Pavel Vladimirovich Avdonin

Russian Academy of Sciences | RAS · Koltzov Institute of Developmental Biology

One of the risk factors for infection caused by SARS-CoV-2 is hyperactivation of the complement system, which can lead to activation and damage to the endothelium, thrombosis and, in some severe cases, the development of multiple organ failure. Using quantitative immunohistochemistry, we studied the opsonization of human umbilical vein endothelial...

Damage of the endothelium as a result of activation of the complement system is one of the causes of thrombotic complications in COVID-19. Factor C3 plays a key role in this process. The attachment of its proteolytic product C3b to the cells initiates the formation of the membrane attack complex C5b-9, which forms a pore in the plasma membrane and...

Atypical hemolytic-uremic syndrome (aHUS) is an ultra-rare life-threatening disease from the group of thrombotic microangiopathies (TMA) with genetic predisposition factors associated with uncontrolled activation of the alternative complement pathway. In 10% of cases aHUS is acquired and associated with antibodies to factor H (CFH-Ab-aHUS), among w...

The process of aging is accompanied by a dynamic restructuring of the immune response, a phenomenon known as immunosenescence. Further, damage to the endothelium can be both a cause and a consequence of many diseases, especially in elderly people. The purpose of this study was to carry out immunological and biochemical profiling of elderly people w...

Atypical hemolytic-uremic syndrome (aHUS) is an ultra-rare disease based on the development of complement-mediated thrombotic microangiopathy, which has an unfavorable prognosis in its natural course. The purpose — to determine predictors of unfavorable prognosis of aHUS in children. Material and methods. 70 patients (aged 2.5 months — 16 years) we...

Hemolytic uremic syndrome (HUS) is an acute disease and the most common cause of childhood acute renal failure. HUS is characterized by a triad of symptoms: microangiopathic hemolytic anemia, thrombocytopenia, and acute kidney injury. In most of the cases, HUS occurs as a result of infection caused by Shiga toxin-producing microbes: hemorrhagic Esc...

During the initial diagnosis of urgent medical conditions, which include acute infectious diseases, it is important to assess the severity of the patient’s clinical state as quickly as possible. Unlike individual biochemical or physiological indicators, derived indices make it possible to better characterize a complex syndrome as a set of symptoms,...

Atypical hemolytic uremic syndrome (HUS) develops as a result of damage to the endothelium of microvasculature vessels by Shiga toxin produced by enterohemorrhagic Escherichia coli (STEC-HUS). STEC-HUS remains the leading cause of acute kidney injury (AKI) in children aged 6 months to 5 years. The pathomorphological essence of the disease is the de...

Organophosphates (OP) are one of the most common neurotoxic xenobiotics. In acute OP poisoning, as a result of suppression of synaptic acetylcholinesterase (AChE) activity, a cholinergic syndrome develops, which can transform into status epilepticus. Within a few days after acute poisoning, the so-called an intermediate syndrome can develop, which...

The aim of this work was to explore the involvement of 5-HT1B and 5-HT2B receptors (5-HT1BR and 5-HT2BR) in the regulation of free cytoplasmic calcium concentration ([Ca2+]i) in human umbilical vein endothelial cells (HUVEC). We have shown by quantitative PCR analysis, that 5-HT1BR and 5-HT2BR mRNAs levels are almost equal in HUVEC. Immunofluoresce...

The esterase status of blood plasma can claim to be one of the universal markers of various diseases; therefore, it deserves attention when searching for markers of the severity of COVID-19 and other infectious and non-infectious pathologies. When analyzing the esterase status of blood plasma, the esterase activity of serum albumin, which is the ma...

The new coronavirus infection (COVID-19) pandemic caused by SARS-CoV-2 has many times surpassed the epidemics caused by SARS-CoV and MERS-CoV. The reason for this was the presence of sites in the protein sequence of SARS-CoV-2 that provide interaction with a broader range of receptor proteins on the host cell surface. In this review, we consider bo...

The atypical hemolytic uremic syndrome (aHUS) is an ultra-rare disease with a genetically determined dysregulation of the alternative complement pathway, which leads to the development of thrombotic microangiopathy. The application of the _targeted therapy in Russia has changed the natural course of the disease since 2012: from death of a patient to...

The development of drugs for the therapy of COVID-19 is one of the main problems of modern physiology, biochemistry and pharmacology. Taking into account the available information on the participation of mast cells and the role of histamine in the pathogenesis of COVID-19, as well as information on the positive role of famotidine in the prevention...

