications in improving solubility, metabolic health, and thermogenesis, highlighting the potential of both as therapeutic agents against obesity and related disorders.

Nutrients doi: 10.3390/nu16244424

Authors: Hye-Yeon Lee Kyung-Jin Min

Dietary restriction (DR) has been reported to be a significant intervention that influences lipid metabolism and potentially modulates the aging process in a wide range of organisms. Lipid metabolism plays a pivotal role in the regulation of aging and longevity. In this review, we summarize studies on the significant role of lipid metabolism in aging in relation to DR. As a potent intervention to slow down aging, DR has demonstrated promising effects on lipid metabolism, influencing the aging processes across various species. The current review focuses on the relationships among DR-related molecular signaling proteins such as the sirtuins, signaling pathways such as the _target of rapamycin and the insulin/insulin-like growth factor (IGF)-1, lipid metabolism, and aging. Furthermore, the review presents research results on diet-associated changes in cell membrane lipids and alterations in lipid metabolism caused by commensal bacteria, highlighting the importance of lipid metabolism in aging. Overall, the review explores the interplay between diet, lipid metabolism, and aging, while presenting untapped areas for further understanding of the aging process.

Nutrients doi: 10.3390/nu16244423

Authors: Jieun Shin Inhae Kang Myoungsook Lee

This study investigated how the DNACJ6 gene variation related to RMR alteration affects risk factors of obese environments in children with obesity aged 8–9. Methods: Over a three-year follow-up period, 63.3% of original students participated. Changes in the variables (anthropometrics, blood biochemistry, and dietary intakes) were analyzed and compared between those without obesity (non-OB) and with obesity (OB) classified at the study endpoint. Result: The average MAF of nine SNPs (D-1 to D-IX) was defined as 18.1%. The OB group showed greater increases in RMR, BMI, WC, and SBP, while the non-OB group had significantly greater increases in HDL and intakes of nutrients (e.g., total calories, vitamins B2, C, folate, A, retinol, iron, and zinc). Increased RMR, BMI, BW, and RMR/BW changes were observed with mutant allele of D-I SNP, which was also associated with a higher prevalence of obesity. Greater increases in animal fat intake, including saturated fatty acids and retinol, were noted in the minor alleles of D-VI, D-VII, D-VIII, and D-IX SNPs compared to those of the major alleles. The odds ratio for BMI risk was significantly higher in the mutant alleles of D-I (rs17127601), D-VII (rs1334880), and D-VIII (rs7354899) compared to the wild type, with increases of 2.59 times (CI; 1.068–6.274), 1.86 times (CI; 1.012–3.422), and 1.85 times (CI; 1.008–3.416), respectively. RMR was a mild risk factor in minors of the D-1, D-VII, and D-VIII; however, a higher RMR/BW ratio significantly correlated with decreased BMI risk, and this effect was found in only the major alleles of D-I, D-VII, and D-VIII SNPs, not in the minor alleles. High retinol intake appeared to reduce obesity risk in the minor alleles of the D-I, D-VII, and D-VIII SNPs, even though intake of animal fats and retinol remained higher among minors over the three years. Conclusions: These findings suggest that the RMR/BW ratio and dietary fat/retinol intake should be considered in DNACJ6-gene-based precision medicine approaches for pediatric obesity prevention, particularly for boys.

Nutrients doi: 10.3390/nu16244422

Authors: Miwa Tatsuta Tsutomu Masaki Shungo Kimura Yudai Sato Akemi Tomida Ichiro Ishikawa Yu Nakamura Kei Takuma Mai Nakahara Kyoko Oura Tomoko Tadokoro Koji Fujita Joji Tani Asahiro Morishita Takashi Himoto Shinjiro Miyazaki Tsuyoshi Maeta Yoshihiro Mori Fumikazu Kohi Hideki Kobara

Background/Objectives: Metabolic dysfunction-associated steatotic liver disease (MASLD) is diagnosed when hepatic steatosis is proven by imaging and one of the five cardiometabolic criteria is present. The relationship between MASLD and body composition components has recently received increased research attention. However, the five cardiometabolic criteria do not include components of body composition. This study aimed to identify significant body composition factors associated with MASLD in patients undergoing health checkups. Methods: This study included a cohort of 6599 examinees who participated in a health check-up conducted between 2022 and 2023, and their data were prospectively analyzed. The inclusion criteria were undergoing abdominal ultrasonography, alcohol consumption <30 g/day for males or <20 g/day for females, and one of the five cardiometabolic criteria. Results: Finally, 3864 examinees were enrolled. In total, 1133 (51.8%) males and 454 (27.1%) females had MASLD. Sarcopenia was present in only 0.62% of males and 0.66% of females with MASLD. The MASLD group had significantly lower skeletal muscle mass/weight (SMM/WT) values than the non-MASLD group. Multivariate analysis revealed that SMM/WT was independently associated with MASLD. Conclusions: SMM/WT was significantly associated with MASLD. Therefore, muscle mass assessment using SMM/WT may be a potential marker for diagnosing MASLD.

