Extended Data Figure 4: Mutant alleles for the homologues of all five subunits of Paf1C enable siRNAs to induce gene silencing in trans.
From: The Paf1 complex represses small-RNA-mediated epigenetic gene silencing
a, ade6+ siRNAs reduce ade6+ mRNA levels in all Paf1C mutant strains identified in this study. Whole-genome tiling arrays were used to assess gene expression in the mutant cells indicated. y axis is in linear scale. b, C-terminally tagged Tpr1 and Cdc73 are hypomorphic. Full deletions of the tpr1+ and cdc73+ genes cause retarded growth phenotypes (Extended Data Fig. 8c). By contrast, tpr1-3xFLAG and cdc73-3xFLAG grow normally, and display ade6-hp siRNA-mediated repression of the ade6+ gene.