[This corrects the article DOI: 10.1134/S0022093022010203.].

Atypical hemolytic uremic syndrome (aHUS) is an orphan disease caused by hyperactivation of the alternative complement pathway. Objective of the study: to assess the state of the «ADAMTS13 – von Willebrand factor (vWF) – platelets» system in children with aHUS. Materials and methods of research: [by the FRET method (fluorescence resonance energy tr...

— Von Willebrand factor (vWF), the key component of hemostasis, is synthesized in endothelial cells and megakaryocytes and released into the blood as high molecular weight multimeric glycoproteins weighing up to 20 million Daltons. Blood plasma metalloprotease ADAMTS13 cleaves ultra-large vWF multimers to smaller multimeric and oligomeric molecules...

Fatigue during exercise is defined as the inability to maintain the required level of strength and performance. Doping agents and manipulation of any kind are undesirable companions of professional and amateur sports. Nutraceuticals have advantages over medicines because they avoid side effects and naturally supplement the diet. In this chapter, ex...

Toxic and mechanistic properties of nutraceuticals are not as extensively studied as those of pharmaceuticals. Flow cytometry is among the most popular techniques to investigate the modes and mechanisms of cytotoxic action of chemical compounds, though flow cytometry methods are not suitable for online registration of rapid changes in cell volumes....

Organosulfur compounds (OSCs) are widely present in our bodies and the natural environment. There are two principal groups of vegetables that contain OSC with special properties. Garlic, onion, shallot, leek, and chives are well-known representatives of the Allium genus (family Amaryllidaceae), that contain S-alk(en)yl-l-cysteine sulfoxides. Cabbag...

Here, we propose a new approach for quantitative estimation of von Willebrand factor (vWF) exposed on the surface of endothelial cells (ECs) using the ARC1779 aptamer that interacts with the vWF A1 domain. To visualize complex formation between vWF and the aptamer, the latter was conjugated with the Cy5 fluorescent label. Cultured human umbilical v...

BACKGROUND: It is now known regulatory effect of gaseous mediators in many bodily functions. These mediators include nitric oxide (NO), carbon monoxide (CO) and hydrogen sulfide (H2S). However, detailed data on the regulatory role of each of these gasotransmitters (GTs) are still not sufficiently studied. OBJECTIVE: The aim of this study was to inv...

Endothelial cells (ECs) line the blood vessels and lymphatic vessels, as well as heart chambers, forming the border between the tissues, on the one hand, and blood or lymph, on the other. Such a strategic position of the endothelium determines its most important functional role in the regulation of vascular tone, hemostasis, and inflammatory proces...

Second messenger nicotinic acid adenine dinucleotide phosphate (NAADP) triggers Ca2+ release via two-pore channels (TPCs) localized in endolysosomal vesicles. The aim of the present work is to evaluate the role of TPCs in the action of norepinephrine (NE), angiotensin II (AngII), vasopressin (AVP), and 5-hydroxytriptamine (5-HT) on free cytoplasmic...

Erythrocytes change their micromechanical properties associated with membrane viscoelasticity when they perform the transport function. There is some evidence that this is due to the influence of signaling molecules, including such gasotransmitters as nitrogen oxide (NO) and hydrogen sulfide (H2S). The aim of this study was to investigate the chang...

Poisoning by organophosphates (OPs) takes one of the leading places in the total number of exotoxicoses. Detoxication of OPs at the first stage of the poison entering the body could be achieved with the help of DNA- or RNA-aptamers, which are able to bind poisons in the bloodstream. The aim of the research was to develop an approach to rational in...

Studying the drugs of natural origin that promote recovery of an organism after acute poisoning with household and industrial toxicants, including organophosphates (OPs), is a challenge not only to toxicology but also informatics, which is a major tool for developing the methodology of system analysis. We report here the data on the effect of decaf...

The long-term effects of acute organophosphate (OP) poisoning remain poorly studied, while experimental models usually disregard species-related specificity of rodents as model objects. Here we present two toxicological models and a comparative analysis of a wide range of biochemical blood indices in their dynamics over 3 months after acute rat poi...

In this study, we investigated the effects of NAD(P)H oxidase (NOX) inhibitor VAS2870 (3-benzyl-7-(2-benzoxazolyl)thio-1,2,3-triazolo[4,5-d]pyrimidine) on the histamine-induced elevation of free cytoplasmic calcium concentration ([Ca2+]i) and the secretion of von Willebrand factor (vWF) in human umbilical vein endothelial cells (HUVECs) and on rela...