Nutrients doi: 10.3390/nu16244421

Authors: Andrija Karačić Jadran Zonjić Ena Stefanov Katja Radolović Antonio Starčević Ira Renko Željko Krznarić Matija Ivančić Zvonimir Šatalić Ana-Marija Liberati Pršo

Background: Since the gut microbiota is important for athlete health and performance, its optimization is increasingly gaining attention in sports nutrition, for example, with whole fermented foods. Sauerkraut is a traditional fermented food rich in pro-, pre-, and postbiotics, which has not yet been investigated in the field of sports nutrition. Methods: To determine whether sauerkraut could be used for gut microbiota optimization in sports nutrition, a proof-of-concept study was conducted. The microbiota composition of organic pasteurized sauerkraut was analyzed, and then healthy active athletes were provided with the same sauerkraut for 10 days as an intervention. The effects of sauerkraut on the athlete’s gut microbiota, laboratory parameters, and bowel function were assessed. Results: Significant changes in the gut microbiota composition were seen on taxonomic and functional levels, independent of baseline microbiota composition, even after short-term supplementation. Most notably, there was an increase in several health-promoting genera of the family Lachnospiraceae, as well as significant alterations in metabolic pathways regarding cell wall synthesis and the metabolism of nucleotide bases. An increase in the proportion of lymphocytes and a decrease in B12 vitamin levels was observed, as well as a risk of indigestion in certain athletes, which significantly resolved after seven days of supplementation in all athletes. It is unclear whether the observed effects are attributable to the sauerkraut’s own microbiome or its pre- and postbiotics since it is a whole food. Conclusions: Our study has demonstrated that the concept of whole fermented foods, such as sauerkraut, could potentially be feasible and effective in sports nutrition for gut microbiota optimization.

Nutrients doi: 10.3390/nu16244420

Authors: Edda Cava Mauro Lombardo

Malnutrition, a significant risk factor for mortality and morbidity in the elderly, poses a huge threat in the geriatric population, showing a high prevalence, especially in people affected by chronic non-communicable age-related diseases [...]

Nutrients doi: 10.3390/nu16244419

Authors: Serena Rustichelli Cristina Lanni Marta Zarà Gianni Francesco Guidetti Mauro Torti Ilaria Canobbio

Background/Objectives: Amyloid peptides, whose accumulation in the brain as senile plaques is associated with the onset of Alzheimer’s disease, are also found in cerebral vessels and in circulation. In the bloodstream, amyloid peptides promote platelet adhesion, activation, oxidative stress, and thrombosis, contributing to the cardiovascular complications observed in Alzheimer’s disease patients. Natural compounds, such as curcumin, are known to modulate platelet activation induced by the hemostatic stimuli thrombin and convulxin. In this study, we investigated the ability of curcumin to modulate platelet activation triggered by amyloid peptides, and we compared its effects with those displayed on platelet activation induced by physiological agonists. Methods: Commercial ultrapure curcumin was used, and platelet aggregation, granule secretion, phosphorylation of selected signaling proteins, and reactive oxygen species production were analyzed on isolated human platelets. Results: Our results demonstrate that curcumin effectively suppressed platelet aggregation induced by fibrillar amyloid peptides. This effect was associated with the reduction in intracellular signaling pathways involving PKC, PI3K, and MAPK. By contrast, platelet aggregation and activation induced by thrombin and convulxin were only partially reduced by preincubation with curcumin. Moreover, curcumin completely suppressed granule secretion only when platelets were stimulated with hemostatic agonists, but it had no effects upon stimulation with amyloid peptides. Additionally, curcumin reduced the production of reactive oxygen species induced by amyloid peptides with a stronger efficiency compared to platelets stimulated with thrombin. Conclusions: These results indicate that curcumin displays selective and potent inhibitory activity on platelet responses to pathological stimuli, such as fibrillar amyloid peptides.

Nutrients doi: 10.3390/nu16244418

Authors: Isabel Krug An Binh Dang Evonne Lu Wenn Lynn Ooi Jade Portingale Stephanie Miles

Background/Objectives: Recent research has increasingly explored the cognitive processes underlying eating disorders (EDs), including anorexia nervosa (AN), bulimia nervosa (BN), binge eating disorder (BED), other specified feeding or eating disorders (OSFEDs), and individuals with higher weight (HW). This critical narrative review focuses on neurocognitive findings derived from mainly experimental tasks to provide a detailed understanding of cognitive functioning across these groups. Where experimental data are lacking, we draw on self-report measures and neuroimaging findings to offer supplementary insights. Method: A search of major databases that prioritized meta-analyses and recent publications (last 10 years) was conducted. Using comprehensive search terms related to EDs, HW, and neurocognition, eligible studies focused on human neurocognitive outcomes (e.g., cognitive flexibility, attentional bias, etc.) published in English were selected. Results: We found that some neurocognitive characteristics, such as cognitive rigidity, impulsivity, emotion processing difficulties, and dysregulated reward processing, appear transdiagnostic, spanning multiple ED subtypes and HW populations. We also revealed neurocognitive features specific to ED subtypes and HW. For instance, individuals with AN demonstrate an enhanced focus on detail, and BN and BED are characterized by a pronounced attentional bias toward food-related stimuli. In individuals with HW, cognitive processes underpin behaviours associated with overeating and weight gain. Conclusions: These findings highlight the critical importance of understanding both the unique and shared neurocognitive patterns across ED subtypes and HW populations. By identifying transdiagnostic factors, such as cognitive rigidity and reward processing, alongside ED subtype/HW-specific vulnerabilities, researchers and clinicians can develop more nuanced, evidence-based interventions that address the core mechanisms driving disordered eating behaviours.