Hydrogen peroxide, formed in the endothelium, acts as a factor contributing to the relaxation of blood vessels. The reason for this vasodilatory effect could be modulation by H 2 O 2 of calcium metabolism, since mobilization of calcium ions in endothelial cells is a trigger of endothelium-dependent relaxation. The aim of this work was to investigat...

The currently existing terms “apoptotic index” and “apoptotic potential” are insufficient for the adequate qualitative evaluation and analysis of the proapoptotic activity of chemical agents within the wide range of therapeutic and toxic doses. Herein we introduce a new concept of cytotoxic power as an alternative to these terms related to apoptosi...

Currently existing terms "apoptotic index" and "apoptotic potential" are insufficient for adequate qualitative evaluation and analysis of proapoptotic activity of chemical agents within the wide range of therapeutic and toxic doses. Herein we introduce a new concept of cytotoxic power as an alternative to these terms related to apoptosis and other...

We present a ten-year follow-up of a patient with severe congenital thrombotic thrombocytopenic purpura (TTP), who received permanent replacement therapy with freshly frozen plasma (FFP) transfusions. At presentation, the patient had no activity of plasma ADAMTS-13 and an increased concentration of von Willebrand factor multimers. During treatment,...

Endothelium is a community of endothelial cells (ECs), which line the blood and lymphatic vessels, thus forming an interface between the tissues and the blood or lympha. This strategic position of endothelium infers its indispensable functional role in controlling vasoregulation, haemostasis, and inflammation. The state of endothelium is simultaneo...

The aim of our research was to study the influence of hydrogen peroxide on the exocytosis of von Willebrand factor (vWF) in human umbilical vein endothelial cells (HUVEC). We have found that H2O2 at a non-toxic concentration (100 μM) increases the amount of vWF secreted by HUVEC by 43 ± 14% over control (p < 0.03) and elevates total exposition of v...

Albumin is known as being able to cleave ester bonds in organophosphates (OP) and serve as a major means of OP detoxification due to its large amount in the blood serum. Most of in vivo toxicological studies are conducted on rodents, mice and rats. To adequately extrapolate results of ligand/albumin interaction studies to a human organism, it is ne...

The albumin molecule, in contrast to many other plasma proteins, is not covered with a carbohydrate moiety and can bind and transport various molecules of endogenous and exogenous origin. The enzymatic activity of albumin, the existence of which many scientists perceive skeptically, is much less studied. In toxicology, understanding the mechanistic...

It is known that albumin can break the ester bonds in organophosphorus compounds (OPs). Amino acids responsible for esterase and pseudoesterase activity of albumin towards OPs are still not determined. It is assumed that Sudlow’s site I with residue Tyr150 exhibits the “true” esterase activity; and Sudlow’s site II containing residue Tyr411, a pseu...

Enzymatic activities of three types of serum albumin—rat, bovine and human—were analyzed comparatively using a mathematical model. Kinetic and equilibrium constants of carboxylesterase and paraoxonase activities of albumin in Sudlow’s sites I and II were determined. The effects of specific ligands, ibuprofen and warfarin, on enzyme kinetics in thes...

Hydrogen peroxide at concentrations below cytotoxic ones causes an increase in the cytoplasmic calcium concentration in human umbilical vein endothelial cells as a result of calcium release from intracellular stores. Two-pore calcium channel blocker trans-NED19 partially suppresses the increase in the level of calcium ions in the cells in response...

Albumin is known to be able to cleave ether bonds in organophosphates (OPs). Amino acids responsible for esterase and pseudo-esterase albumin activity towards OPs are not yet finally identified. Presumably, Sudlow’s site I with the Tyr150 residue shows a “true” esterase activity, while Sudlow’s II site with the Tyr411 residue—a pseudo-esterase one....

It has been shown that the Epac1 and Epac2 protein inhibitor ESI-09 has no effect on the amplitude of contraction of aortic rings caused by the influence of serotonin, noradrenaline, or KCl depolarizing solution, but changes the kinetics of the contractile response. It was noted that in the presence of ESI-09 the curve of the relaxation phase in in...

Previously we have shown that at traumatic shock in rats the force of contraction of isolated aorta in response to angiotensin II, vasopressin, endothelin 1, or norepinephrine is decreased. On the contrary, vasoconstriction caused by serotonin is increased. A possible reason of the alterations of neuroendocrine regulation of vascular tone in shock...

Organosulfur compounds (OSC) are widely present in our bodies and the natural environment. There are two principal groups of vegetables that contain OSC with special properties. Garlic, onion, shallot, leek, and chives are well-known representatives of the Allium genus (family Amaryllidaceae) that contain S-alk(en)yl-l-cysteine sulfoxides. Cabbage,...

Fatigue during exercise is defined as the inability to maintain the required level of strength and performance. Doping agents and manipulation of any kind are undesirable companions of professional and amateur sports. Nutraceuticals have an advantage over medicines because they lack side effects and naturally supplement the diet. In this chapter, e...

Toxic and mechanistic properties of nutraceuticals are not as extensively studied as those of pharmaceuticals. Flow cytometry is among the most popular techniques to investigate the modes and mechanisms of cytotoxic action of chemical compounds, although flow cytometry methods are not suitable for online registration of fast changes of cell volumes...

In blood vessels, serotonin 5-HT2B receptors mainly mediate relaxation, although their activation by the selective agonist BW723C86 is known to exert contraction of aorta in deoxycorticosterone acetate (DOCA)-salt and N(omega)-nitro-L-arginine (L-NAME) hypertensive rats [Russel et al., 2002; Banes et al., 2003] and in mice with type 2 diabetes [Nel...

The blocker of two-pore channels trans-NED19 and hydrogen peroxide were found to inhibit histamine-induced relaxation of rat aorta. The degree of inhibition depended on histamine concentration. The relaxation in response to 1 µM histamine of rat aorta preconstricted with 30 mM KCl, serotonin, or endothelin-1, was completely abolished by 30 µM trans...

Human umbilical vein endothelial cells were exposed in culture to hydrogen peroxide (H2O2), keeping them close to physiological conditions (high cell density, high serum content, H2O2 concentration not over 500 µM). Cell viability was assessed by flow cytometry using simultaneous staining with the fluorescent dye PO-PRO-1 to detect early apoptotic...

Action of fluoroacetate (FA) becomes apparent after a latent period, even after exposure to lethal doses. The best-known representative of FA is its sodium salt. There are also series of fluorinated compounds whose metabolism is connected with the formation of FA. To reveal intoxication, adequate analytical procedures, as well as unequivocal bioche...

The ratio of early apoptosis and late apoptosis (necrosis) in the cultured human umbilical vein endothelial cells was estimated after exposure to hydrogen peroxide (H2O2) in vitro trying to keep them close to the physiological conditions (high cell density, high serum content, H2O2 concentration not over 500 μM). Cell viability was assessed using f...

The volume of publications on the role of reactive oxygen species (ROS) in biological processes has been increasing exponentially over the last decades. ROS in large amounts clearly have detrimental effects on cell physiology, whereas low concentrations of ROS are permanently produced in cells and play a role as signaling molecules. An imbalance in...

We describe an infant who developed juvenile myelomonocytic leukemia (JMML) at the age of 6 months. Myeloproliferation was effectively controlled by low-dose cytosine arabinoside and 13-cis retinoic acid therapy. Two years after therapy for JMML was stopped, at the age of 5 years, the patient developed autoimmune thrombotic thrombocytopenic purpura...

Serotonin in cardiovascular system plays an important role in blood coagulation, allergy, and inflammation, as well as in blood vessel tone regulation. In this review, the mechanisms of serotonin effects upon the cells of blood vessels are considered and the list of main agonists and antagonists is presented. The signaling pathways activated by ser...

Arachidonic acid causes an increase in free cytoplasmic calcium concentration ([Ca2+]i) in differentiated skeletal multinucleated myotubes C2C12 and does not induce calcium response in C2C12 myoblasts. The same reaction of myotubes to arachidonic acid is observed in Ca2+-free medium. This indicates that arachidonic acid induces release of calcium i...

Comparative study of the effect of calmodulin inhibitors (trifluoperazine, W-12, and W-13) and the TRPVI channel blocker (capsazepine) on receptor-dependent calcium exchange in smooth muscle cells of the rat aorta and on the contractility of the isolated aorta was conducted. It was determined that trifluoperazine almost completely removes an increa...

In this chapter, we review and highlight recent developments regarding the role of store-operated channels (SOC) and stretch-activated channels (SAC) in skeletal muscle, in the context of Duchenne Muscular Dystrophy (DMD). There is a general consensus that an about twofold increase in Ca2+ influx occurring through SOCs and/or SACs in dystrophic mus...