Nutrients doi: 10.3390/nu16244417

Authors: Yu-Chen Hu Tzu-Ching Huang Bau-Shan Hsieh Li-Wen Huang Jin-Seng Lin Han-Yin Hsu Chia-Chia Lee Kee-Lung Chang

Background: Osteoarthritis (OA) is a chronic condition characterized by joint pain and disability, driven by excessive oxidative stress and inflammatory cytokine production in chondrocytes, resulting in cell death and cartilage matrix breakdown. Our previous study showed that in monosodium iodoacetate (MIA)-induced OA rats, oral administration of heat-killed Lactobacillus delbrueckii subsp. lactis 557 (LDL557) could significantly decrease OA progression. Methods: Accordingly, we designed an in vitro cell culture study aimed at investigating the effects of heat-killed LDL557 extracts on chondrocytes using SW1353 cells (a human chondrosarcoma cell line) challenged with 5 μM MIA to mimic OA conditions. Results: The results showed that the 10 μg/mL LDL557 extracts protected SW1353 cells from MIA-induced death and reduced extracellular matrix (ECM) loss, as evaluated by toluidine blue O staining and extracellular matrix component synthesis with RT-qPCR measurement. This was achieved by decreasing the expression of MIA-induced pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α, while slightly increasing the MIA-suppressed expression of the anti-inflammatory cytokine IL-10, which were evidenced by RT-qPCR analysis. Moreover, the RT-qPCR evaluation also indicated that the LDL557 extracts slightly reduced the expression of COX-2 compared with the control, while it did not reduce the MIA-increased expression of microsomal prostaglandin E synthase-1 (mPGES-1). In addition, the LDL557 extracts influenced neither the matrix-degrading protease expressions measured via RT-qPCR nor the oxidative stress measured via fluorescence flow cytometry in the cells with or without the MIA challenge. Conclusions: This study demonstrates that LDL557 extracts may protect chondrocytes from OA damage by reducing inflammation-related factors and thus mitigating cartilage matrix loss, suggesting LDL557 extracts are attractive alternatives for OA applications.

Nutrients doi: 10.3390/nu16244415

Authors: Aeyung Kim Eun-Ji Lee Jung Ho Han Hwan-Suck Chung

Background/Objectives: Immune checkpoints are essential for regulating excessive autoimmune responses and maintaining immune homeostasis. However, in the tumor microenvironment, these checkpoints can lead to cytotoxic T cell exhaustion, allowing cancer cells to evade immune surveillance and promote tumor progression. The expression of programmed death-ligand 1 (PD-L1) in cancer cells is associated with poor prognoses, reduced survival rates, and lower responses to therapies. Consequently, downregulating PD-L1 expression has become a key strategy in developing immune checkpoint inhibitors (ICIs). Caryophylli cortex (CC), derived from the bark of the clove tree Syzygium aromaticum, possesses antioxidant and cytotoxic properties against cancer cells, yet its potential as an ICI remains unclear. Methods: In this study, we aimed to investigate whether CC extract modulates PD-L1 expression in cancer cells and activates T cell immunity through a co-culture system of cancer cells and T cells, as well as in hPD-L1/MC-38 tumor-bearing animal models. Results: Our findings indicate that CC extract significantly downregulated both constitutive and inducible PD-L1 expression at non-toxic concentrations for cancer cells while simultaneously enhancing cancer cell mortality and T cell activity in the co-culture system. Furthermore, the administration of CC extract to hPD-L1/MC-38 tumor-bearing mice resulted in a greater than 70% reduction in tumor growth and increased infiltration of CD8+ T cells within the tumor microenvironment. Principal component analysis identified bergenin, chlorogenic acid, and ellagic acid as active ICIs. Conclusions: These findings suggest that CC extract exerts a potent antitumor effect as an immune checkpoint blocker by inhibiting PD-L1 expression in cancer cells and disrupting the PD-1/PD-L1 interaction.

Nutrients doi: 10.3390/nu16244416

Authors: Andrew J. Sinclair

The brain is a lipid-rich organ, mainly due to the very high lipid content of myelin, but in addition to this, all the neuronal cell membranes, of which there are over 80 billion in the human brain [...]

Nutrients doi: 10.3390/nu16244414

Authors: Yucheng Zhu Fangyan Wan Jie Liu Zhihao Jia Tongxing Song

Lipids make up 10–20% of the human body and serve as crucial energy storage substances as well as essential components of cell membranes [...]