Comparative study of the effect of calmodulin inhibitors trifluoperazine, W-12, and W-13 and the TRPV1 channel blocker capsazepine on receptor-dependent calcium metabolism in smooth muscle cells of the rat aorta and on the contraction of the isolated rat aorta was performed. Trifluoperazine almost completely abolishes an increase in free cytoplasmi...

Possible involvement ofcalmodulin in adrenergic and serotoninergic regulation of vascular contractility has been studied. Calmodulin inhibitors trifluoperazine and W-13 suppress vasoconstriction of the rat aorta in response to norepinephrine, serotonin, and serotonin 5HT1A- and 5HT2A-receptor agonists (8-OH-DPAT and DOI, respectively) and do not af...

The involvement of calmodulin in adrenergic and serotoninergic regulation of vascular contractility has been studied. Calmodulin inhibitors trifluoperazine and W-13 suppress vasoconstriction of the rat aorta in response to norepinephrine, serotonin, and serotonin 5HT1A and 5HT2A receptor agonists (8-OH-DPAT and DOI, respectively) and do not affect...

Modern data concerning expression, localization, biophysical properties, involvement in calcium regulation, and physiological functions of TRP and Orai channels in skeletal muscle cells are analyzed. In skeletal muscles TRPC1/2/3/4/5/6/7, TRPV2/4, TRPM2/7 and Orai1/3 channels are expressed. Activities of TRPC1/3 and TRPV4 facilitate maximal muscle...

Store-operated Ca2+ entry (SOCE) serves to control essential functions throughout the human body and represents a novel and attractive _target for therapeutic intervention. This book provides an extensive overview of the role of SOCE pathways in Molecular Physiology and Cell Biology, as well as their clinical significance. (Patho)physiological princ...

INTRODUCTION AND AIMS: Recent studies have highlighted that podocyte injury and decrease in glomerular podocyte number leads to proteinuria (PU) and glomerulosclerosis. Urinary detection of podocyte injury by measurement of podocyturia and podocyte-specific protein has been used to estimate ongoing glomerular damage. These urinary tests are widely...

It has been shown that the agonist of 5HT1A-receptors 8-OH-DPAT induces contraction of aortic rings in the presence of angiotensin II. This effect is not associated with activation of alpha1-adrenoceptors by 8-OH-DPAT as it is reproduced in the presence of prazosin which completely suppresses the nonspecific vasoconstrictive effect of 8-OH-DPAT via...

The expression levels of inositol-1,4,5-trisphosphate receptors (InsP3R) and cytoplasmic free calcium concentration ([Ca2+]c) changes in response to ATP in cultured skeletal myoblasts C2C12, in myoblasts at different stages of differentiation into myotubes, and in differentiated multinucleated myotubes have been determined. Using reverse transcript...

Agonist 5HT1A serotonin receptors 8-OH-DPAT at 70–80% in rats relax the isolated aorta and mesenteric artery, precollapsed with noradrenaline. An inhibitor of NO-synthase L-NAME two or more times suppresses vasodilatatornyh reaction in response to the effect of 8-OH-DPAT. The addition of 8-OH-DPAT to the aorta in a state of rest or precollapsed wit...

Agonist 5HT1A serotonin receptors 8-OH-DPAT at 70-80% in rats relax the isolated aorta and mesenteric artery, precollapsed with noradrenaline. An inhibitor of NO-synthase L-NAME two or more times suppresses vazodilatatomyh reaction in response to the effect of 8-OH-DPAT. The addition of 8-OH-DPAT to the aorta in a state of rest or precollapsed with...

ISSN 1990-7478, Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology, 2009, Vol. 3, No. 3, p. 299. © Pleiades Publishing, Ltd., 2009.Original Russian Text © P.V. Avdonin, I.B. Sukhanova, K.V. Surkov, O. Petermann, U.T. Rueggc, 2009, published in Biologicheskie Membrany, 2009, Vol. 26, No. 4, p. 305.

The goal of this work was to study possible mechanisms underlying the potentiation of vasopressor response to serotonin observed in traumatic shock. Experiments with isolated aorta and mesenteric artery of the rat showed that vasoconstriction is caused by the activation of 5HT2A receptors. Agonists of 5HT1B, 5HT1D, 5HT2B, and 5HT4 receptors induced...

Expression of channel protein Orai-1 has been demonstrated in cultured skeletal myoblasts and myotubes. In order to evaluate its functional role in Ca2+ transport in these cells we used small interfering RNA (siRNA) _targeted against mRNA coding for Orai-1. Optimal conditions for the effective transfection of myoblasts and myotubes were found. In ex...

It has been shown that. channel protein Orai-1 is expressed in Cultured mice skeletal myoblasts and myotubes C2C12 and mdx edl. In order to determine its functional role in Ca(2+) transport. in these cells we Used short interfering RNA (siRNA) _targeted against. mRNA of Orai-1. Conditions of effective transfection of siRNA into myoblasts and myotubu...

Normally serotonin reduced blood pressure. It was shown that in rats with traumatic shock its hypotensive effect was transformed into hypertensive one. In vitro serotonin exhibited a slight vasoconstrictor effect on isolated rat aorta, while 24 h after injury, the strength of aortic contractions in response to serotonin increased 2.2 times. Desensi...

The mechanisms of cAMP action on the contractility of the isolated heart were studied in the snail Helix pomatia. Serotonin is a powerful activator of heart contractility in this animal. Preincubation of the isolated heart ventricle with the activator of protein kinase A (PKA) Sp-8-bromoadenosine-3′,5′-cyclic monophosphothioate (200 μM) or the acti...

Thrombin acts on mammalian cells through the specific, so-called protease-activated receptors (PARs). The thrombin action is mediated via three out of four known types of these receptors—PAR1,3,4. Mammalian thrombin receptors, apart from performance of other functions, control cardiac and vascular contractility. It is not known whether receptors of...

Membrane receptors are known to be _targets of the majority of modern drugs. However, alterations of these receptors in diseases, causes of these alterations, and their relations with the development of diseases are studied only in few cases. This review considers some mechanisms of regulation of activity and expression of G-protein-coupled receptor...

The fact that the activity of cytosol glucocorticoid receptors decreases in shock have been shown before [Golikov P. P. et al., 2001]. The connection between the development of vascular hyporeactivity to endogenous vasoconstrictors and desensitization of glucocorticoid receptors was studied in this investigation. On Kenton traumatic model in a rat...

Thrombin acts on mammalian cells through specific, the so-called protease-activated receptors (PARs). The thrombin action is mediated via three out of four known types of these receptors PAR(1, 3, 4). Mammalian thrombin receptors, apart from performance of other functions, control cardiac and vascular contractility. It is not known whether receptor...

Without Abstract

Changes in hormonal regulation of the vascular tone in Wistar rats were studied on Cannon model of traumatic shock. The pressor response to angiotensin II decreased by 30-40% 3 h after the incidence of trauma. The reaction to vasopressin remained unchanged. However, phenylephrine in medium and high doses produced a more pronounced pressor response...

RNA interference is a mechanism of posttranslational (at the level of mRNA) gene silencing. Sequence-specific mRNA degradation is realized with the help of small interfering RNAs produced by processing of a precursor using Dicer, an enzyme from the RNAse III family. This mechanism is now widely used in vitro on cultures of mammalian cells in order...

To investigate genetic factors of risk (RF) to develop venous thrombosis and pulmonary artery thromboembolism (PATE) in population of central Russia. We studied polymorphism of the genes of coagulation factor II (G20210A), factor V (G1691A) and methylentetrahydrofolatereductase (MTHFR) with polymerase chain reaction and restriction analysis of DNA...

To specify detectability and clinical presentation of antiphospholipid syndrome (APS) in young and middle aged patients with phlebothromboses (PT). Enzyme immunoassays for lupus anticoagulant, PCR determination of G1691A mutation in the gene of coagulation factor V, mutation G20210A in prothrombin gene, mutation C677T in methylenetetrahydrofolate r...

Thelargest group of cellular receptors is constituted by so-called G protein-coupled receptors (GPCR), which are also known as heptahelical receptors. GPCR are divided into five main families and their functions include not only response to environmental and extracellular factors, but also interaction with extracellular matrix. The latter function...

We have shown that peptide ligands of type 1 and type 4 proteinase-activated receptors (PAR(1) and PAR(4)) stimulate contractile activity of isolated uterus rings of adult mice and do not induce contraction of uterus rings from immature mice. Using reverse transcription and polymerase chain reaction (RT-PCR) the presence of PAR(1) and PAR(4) mRNA w...

Ultradian protein synthesis rhythm was used as a marker of cell cooperation in synchronous dense and non-synchronous sparse hepatocyte cultures. Phenylephrine (2 microM, 2 min), an alpha (1)-adrenoreceptor agonist, which exerts [Ca(2+)](cyt)elevation from intracellular stores, affected protein synthesis rhythm in sparse cultures, i.e. initiated coo...